Preharvest soybean meal supplementation alters tissue-specific protein and amino acid composition in Morchella sextelata: integrated transcriptomic and proteomic analysis
- 1National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences , ,
- 2Amway (China) Botanical Research and Development Center , , ,
Abstract
Preharvest nutrient inputs can modify mushroom composition, but whether graded supplementation differentially affects the protein and amino acid composition of anatomically distinct fruiting-body tissues remains unclear. This study aimed to characterize the compositional and molecular responses of the cap and stipe of Morchella sextelata to additional soybean meal. Protein and 18 hydrolyzed amino acids were quantified following 0–10% supplementation, while the control, 2%, 4%, and 8% treatments were analyzed by transcriptomics and proteomics. Protein content responded nonlinearly and was highest at 8% in both tissues; total and essential amino acid contents were also highest in 8% caps. However, cap protein-normalized essential amino acid proportionality did not improve correspondingly, distinguishing compositional enrichment from amino acid balance. Caps generally exhibited more differentially expressed genes, whereas stipes contained more differentially expressed proteins, with downregulated proteins predominating in the 2 and 8% stipe comparisons. Integrated analyses associated these responses with carbon and amino acid metabolism, translation, protein homeostasis, energy metabolism, and RNA processing. These findings demonstrate tissue- and molecular-layer-dependent responses to preharvest nutrient supplementation. Future studies should determine whether the associated pathways translate into altered enzyme activities, metabolic fluxes, and sensory-relevant metabolites.
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BibTeX
@article{yue2026preharvest,
title = {{Preharvest soybean meal supplementation alters tissue-specific protein and amino acid composition in Morchella sextelata: integrated transcriptomic and proteomic analysis}},
author = {Yihong Yue and Qian Wang and Yuchen Zhang and Tingting Xiao and Haibo Hao and Zongjun Tong and Wei Li and Jingjing Huang and Jinjing Zhang and Hui Chen},
journal = {Frontiers in nutrition},
year = {2026},
month = oct,
doi = {10.3389/fnut.2026.1979592},
url = {https://europepmc.org/article/PMC/PMC13724355},
note = {CC BY},
}
RIS
TY - JOUR
TI - Preharvest soybean meal supplementation alters tissue-specific protein and amino acid composition in Morchella sextelata: integrated transcriptomic and proteomic analysis
AU - Yihong Yue
AU - Qian Wang
AU - Yuchen Zhang
AU - Tingting Xiao
AU - Haibo Hao
AU - Zongjun Tong
AU - Wei Li
AU - Jingjing Huang
AU - Jinjing Zhang
AU - Hui Chen
PY - 2026
DA - 2026/10/19
JO - Frontiers in nutrition
DO - 10.3389/fnut.2026.1979592
UR - https://europepmc.org/article/PMC/PMC13724355
AB - Preharvest nutrient inputs can modify mushroom composition, but whether graded supplementation differentially affects the protein and amino acid composition of anatomically distinct fruiting-body tissues remains unclear. This study aimed to characterize the compositional and molecular responses of the cap and stipe of Morchella sextelata to additional soybean meal. Protein and 18 hydrolyzed amino acids were quantified following 0–10% supplementation, while the control, 2%, 4%, and 8% treatments were analyzed by transcriptomics and proteomics. Protein content responded nonlinearly and was highest at 8% in both tissues; total and essential amino acid contents were also highest in 8% caps. However, cap protein-normalized essential amino acid proportionality did not improve correspondingly, distinguishing compositional enrichment from amino acid balance. Caps generally exhibited more differentially expressed genes, whereas stipes contained more differentially expressed proteins, with downregulated proteins predominating in the 2 and 8% stipe comparisons. Integrated analyses associated these responses with carbon and amino acid metabolism, translation, protein homeostasis, energy metabolism, and RNA processing. These findings demonstrate tissue- and molecular-layer-dependent responses to preharvest nutrient supplementation. Future studies should determine whether the associated pathways translate into altered enzyme activities, metabolic fluxes, and sensory-relevant metabolites.
SN - 2296-861X
ER -