Influence of cavity margin location and preparation technique on microleakage of Class V composite restorations: An in vitro study.
6 affiliations
- 1Department of Prosthodontics, Nova Southeastern University College of Dental Medicine, Fort Lauderdale, Florida, USA.
- 2Department of Biomaterials, Dental Materials and Prosthodontics, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Sao Paulo, Brazil.
- 3Department of Dentistry, Lutheran University of Brazil (ULBRA), Torres, Rio Grande do Sul, Brazil.
- 4Department of Restorative Dentistry, Dental Materials Division, Piracicaba Dental School (FOP UNICAMP), Piracicaba, Sao Paulo, Brazil.
- 5Department of Restorative Sciences and Public Health Dentistry, Nova Southeastern University College of Dental Medicine, Fort Lauderdale, Florida, USA.
- 6Department of Restorative Dentistry & Prosthodontics, UTHealth School of Dentistry, Houston, Texas, USA.
Abstract
Background
The characteristics of the prepared dental substrate and the cavity preparation technique may influence the quality of the adhesive interface and the marginal sealing of composite restorations. Ultrasonic cavity preparation using Chemical Vapor Deposition (CVD) diamond tips has been proposed as a minimally invasive alternative to conventional rotary instrumentation. This study aimed to evaluate the influence of cavity preparation techniques and cavity margin location on the microleakage of Class V composite resin restorations.Materials and methods
Twenty-four sound human third molars were used to prepare 48 standardized Class V cavities located at the cemento-enamel junction. Cavities were prepared using either a conventional high-speed diamond bur (KG Sorensen®) or an ultrasonic CVD diamond tip (CVDentus®). All preparations were restored with a fifth-generation etch-and-rinse adhesive system and a microhybrid composite resin. Following storage in distilled water at 37°C for seven days, specimens were immersed in 0.5% rhodamine B solution for 24 h, sectioned longitudinally, and evaluated under stereomicroscopy (40×). Microleakage was scored using a four-point ordinal scale. Data were analyzed using the Friedman nonparametric test and multiple comparisons (α=0.05).Results
The highest microleakage scores were observed in cavities with dentin margins. Mean rank values were 3.46 for the conventional bur/dentin group, 1.69 for the conventional bur/enamel group, 3.10 for the ultrasonic CVD/dentin group, and 1.75 for the ultrasonic CVD/enamel group. Groups with dentin margins showed significantly greater microleakage than enamel margins (p<0.05), whereas no significant differences were observed between conventional and ultrasonic cavity preparation (p>0.05).Conclusions
Cavity margin location significantly influenced microleakage, whereas the cavity preparation technique did not. Dentin margins exhibited significantly greater dye penetration than enamel margins regardless of the preparation method. Ultrasonic cavity preparation using CVD diamond tips demonstrated marginal sealing comparable to conventional rotary instrumentation and may be considered a viable alternative for minimally invasive restorative procedures.
Related
- Microleakage of Class II resin composite restorations restored with conventional, bulk-fill, and short fiber-reinforced composites with or without polyethylene fiber reinforcement: a comparative in vitro study.
- Effect of different surface preparation methods on shear bond strength of aged microhybrid composite restorations
- Effect of Additional Surface Conditioning Methods and Adhesive Systems on Microleakage at Composite Repair Interfaces: An in Vitro Study
- Fracture resistance, marginal integrity, and microleakage of indirect composite and computer-aided designed–computer-aided manufactured ceramic inlays in Class II MOD cavities: An in vitro study
- Comparative evaluation of shear bond strength of composite restorations and glass-incorporated restorations with calcium silicate-based cement liner: A systematic review and meta-analysis.
BibTeX
@article{schneider2026influence,
title = {{Influence of cavity margin location and preparation technique on microleakage of Class V composite restorations: An in vitro study.}},
author = {Mauricio Schneider and Jefferson David Melo de Matos and Leonardo Galeazzi Farina and Letícia Krummenauer and Mário Alexandre Coelho Sinhoreti and Robert Dobrin and Daher Antonio Queiroz},
journal = {Journal of clinical and experimental dentistry},
year = {2026},
month = aug,
doi = {10.4317/jced.64365},
url = {https://europepmc.org/article/MED/42894844},
note = {CC BY},
}
RIS
TY - JOUR
TI - Influence of cavity margin location and preparation technique on microleakage of Class V composite restorations: An in vitro study.
AU - Mauricio Schneider
AU - Jefferson David Melo de Matos
AU - Leonardo Galeazzi Farina
AU - Letícia Krummenauer
AU - Mário Alexandre Coelho Sinhoreti
AU - Robert Dobrin
AU - Daher Antonio Queiroz
PY - 2026
DA - 2026/08/28
JO - Journal of clinical and experimental dentistry
DO - 10.4317/jced.64365
UR - https://europepmc.org/article/MED/42894844
AB - Background The characteristics of the prepared dental substrate and the cavity preparation technique may influence the quality of the adhesive interface and the marginal sealing of composite restorations. Ultrasonic cavity preparation using Chemical Vapor Deposition (CVD) diamond tips has been proposed as a minimally invasive alternative to conventional rotary instrumentation. This study aimed to evaluate the influence of cavity preparation techniques and cavity margin location on the microleakage of Class V composite resin restorations.Materials and methods Twenty-four sound human third molars were used to prepare 48 standardized Class V cavities located at the cemento-enamel junction. Cavities were prepared using either a conventional high-speed diamond bur (KG Sorensen®) or an ultrasonic CVD diamond tip (CVDentus®). All preparations were restored with a fifth-generation etch-and-rinse adhesive system and a microhybrid composite resin. Following storage in distilled water at 37°C for seven days, specimens were immersed in 0.5% rhodamine B solution for 24 h, sectioned longitudinally, and evaluated under stereomicroscopy (40×). Microleakage was scored using a four-point ordinal scale. Data were analyzed using the Friedman nonparametric test and multiple comparisons (α=0.05).Results The highest microleakage scores were observed in cavities with dentin margins. Mean rank values were 3.46 for the conventional bur/dentin group, 1.69 for the conventional bur/enamel group, 3.10 for the ultrasonic CVD/dentin group, and 1.75 for the ultrasonic CVD/enamel group. Groups with dentin margins showed significantly greater microleakage than enamel margins (p<0.05), whereas no significant differences were observed between conventional and ultrasonic cavity preparation (p>0.05).Conclusions Cavity margin location significantly influenced microleakage, whereas the cavity preparation technique did not. Dentin margins exhibited significantly greater dye penetration than enamel margins regardless of the preparation method. Ultrasonic cavity preparation using CVD diamond tips demonstrated marginal sealing comparable to conventional rotary instrumentation and may be considered a viable alternative for minimally invasive restorative procedures.
SN - 1989-5488
ER -