The genomic landscape of Pasteurella multocida B:2 causing haemorrhagic septicaemia in Germany.
10 affiliations
- 1Friedrich-Loeffler Institut, Institut für Bakterielle Infektionen und Zoonosen, Jena, Germany.
- 2Landeslabor Berlin-Brandenburg, Institut für Lebensmittel, Arzneimittel, Tierseuchen und Umwelt, Frankfurt (Oder), Germany.
- 3Chemisches und Veterinäruntersuchungsamt Stuttgart, Fellbach, Germany.
- 4Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit, Erlangen, Germany.
- 5Landesamt für Verbraucherschutz Sachsen-Anhalt, Halle (Saale), Germany.
- 6Landesuntersuchungsamt Rheinland-Pfalz, Koblenz, Germany.
- 7Landesbetrieb Hessisches Landeslabor (LHL), Gießen, Germany.
- 8Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen Sachsen, Leipzig, Germany.
- 9HUN-REN Veterinary Medical Research Institute, Budapest, Hungary.
- 10Friedrich-Loeffler Institut, Institut für Bakterielle Infektionen und Zoonosen, Jena, Germany. [email protected].
Abstract
Haemorrhagic septicaemia (HS) is a peracute to acute, highly fatal disease caused by Pasteurella (P.) multocida serogroups B:2 and E:2 affecting cattle and buffaloes. HS in cattle and buffaloes necessitates mandatory reporting to the World Organisation for Animal Health (WOAH; formerly OIE) due to its significant socio-economic impact on livestock production and international trade. In other ungulate species infection with P. multocida B:2 (iPmB:2) presents with a similar clinical picture. A series of HS and iPmB:2 outbreaks between 2010 and 2024 in Germany required a detailed investigation into the spatiotemporal dynamics and molecular characteristics of the circulating strains. The ability to specifically identify and analyse circulating serovars is crucial for confirming outbreaks and implementing appropriate control measures. A total of 221 Pasteurella multocida capsular type B:2 isolates associated with HS/iPmB:2 outbreaks was collected between 2010 and 2024 from dead livestock and wildlife in Germany in the context of veterinary practice and subsequently analysed using whole-genome sequencing. Isolates were characterised by capsular and lipopolysaccharide PCR-based typing, multilocus sequence typing, and high-resolution genomic comparison. Four high-resolution-typing approaches were evaluated and compared with epidemiologically defined outbreaks to identify the most suitable method for outbreak investigation. Single nucleotide polymorphism (SNP)-based analysis showed the highest discriminatory power and best agreement with known outbreak data, outperforming conventional multilocus sequence typing (MLST) and core-genome multilocus sequence typing (cgMLST) approaches. Genotyping identified eight major genetic clusters of closely related strains across multiple federal states and host species. Several outbreaks were linked by highly similar or identical genomes, suggesting both local persistence and wider geographic spread of the pathogen within Germany.
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BibTeX
@article{linde2026genomic,
title = {{The genomic landscape of Pasteurella multocida B:2 causing haemorrhagic septicaemia in Germany.}},
author = {Jörg Linde and Peter Kutzer and Jörg Rau and Reinhard Sting and Nina Kaestner and Annette Schliephake and Astrid König-Mozes and Tobias Eisenberg and Heinrich Neubauer and Johannes Solle and Rebecca Bonke and Anna Rettinger and Nicole Rudolph and Tibor Magyar and Kerstin Boll and Lisa Schneider-Bühl and Lisa D Sprague},
journal = {Veterinary research},
year = {2026},
month = oct,
doi = {10.1186/s13567-026-01868-x},
url = {https://europepmc.org/article/MED/42958594},
note = {CC BY},
}
RIS
TY - JOUR
TI - The genomic landscape of Pasteurella multocida B:2 causing haemorrhagic septicaemia in Germany.
AU - Jörg Linde
AU - Peter Kutzer
AU - Jörg Rau
AU - Reinhard Sting
AU - Nina Kaestner
AU - Annette Schliephake
AU - Astrid König-Mozes
AU - Tobias Eisenberg
AU - Heinrich Neubauer
AU - Johannes Solle
AU - Rebecca Bonke
AU - Anna Rettinger
AU - Nicole Rudolph
AU - Tibor Magyar
AU - Kerstin Boll
AU - Lisa Schneider-Bühl
AU - Lisa D Sprague
PY - 2026
DA - 2026/10/29
JO - Veterinary research
DO - 10.1186/s13567-026-01868-x
UR - https://europepmc.org/article/MED/42958594
AB - Haemorrhagic septicaemia (HS) is a peracute to acute, highly fatal disease caused by Pasteurella (P.) multocida serogroups B:2 and E:2 affecting cattle and buffaloes. HS in cattle and buffaloes necessitates mandatory reporting to the World Organisation for Animal Health (WOAH; formerly OIE) due to its significant socio-economic impact on livestock production and international trade. In other ungulate species infection with P. multocida B:2 (iPmB:2) presents with a similar clinical picture. A series of HS and iPmB:2 outbreaks between 2010 and 2024 in Germany required a detailed investigation into the spatiotemporal dynamics and molecular characteristics of the circulating strains. The ability to specifically identify and analyse circulating serovars is crucial for confirming outbreaks and implementing appropriate control measures. A total of 221 Pasteurella multocida capsular type B:2 isolates associated with HS/iPmB:2 outbreaks was collected between 2010 and 2024 from dead livestock and wildlife in Germany in the context of veterinary practice and subsequently analysed using whole-genome sequencing. Isolates were characterised by capsular and lipopolysaccharide PCR-based typing, multilocus sequence typing, and high-resolution genomic comparison. Four high-resolution-typing approaches were evaluated and compared with epidemiologically defined outbreaks to identify the most suitable method for outbreak investigation. Single nucleotide polymorphism (SNP)-based analysis showed the highest discriminatory power and best agreement with known outbreak data, outperforming conventional multilocus sequence typing (MLST) and core-genome multilocus sequence typing (cgMLST) approaches. Genotyping identified eight major genetic clusters of closely related strains across multiple federal states and host species. Several outbreaks were linked by highly similar or identical genomes, suggesting both local persistence and wider geographic spread of the pathogen within Germany.
SN - 0928-4249
SN - 1297-9716
ER -