In Vitro Modulatory Effect of Stimulant Beverages on the Growth Kinetics of Intestinal Bacteria Associated with Colorectal Cancer.
- 1Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamycka 129, Praha-Suchdol, 165 00 Prague, Czech Republic.
- 2Institute for Obesity Research and School of Medicine and Health Sciences, Tecnologico de Monterrey, Monterrey 64849, Mexico.
Abstract
Colorectal cancer is increasingly linked to diet-induced gut microbiota modulations, whereas the role of oncogenic bacteria is discussed. This study investigated the in vitro growth-modulatory effects of hot-water extracts of six stimulant beverages, namely Aspalathus linearis (rooibos), Coffea arabica, Coffea canephora (coffee), Camellia sinensis var. assamica, C. sinensis var. sinensis (tea) and Ilex paraguariensis (yerba mate) against six colorectal cancer-associated bacteria. Although no minimum inhibitory concentration (MIC) was detected (MIC > 512 µg/mL), growth kinetics analysis revealed modulations in the case of several strains. At the concentration 512 µg/mL, C. sinensis var. assamica and I. paraguariensis modulated the growth (41-56%) and prolonged the lag phase (61-128 min) of Clostridium septicum. The same concentration of both extracts modulated Fusobacterium necrophorum (26-27%), whereas I. paraguariensis extended the lag phase by 72 and 21 min in the cases of Peptostreptococcus anaerobius and Escherichia coli, respectively. Both C. sinensis varieties also reduced the growth of Streptococcus bovis (49-60%), while some treatments stimulated the growth of E. coli. These findings demonstrate in vitro growth modulation of selected colorectal cancer-associated bacteria, especially C. sinensis and I. paraguariensis, and warrant validation using multiple strains, independently replicated assays, chemically characterized extracts, and more physiologically relevant microbial models.
Related
- Portulaca oleracea L.-derived extracellular vesicle-like particles reprogram intestinal ILCs to alleviate inflammatory bowel disease and colitis-associated colorectal cancer
- Replacing animal-sourced with plant-sourced proteins alters gut microbiota and biomarkers associated with colorectal cancer risk in healthy adults: A randomized feeding study
- SLC35D1 Is Associated with Colorectal Cancer Progression, EMT-Related Phenotypes, and Immune Infiltration
- Gut microbiome features associated with PIK3CA-mutated colorectal cancer: A nationwide pan-cancer genomic and microbial profiling study (MONSTAR-SCREEN)
- Postelectroacupuncture Fecal Microbiota Transplantation (post‐EA FMT) Regulates Gastrointestinal Hormones in Functional Dyspepsia Rats Is Associated With Modulation of the Intestinal Microbiota‐Bile Acid Metabolic Pathway
BibTeX
@article{kudera2026vitro,
title = {{In Vitro Modulatory Effect of Stimulant Beverages on the Growth Kinetics of Intestinal Bacteria Associated with Colorectal Cancer.}},
author = {Tomas Kudera and Diana Karina Baigts-Allende and Barbora Fiserova and Ladislav Kokoska},
journal = {Foods (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/foods15193532},
url = {https://europepmc.org/article/MED/42883639},
note = {CC BY},
}
RIS
TY - JOUR
TI - In Vitro Modulatory Effect of Stimulant Beverages on the Growth Kinetics of Intestinal Bacteria Associated with Colorectal Cancer.
AU - Tomas Kudera
AU - Diana Karina Baigts-Allende
AU - Barbora Fiserova
AU - Ladislav Kokoska
PY - 2026
DA - 2026/10/03
JO - Foods (Basel, Switzerland)
DO - 10.3390/foods15193532
UR - https://europepmc.org/article/MED/42883639
AB - Colorectal cancer is increasingly linked to diet-induced gut microbiota modulations, whereas the role of oncogenic bacteria is discussed. This study investigated the in vitro growth-modulatory effects of hot-water extracts of six stimulant beverages, namely Aspalathus linearis (rooibos), Coffea arabica, Coffea canephora (coffee), Camellia sinensis var. assamica, C. sinensis var. sinensis (tea) and Ilex paraguariensis (yerba mate) against six colorectal cancer-associated bacteria. Although no minimum inhibitory concentration (MIC) was detected (MIC > 512 µg/mL), growth kinetics analysis revealed modulations in the case of several strains. At the concentration 512 µg/mL, C. sinensis var. assamica and I. paraguariensis modulated the growth (41-56%) and prolonged the lag phase (61-128 min) of Clostridium septicum. The same concentration of both extracts modulated Fusobacterium necrophorum (26-27%), whereas I. paraguariensis extended the lag phase by 72 and 21 min in the cases of Peptostreptococcus anaerobius and Escherichia coli, respectively. Both C. sinensis varieties also reduced the growth of Streptococcus bovis (49-60%), while some treatments stimulated the growth of E. coli. These findings demonstrate in vitro growth modulation of selected colorectal cancer-associated bacteria, especially C. sinensis and I. paraguariensis, and warrant validation using multiple strains, independently replicated assays, chemically characterized extracts, and more physiologically relevant microbial models.
SN - 2304-8158
ER -