Assembly and Interfacial Behavior of Recombinant Surfactant Protein-B in Detergent Micelles and Lipid Monolayers.
- 1Independent Researcher
Abstract
Although surfactant protein B (SP-B) is essential for pulmonary surfactant function, the experimental structure of mature SP-B remains undetermined, and the molecular basis of its function is unclear. Here, we characterize the structural and functional behavior of a recombinant SP-B construct (rSP-B) in DPC/SDS detergent micelles and DPPC/POPG lipid monolayers. Light scattering showed that dilute DPC/SDS (9:1, 0.2% w/v) is intrinsically heterogeneous, containing both micellar (~4 nm) and supramicellar structures (~68-400 nm), consistent with non-ideal surfactant mixing at low detergent concentration. Dialysis resulted in a dominant, reproducible mesoscale structure (~600 nm) in which rSP-B retained predominantly alpha-helical structure (~47%), whereas 200 nm filtration removed most of the protein and helical signal, suggesting that rSP-B is associated with the large assemblies, although the resulting complex sizes exceed the regime typically accessible to solution NMR. In separate detergent-only measurements, higher detergent concentrations (0.5-2% w/v) gave monomodal micelles (3.6-4.1 nm), suggesting that detergent concentration influences assembly size. In parallel, incorporation of rSP-B into DPPC/POPG monolayers stabilized the hysteretic response and attenuated cycling-induced area loss relative to protein-free films, with compressibility signatures consistent with out-of-plane deformation and reversible reorganization of collapsed material. These results inform strategies for optimizing detergent conditions for future structural studies and support a model in which rSP-B may promote retention and reversible reorganization of collapsed interfacial material, potentially through mechanically coupled reservoirs.
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BibTeX
@misc{asrat2026assembly,
title = {{Assembly and Interfacial Behavior of Recombinant Surfactant Protein-B in Detergent Micelles and Lipid Monolayers.}},
author = {T. G. Asrat},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.04.743498},
url = {https://www.biorxiv.org/content/10.64898/2026.09.04.743498v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Assembly and Interfacial Behavior of Recombinant Surfactant Protein-B in Detergent Micelles and Lipid Monolayers.
AU - T. G. Asrat
PY - 2026
DA - 2026/09/04
PB - bioRxiv
DO - 10.64898/2026.09.04.743498
UR - https://www.biorxiv.org/content/10.64898/2026.09.04.743498v1
AB - Although surfactant protein B (SP-B) is essential for pulmonary surfactant function, the experimental structure of mature SP-B remains undetermined, and the molecular basis of its function is unclear. Here, we characterize the structural and functional behavior of a recombinant SP-B construct (rSP-B) in DPC/SDS detergent micelles and DPPC/POPG lipid monolayers. Light scattering showed that dilute DPC/SDS (9:1, 0.2% w/v) is intrinsically heterogeneous, containing both micellar (~4 nm) and supramicellar structures (~68-400 nm), consistent with non-ideal surfactant mixing at low detergent concentration. Dialysis resulted in a dominant, reproducible mesoscale structure (~600 nm) in which rSP-B retained predominantly alpha-helical structure (~47%), whereas 200 nm filtration removed most of the protein and helical signal, suggesting that rSP-B is associated with the large assemblies, although the resulting complex sizes exceed the regime typically accessible to solution NMR. In separate detergent-only measurements, higher detergent concentrations (0.5-2% w/v) gave monomodal micelles (3.6-4.1 nm), suggesting that detergent concentration influences assembly size. In parallel, incorporation of rSP-B into DPPC/POPG monolayers stabilized the hysteretic response and attenuated cycling-induced area loss relative to protein-free films, with compressibility signatures consistent with out-of-plane deformation and reversible reorganization of collapsed material. These results inform strategies for optimizing detergent conditions for future structural studies and support a model in which rSP-B may promote retention and reversible reorganization of collapsed interfacial material, potentially through mechanically coupled reservoirs.
ER -