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Peer-reviewedCC BYSTAR protocols

Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and cells.

  • Yonca Keskek Turk1
  • Ailish McCafferty-Brown2
  • Clive S McKimmie3
  1. 1School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK; Department of Medical Microbiology, Virology Unit, Faculty of Medicine, Hacettepe University, Ankara, Türkiye. Electronic address: [email protected].
  2. 2School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK; Institute for Regeneration and Repair, University of Edinburgh, Edinburgh, UK.
  3. 3School of Medicine, University of Leeds, Leeds, UK; Virus Host Interaction Team, Skin Research Centre, York Biomedical Research Institute, Hull York Medical School, University of York, York, UK. Electronic address: [email protected].
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Abstract

Here, we present an in vivo approach to study virus-vector-host interactions during Toscana virus (TOSV) infection in mouse skin. We describe preparation of viral stocks and subcutaneous TOSV inoculation into dorsal upper foot skin, either alone, with sand-fly salivary gland extract (SGE), or following sand-fly biting. We then detail tissue collection and generation of single-cell suspensions. Finally, we describe flow cytometric detection of infected cells, magnetic isolation of dermal fibroblasts, and RNA extraction for transcriptomic analysis. For complete details on the use and execution of this protocol, please refer to Keskek Turk et al.¹.

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Cite

BibTeXRIS
BibTeX
@article{turk2026protocol,
  title = {{Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and cells.}},
  author = {Yonca Keskek Turk and Ailish McCafferty-Brown and Clive S McKimmie},
  journal = {STAR protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104885},
  url = {https://europepmc.org/article/MED/42848514},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Protocol for establishing a murine Toscana virus infection model and downstream cellular analysis of skin tissue and cells.
AU  - Yonca Keskek Turk
AU  - Ailish McCafferty-Brown
AU  - Clive S McKimmie
PY  - 2026
DA  - 2026/10/08
JO  - STAR protocols
DO  - 10.1016/j.xpro.2026.104885
UR  - https://europepmc.org/article/MED/42848514
AB  - Here, we present an in vivo approach to study virus-vector-host interactions during Toscana virus (TOSV) infection in mouse skin. We describe preparation of viral stocks and subcutaneous TOSV inoculation into dorsal upper foot skin, either alone, with sand-fly salivary gland extract (SGE), or following sand-fly biting. We then detail tissue collection and generation of single-cell suspensions. Finally, we describe flow cytometric detection of infected cells, magnetic isolation of dermal fibroblasts, and RNA extraction for transcriptomic analysis. For complete details on the use and execution of this protocol, please refer to Keskek Turk et al.¹.
SN  - 2666-1667
ER  -