The First Report of a Bang-Sensitive Strain in Drosophila funebris: Molecular-Genetic and Lipidomic Characterization
4 affiliations
- 1Vavilov Institute of General Genetics Russian Academy of Sciences, Gubkin St. 3, 119991 Moscow, Russia
- 2Mental Health Research Center, Clinical Genetics Laboratory, Kashirskoe Sh. 34, 115522 Moscow, Russia
- 3Department of Postgenomics, Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, 119435 Moscow, Russia
- 4National Research Centre “Kurchatov Institute”, 2 Kurchatov Sq., 123182 Moscow, Russia
Abstract
Bang sensitivity (BS) is a well-characterized seizure-and-paralysis phenotype in Drosophila melanogaster, but its genetic basis remains unexamined in the phylogenetically distant sister species Drosophila funebris. Here, we identify a naturally occurring BS strain in D. funebris from a wild population near Lake Valday, characterized by acute mechanical shock-induced seizures and paralysis, and examine its molecular-genetic and lipidomic basis. Comparative transcriptomics identified 241 differentially expressed genes (FDR-adjusted p < 0.05), with “fatty acid metabolic process” as the most enriched GO term. Notably, within this pathway, the peroxisomal acyl-CoA oxidase gene Acox57D-d showed the most significant downregulation (log2FC = −4.21, padj = 4.1 × 10−110), validated by head-specific RT-qPCR. Untargeted lipidomics of BS heads (LC-Q-TOF-MS) revealed 145 altered lipid species, dominated by oxygenated triacylglycerol accumulation, cardiolipin/phosphatidylethanolamine depletion, and a divergent shift—depletion of DG 29:0 and accumulation of DG 47:4, reflecting impaired peroxisomal β-oxidation and potential mitochondrial and Na+/K+-ATPase dysfunction. These findings nominate Acox57D-d as a leading candidate gene associated with the phenotype and molecular signature of the studied BS line and establish this natural BS strain as a potentially useful model for investigating the relationship between peroxisomal lipid metabolism, neuronal excitability, and insect thanatosis.
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BibTeX
@article{kosherova2026first,
title = {{The First Report of a Bang-Sensitive Strain in Drosophila funebris: Molecular-Genetic and Lipidomic Characterization}},
author = {Karina A. Kosherova and Dmitrii A. Abashkin and Natalya B. Zakharzhevskaya and Victoriia D. Kazakova and Elizaveta A. Vorobyeva and Alexander V. Symonenko and Dmitry V. Mukha},
journal = {International journal of molecular sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27198845},
url = {https://europepmc.org/article/PMC/PMC13665471},
note = {CC BY},
}
RIS
TY - JOUR
TI - The First Report of a Bang-Sensitive Strain in Drosophila funebris: Molecular-Genetic and Lipidomic Characterization
AU - Karina A. Kosherova
AU - Dmitrii A. Abashkin
AU - Natalya B. Zakharzhevskaya
AU - Victoriia D. Kazakova
AU - Elizaveta A. Vorobyeva
AU - Alexander V. Symonenko
AU - Dmitry V. Mukha
PY - 2026
DA - 2026/10/01
JO - International journal of molecular sciences
DO - 10.3390/ijms27198845
UR - https://europepmc.org/article/PMC/PMC13665471
AB - Bang sensitivity (BS) is a well-characterized seizure-and-paralysis phenotype in Drosophila melanogaster, but its genetic basis remains unexamined in the phylogenetically distant sister species Drosophila funebris. Here, we identify a naturally occurring BS strain in D. funebris from a wild population near Lake Valday, characterized by acute mechanical shock-induced seizures and paralysis, and examine its molecular-genetic and lipidomic basis. Comparative transcriptomics identified 241 differentially expressed genes (FDR-adjusted p < 0.05), with “fatty acid metabolic process” as the most enriched GO term. Notably, within this pathway, the peroxisomal acyl-CoA oxidase gene Acox57D-d showed the most significant downregulation (log2FC = −4.21, padj = 4.1 × 10−110), validated by head-specific RT-qPCR. Untargeted lipidomics of BS heads (LC-Q-TOF-MS) revealed 145 altered lipid species, dominated by oxygenated triacylglycerol accumulation, cardiolipin/phosphatidylethanolamine depletion, and a divergent shift—depletion of DG 29:0 and accumulation of DG 47:4, reflecting impaired peroxisomal β-oxidation and potential mitochondrial and Na+/K+-ATPase dysfunction. These findings nominate Acox57D-d as a leading candidate gene associated with the phenotype and molecular signature of the studied BS line and establish this natural BS strain as a potentially useful model for investigating the relationship between peroxisomal lipid metabolism, neuronal excitability, and insect thanatosis.
SN - 1422-0067
ER -