Robust Electrochemical Aptasensing of VEGF Based on Poly-adenine Mediated DNA Self-Assembly and Triple Labeling of Silver Nanoparticles.
4 affiliations
- 1Department of Orthopedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China.
- 2Jinan Guoke Medical Technology Development Co., Ltd., Jinan 250103, China.
- 3Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
- 4Shandong Laboratory of Advanced Biomaterials and Medical Devices in Weihai, Weihai 264200, China.
Abstract
Vascular endothelial growth factor (VEGF) is a cell-secreted signaling protein that is regarded as the biomarker and therapy target for certain diseases. In order to provide an accurate and robust sensing method, we herein construct a cost-effective electrochemical interface based on polyadenine-mediated DNA self-assembly and tetrahedral DNA (TDNA). The three-dimensional DNA nanostructure possesses multiple sites to immobilize silver nanoparticles (AgNPs) for strong electrochemical response, which also contains aptamer sequence against target VEGF. Thus, the density of AgNPs can be modulated by VEGF and the recorded stripping voltammetric signal can sensitively reflect the level of target protein. The electrode modification technique and recognition element improved the practical utility of this method. It is also challenged with human serum samples, and the results are consistent with the standard method. This sensor provides a feasible way to identify VEGF for potential clinical applications.
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BibTeX
@article{zhang2026robust,
title = {{Robust Electrochemical Aptasensing of VEGF Based on Poly-adenine Mediated DNA Self-Assembly and Triple Labeling of Silver Nanoparticles.}},
author = {Zhenming Zhang and Hua Chai and Xin Ma and Peng Miao},
journal = {ACS measurement science au},
year = {2026},
month = aug,
doi = {10.1021/acsmeasuresciau.6c00254},
url = {https://europepmc.org/article/MED/42921454},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Robust Electrochemical Aptasensing of VEGF Based on Poly-adenine Mediated DNA Self-Assembly and Triple Labeling of Silver Nanoparticles.
AU - Zhenming Zhang
AU - Hua Chai
AU - Xin Ma
AU - Peng Miao
PY - 2026
DA - 2026/08/19
JO - ACS measurement science au
DO - 10.1021/acsmeasuresciau.6c00254
UR - https://europepmc.org/article/MED/42921454
AB - Vascular endothelial growth factor (VEGF) is a cell-secreted signaling protein that is regarded as the biomarker and therapy target for certain diseases. In order to provide an accurate and robust sensing method, we herein construct a cost-effective electrochemical interface based on polyadenine-mediated DNA self-assembly and tetrahedral DNA (TDNA). The three-dimensional DNA nanostructure possesses multiple sites to immobilize silver nanoparticles (AgNPs) for strong electrochemical response, which also contains aptamer sequence against target VEGF. Thus, the density of AgNPs can be modulated by VEGF and the recorded stripping voltammetric signal can sensitively reflect the level of target protein. The electrode modification technique and recognition element improved the practical utility of this method. It is also challenged with human serum samples, and the results are consistent with the standard method. This sensor provides a feasible way to identify VEGF for potential clinical applications.
SN - 2694-250X
ER -