Immunoglobulins and Efgartigimod reduce neuronal activity in primary rat cortical neuron cultures
- 1Department of Neurology, University of Cologne, Faculty of Medicine and University Hospital, Cologne, Germany
- 2Cognitive Neuroscience, Institute of Neuroscience and Medicine (INM-3), Research Centre Juelich, Juelich, Germany
Abstract
Immunoglobulin-based therapies are increasingly used to modulate neuroinflammatory diseases, yet it remains unclear whether they directly affect neuronal function. Here, we provide evidence that primary rat cortical neurons express both the inhibitory Fc gamma receptor IIb (FcγRIIb) and the neonatal Fc receptor (FcRn), as assessed by immunocytochemistry and mRNA analysis. FcγRIIb expression was upregulated under proinflammatory conditions, indicating inflammation-dependent regulation. Using microelectrode array recordings, we observed that immunoglobulin treatment reduced neuronal activity under both physiological and inflammatory conditions. Similarly, Efgartigimod, a high-affinity FcRn blocker, was associated with a sustained reduction in neuronal activity, with the observed effects suggesting a trend toward dose dependence under control conditions. Together, these findings provide proof-of-concept evidence that primary cortical neuronal cultures respond to immunoglobulins and Efgartigimod in vitro and highlight neuronal Fc receptor signaling as a promising avenue for future studies.
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BibTeX
@article{bautista2026immunoglobulins,
title = {{Immunoglobulins and Efgartigimod reduce neuronal activity in primary rat cortical neuron cultures}},
author = {Felix Marti Bautista and Nilufar Nazarzadeh and Niklas Genseke and Elena Groß and Daniel Navin Olschewski and Daniel Navin Olschewski and Gereon Rudolph Fink and Gereon Rudolph Fink and Michael Schroeter and Maria Adele Rueger and Maria Adele Rueger and Sabine Ulrike Vay},
journal = {Frontiers in Cellular Neuroscience},
year = {2026},
month = oct,
doi = {10.3389/fncel.2026.1939772},
url = {https://doi.org/10.3389/fncel.2026.1939772},
note = {CC BY},
}
RIS
TY - JOUR
TI - Immunoglobulins and Efgartigimod reduce neuronal activity in primary rat cortical neuron cultures
AU - Felix Marti Bautista
AU - Nilufar Nazarzadeh
AU - Niklas Genseke
AU - Elena Groß
AU - Daniel Navin Olschewski
AU - Daniel Navin Olschewski
AU - Gereon Rudolph Fink
AU - Gereon Rudolph Fink
AU - Michael Schroeter
AU - Maria Adele Rueger
AU - Maria Adele Rueger
AU - Sabine Ulrike Vay
PY - 2026
DA - 2026/10/28
JO - Frontiers in Cellular Neuroscience
DO - 10.3389/fncel.2026.1939772
UR - https://doi.org/10.3389/fncel.2026.1939772
AB - Immunoglobulin-based therapies are increasingly used to modulate neuroinflammatory diseases, yet it remains unclear whether they directly affect neuronal function. Here, we provide evidence that primary rat cortical neurons express both the inhibitory Fc gamma receptor IIb (FcγRIIb) and the neonatal Fc receptor (FcRn), as assessed by immunocytochemistry and mRNA analysis. FcγRIIb expression was upregulated under proinflammatory conditions, indicating inflammation-dependent regulation. Using microelectrode array recordings, we observed that immunoglobulin treatment reduced neuronal activity under both physiological and inflammatory conditions. Similarly, Efgartigimod, a high-affinity FcRn blocker, was associated with a sustained reduction in neuronal activity, with the observed effects suggesting a trend toward dose dependence under control conditions. Together, these findings provide proof-of-concept evidence that primary cortical neuronal cultures respond to immunoglobulins and Efgartigimod in vitro and highlight neuronal Fc receptor signaling as a promising avenue for future studies.
SN - 1662-5102
ER -