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Peer-reviewedCC BY-NC-ND 4.0In Vivo

Combined Teriparatide and Exercise Effects on Diabetic Skeletal Muscle Ultrastructure

  • YASUHITO ASAKA1
  • YUJI KASUKAWA1
  • KOJI NOZAKA1
  • HIROYUKI TSUCHIE1
  • YUICHI ONO1
  • MANABU AKAGAWA1
  • YUSUKE YUASA1
  • YO MORISHITA1
  • MANAMI NAKANISHI1
  • YUKI ISHIGAKI1
  • AKARI HISADA1
  • HIROKI FUJITA2,3
1 more authors
  • NAOHISA MIYAKOSHI1
  1. 1Department of Orthopedic Surgery, Akita University Graduate School of Medicine, Akita, Japan
  2. 2Department of Advanced Medical Research Promotion, Akita University Graduate School of Medicine, Akita, Japan
  3. 3Center for Physician Scientist Training, Akita University Graduate School of Medicine, Akita, Japan
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Abstract

Background/Aim: The effects of combined teriparatide (TPTD) administration and exercise therapy on diabetic skeletal muscle remain unclear. This study investigated the effects of combined TPTD and treadmill exercise on skeletal muscle and mitochondrial ultrastructure in Akita mice, a model of type 1 diabetes.

Materials and Methods: Male C57BL/6J-Ins2/J mice were randomly assigned to four groups: control, exercise therapy (EX), TPTD administration, and combined exercise plus TPTD (EXTP) (n=6 each). TPTD (80 μg/kg) was administered subcutaneously three times weekly for six weeks, and treadmill exercise was performed five days/week. Body weight, fasting blood glucose, grip strength, wet muscle weight, muscle fibre cross-sectional area (CSA), mitochondrial and sarcomere ultrastructure, and gene expression were evaluated.

Results: Baseline fasting blood glucose levels were higher in the EXTP group than in the EX and TPTD groups. The EXTP group showed a greater reduction in fasting blood glucose than the other groups and higher wet muscle weight normalised to tibial length than the control group. A-band length and A-band length normalised to tibial length were also higher in the EXTP group. Electron microscopy showed larger subsarcolemmal and intermyofibrillar mitochondrial CSA in the EXTP group. Body weight-normalised grip strength, muscle fibre CSA, myofibril CSA, mitochondrial number, aspect ratio, roundness, and most gene expression levels did not differ among groups.

Conclusion: Combined TPTD administration and exercise therapy may induce structural changes in skeletal muscle and mitochondria under diabetic conditions and may contribute to maintenance or remodelling of skeletal muscle ultrastructure and mitochondrial morphology.

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Cite

BibTeXRIS
BibTeX
@article{asaka2026combined,
  title = {{Combined Teriparatide and Exercise Effects on Diabetic Skeletal Muscle Ultrastructure}},
  author = {YASUHITO ASAKA and YUJI KASUKAWA and KOJI NOZAKA and HIROYUKI TSUCHIE and YUICHI ONO and MANABU AKAGAWA and YUSUKE YUASA and YO MORISHITA and MANAMI NAKANISHI and YUKI ISHIGAKI and AKARI HISADA and HIROKI FUJITA and NAOHISA MIYAKOSHI},
  journal = {In Vivo},
  year = {2026},
  month = oct,
  doi = {10.21873/invivo.14495},
  url = {https://doi.org/10.21873/invivo.14495},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - Combined Teriparatide and Exercise Effects on Diabetic Skeletal Muscle Ultrastructure
AU  - YASUHITO ASAKA
AU  - YUJI KASUKAWA
AU  - KOJI NOZAKA
AU  - HIROYUKI TSUCHIE
AU  - YUICHI ONO
AU  - MANABU AKAGAWA
AU  - YUSUKE YUASA
AU  - YO MORISHITA
AU  - MANAMI NAKANISHI
AU  - YUKI ISHIGAKI
AU  - AKARI HISADA
AU  - HIROKI FUJITA
AU  - NAOHISA MIYAKOSHI
PY  - 2026
DA  - 2026/10/29
JO  - In Vivo
DO  - 10.21873/invivo.14495
UR  - https://doi.org/10.21873/invivo.14495
AB  - Background/Aim: The effects of combined teriparatide (TPTD) administration and exercise therapy on diabetic skeletal muscle remain unclear. This study investigated the effects of combined TPTD and treadmill exercise on skeletal muscle and mitochondrial ultrastructure in Akita mice, a model of type 1 diabetes.  Materials and Methods: Male C57BL/6J-Ins2/J mice were randomly assigned to four groups: control, exercise therapy (EX), TPTD administration, and combined exercise plus TPTD (EXTP) (n=6 each). TPTD (80 μg/kg) was administered subcutaneously three times weekly for six weeks, and treadmill exercise was performed five days/week. Body weight, fasting blood glucose, grip strength, wet muscle weight, muscle fibre cross-sectional area (CSA), mitochondrial and sarcomere ultrastructure, and gene expression were evaluated.  Results: Baseline fasting blood glucose levels were higher in the EXTP group than in the EX and TPTD groups. The EXTP group showed a greater reduction in fasting blood glucose than the other groups and higher wet muscle weight normalised to tibial length than the control group. A-band length and A-band length normalised to tibial length were also higher in the EXTP group. Electron microscopy showed larger subsarcolemmal and intermyofibrillar mitochondrial CSA in the EXTP group. Body weight-normalised grip strength, muscle fibre CSA, myofibril CSA, mitochondrial number, aspect ratio, roundness, and most gene expression levels did not differ among groups.  Conclusion: Combined TPTD administration and exercise therapy may induce structural changes in skeletal muscle and mitochondria under diabetic conditions and may contribute to maintenance or remodelling of skeletal muscle ultrastructure and mitochondrial morphology.
SN  - 0258-851X
SN  - 1791-7549
ER  -