Formulation-dependent colloidal properties of redispersible lycopene nanoparticles: storage stability and enhanced in vitro bioactivity.
- 1Institute of Chemistry, Vietnam Academy of Science and Technology 18 Hoang Quoc Viet, Nghia Do Ward Hanoi 10000 Vietnam [email protected].
- 2Graduate University of Science and Technology, Vietnam Academy of Science and Technology 18 Hoang Quoc Viet, Nghia Do Ward Hanoi 10000 Vietnam.
Abstract
Lycopene is a bioactive carotenoid whose application is limited by poor aqueous dispersibility and susceptibility to degradation. Redispersible lycopene nanoparticles were prepared by solvent displacement-evaporation followed by freeze-drying, and interfacial composition and nominal lycopene fraction were evaluated sequentially. Among the tested interfacial compositions, a 2 : 1 Tween 80/phosphatidylcholine mass ratio produced the smallest particles and lowest dispersity. At this ratio, the formulation containing 12 wt% nominal lycopene was selected for its balance of lycopene content, particle size, and dispersity. The measured lycopene content of the freeze-dried formulation was 11.63 wt%; after aqueous redispersion, the particles exhibited a mean hydrodynamic diameter of 133.25 ± 1.46 nm, a polydispersity index of 0.173 ± 0.016, and a zeta potential of -38.3 ± 1.9 mV. TEM showed predominantly near-spherical particles. FTIR spectra were consistent with physical incorporation in the matrix, while XRD showed marked attenuation of crystalline lycopene reflections. The redispersed formulation maintained its colloidal characteristics for 7 days at 4 °C, and the freeze-dried formulation retained 91.9% of its initial lycopene content after 180 days at -20 °C. At matched lycopene doses, the formulation showed higher apparent DPPH radical-scavenging activity and greater protection against H₂O₂-induced loss of HepG2 cell viability than free lycopene. It also inhibited oleic acid-induced intracellular lipid accumulation by 89.1% at the highest dose versus 68.0% for free lycopene, while cell viability remained above 90%. Overall, sequential evaluation of interfacial composition and nominal lycopene fraction yielded a redispersible freeze-dried formulation combining controlled colloidal properties, storage stability, and improved in vitro functional performance.
Related
- Recent advances in metallic nanoparticles: From characterization to toxicity and bioactivity
- Effect of chitosan and aragonite derived from cuttlebone waste on the bioactivity of bioactive glass nanoparticles (55S5): Physicochemical evaluation and cytotoxic investigations
- Effect of extraction temperature on the fatty acid composition, metabolic profile, and bioactivity-related properties of Lepidium sativum seed oil
- Solubilizer-Assisted Formulation of Porphyrin-Containing Lipid Nanoparticles for Photodynamic Applications
- Hydrogel Formulation Containing PLGA Nanoparticles for Controlled Codelivery of Vitamin D and Chlorhexidine as a Promising Approach for Postdental Implant Applications
BibTeX
@article{oanh2026formulationdependent,
title = {{Formulation-dependent colloidal properties of redispersible lycopene nanoparticles: storage stability and enhanced in vitro bioactivity.}},
author = {Ho Thi Oanh and Hong Tham Nguyen and Nguyen Phu Duc and Nhung Hac Thi and Dinh Dung Luong and Mai Ha Hoang},
journal = {RSC advances},
year = {2026},
month = oct,
doi = {10.1039/d6ra08147a},
url = {https://europepmc.org/article/MED/42898975},
note = {CC BY},
}
RIS
TY - JOUR
TI - Formulation-dependent colloidal properties of redispersible lycopene nanoparticles: storage stability and enhanced in vitro bioactivity.
AU - Ho Thi Oanh
AU - Hong Tham Nguyen
AU - Nguyen Phu Duc
AU - Nhung Hac Thi
AU - Dinh Dung Luong
AU - Mai Ha Hoang
PY - 2026
DA - 2026/10/19
JO - RSC advances
DO - 10.1039/d6ra08147a
UR - https://europepmc.org/article/MED/42898975
AB - Lycopene is a bioactive carotenoid whose application is limited by poor aqueous dispersibility and susceptibility to degradation. Redispersible lycopene nanoparticles were prepared by solvent displacement-evaporation followed by freeze-drying, and interfacial composition and nominal lycopene fraction were evaluated sequentially. Among the tested interfacial compositions, a 2 : 1 Tween 80/phosphatidylcholine mass ratio produced the smallest particles and lowest dispersity. At this ratio, the formulation containing 12 wt% nominal lycopene was selected for its balance of lycopene content, particle size, and dispersity. The measured lycopene content of the freeze-dried formulation was 11.63 wt%; after aqueous redispersion, the particles exhibited a mean hydrodynamic diameter of 133.25 ± 1.46 nm, a polydispersity index of 0.173 ± 0.016, and a zeta potential of -38.3 ± 1.9 mV. TEM showed predominantly near-spherical particles. FTIR spectra were consistent with physical incorporation in the matrix, while XRD showed marked attenuation of crystalline lycopene reflections. The redispersed formulation maintained its colloidal characteristics for 7 days at 4 °C, and the freeze-dried formulation retained 91.9% of its initial lycopene content after 180 days at -20 °C. At matched lycopene doses, the formulation showed higher apparent DPPH radical-scavenging activity and greater protection against H₂O₂-induced loss of HepG2 cell viability than free lycopene. It also inhibited oleic acid-induced intracellular lipid accumulation by 89.1% at the highest dose versus 68.0% for free lycopene, while cell viability remained above 90%. Overall, sequential evaluation of interfacial composition and nominal lycopene fraction yielded a redispersible freeze-dried formulation combining controlled colloidal properties, storage stability, and improved in vitro functional performance.
SN - 2046-2069
ER -