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Peer-reviewedCC BY 4.0Scientific Reports

Defining the levels of the chromatin landscape using the I-CATCH system

  • Bradley M. Downs1
  • Jiumei Hu2
  • Joon Soo Park3
  • Hanran Lei4
  • Tra My Hoang5
  • Thomas R. Pisanic1,6
  • Tza-Huei Wang1,2,4
6 affiliations
  1. 1Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD 21218 USA
  2. 2Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218 USA
  3. 3Department of Chemistry, Konkuk University, Seoul, 05029 South Korea
  4. 4Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218 USA
  5. 5MilliporeSigma, 9820 Darnestown Rd, Rockville, MD 20850 USA
  6. 6Department of Oncology, The Sidney Kimmel Cancer Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA
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Abstract

Alterations of the epigenome, including histone tail post-translational modifications (PTMs), have been shown to regulate the expression of genes important for driving multiple cellular processes including cellular differentiation, aging and tumorigenesis. Although the ability to measure histone tail PTM levels is integral to the understanding of chromatin biology, because of technological limitations, the proportion of nucleosomes that have histone tail PTMs at genomic loci remain undefined. To overcome this limitation, we developed the immuno-profiling of dCas9 targeted chromatin (I-CATCH) system, a technology designed to measure and normalize the levels of nucleosomes and co-occurring histone tail PTMs at targeted loci. Here, we show that the I-CATCH system has high analytical performance for measuring the levels of histone tail PTMs and we report the normalized levels of multiple histone tail PTMs at different genomic loci from both HeLa cells and human plasma. While further studies are required to determine the full utility of the I-CATCH system, we are hopeful that the I-CATCH system will help enable the ability to investigate new important features of chromatin biology.

Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1038/s41598-026-65001-w.

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Cite

BibTeXRIS
BibTeX
@article{downs2026defining,
  title = {{Defining the levels of the chromatin landscape using the I-CATCH system}},
  author = {Bradley M. Downs and Jiumei Hu and Joon Soo Park and Hanran Lei and Tra My Hoang and Thomas R. Pisanic and Tza-Huei Wang},
  journal = {Scientific Reports},
  year = {2026},
  month = aug,
  doi = {10.1038/s41598-026-65001-w},
  url = {https://doi.org/10.1038/s41598-026-65001-w},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Defining the levels of the chromatin landscape using the I-CATCH system
AU  - Bradley M. Downs
AU  - Jiumei Hu
AU  - Joon Soo Park
AU  - Hanran Lei
AU  - Tra My Hoang
AU  - Thomas R. Pisanic
AU  - Tza-Huei Wang
PY  - 2026
DA  - 2026/08/06
JO  - Scientific Reports
DO  - 10.1038/s41598-026-65001-w
UR  - https://doi.org/10.1038/s41598-026-65001-w
AB  - Alterations of the epigenome, including histone tail post-translational modifications (PTMs), have been shown to regulate the expression of genes important for driving multiple cellular processes including cellular differentiation, aging and tumorigenesis. Although the ability to measure histone tail PTM levels is integral to the understanding of chromatin biology, because of technological limitations, the proportion of nucleosomes that have histone tail PTMs at genomic loci remain undefined. To overcome this limitation, we developed the immuno-profiling of dCas9 targeted chromatin (I-CATCH) system, a technology designed to measure and normalize the levels of nucleosomes and co-occurring histone tail PTMs at targeted loci. Here, we show that the I-CATCH system has high analytical performance for measuring the levels of histone tail PTMs and we report the normalized levels of multiple histone tail PTMs at different genomic loci from both HeLa cells and human plasma. While further studies are required to determine the full utility of the I-CATCH system, we are hopeful that the I-CATCH system will help enable the ability to investigate new important features of chromatin biology.  Supplementary Information: The online version contains supplementary material available at https://doi.org/10.1038/s41598-026-65001-w.
SN  - 2045-2322
ER  -