Biochemical and Binding Characterization of a Riboflavin Analogue Tethered to Biotin

Abstract

The binding affinities of a chimeric analog of a riboflavin derivative linked to biotin, (6- (7,8-dimethyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)hexyl 5-((3aS,4S,6aR)-2- oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate), referred to as C6-Rf-biotin-tag, to the riboflavin binding retain or streptavidin are in the M range, 1.29 {+/-} 0.277 and 3.00 {+/-} 0.459, respectively. These values suggest that C6-Rf-biotin-tag has potential applications in diagnostic assay and labelling target flavin binding proteins. The C6-Rf-biotin-tag which was characterized with respect to physical and biochemical properties retains UV/Vis spectroscopic and fluorescence behavior similar to riboflavin.

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BibTeX RIS

BibTeX

@misc{marincean2026biochemical,
  title = {{Biochemical and Binding Characterization of a Riboflavin Analogue Tethered to Biotin}},
  author = {S. Marincean and S. R. Smith and T. Branscum and A. Ratajczak and M. A. Benore},
  howpublished = {bioRxiv},
  year = {2026},
  month = aug,
  doi = {10.64898/2026.08.29.748002},
  url = {https://www.biorxiv.org/content/10.64898/2026.08.29.748002v1},
  note = {CC BY},
}

RIS

TY  - UNPB
TI  - Biochemical and Binding Characterization of a Riboflavin Analogue Tethered to Biotin
AU  - S. Marincean
AU  - S. R. Smith
AU  - T. Branscum
AU  - A. Ratajczak
AU  - M. A. Benore
PY  - 2026
DA  - 2026/08/29
PB  - bioRxiv
DO  - 10.64898/2026.08.29.748002
UR  - https://www.biorxiv.org/content/10.64898/2026.08.29.748002v1
AB  - The binding affinities of a chimeric analog of a riboflavin derivative linked to biotin, (6- (7,8-dimethyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)hexyl 5-((3aS,4S,6aR)-2- oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoate), referred to as C6-Rf-biotin-tag, to the riboflavin binding retain or streptavidin are in the M range, 1.29 {+/-} 0.277 and 3.00 {+/-} 0.459, respectively. These values suggest that C6-Rf-biotin-tag has potential applications in diagnostic assay and labelling target flavin binding proteins. The C6-Rf-biotin-tag which was characterized with respect to physical and biochemical properties retains UV/Vis spectroscopic and fluorescence behavior similar to riboflavin.
ER  -

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