Formulation, characterization, and efficacy evaluation of testolift: a novel testosterone-enhancing nutraceutical featuring synergistic black ginger methoxyflavones, fenugreek saponins, and zinc methionine via InSitu360 technology
4 affiliations
- 1NIMP Innovation Hub, Padmalife Nutrition Private Limited Koratty Thrissur - 680 309 Kerala India
- 2Innovation Centre, Padmalife Nutrition Private Limited, Mahatma Gandhi University Innovation Foundation Building No. 10/572, Priyadarshini Hills Athirampuzha, Kottayam Kerala 686560 India
- 3Global Innovation Centre, Molecules Biolabs Private Limited Koratty Thrissur - 680 309 Kerala India
- 4Atomin Innovations Private Limited Meloor, Thrissur – 680311 Kerala India
Abstract
Declining testosterone levels are associated with reduced muscle mass, fatigue, and reproductive challenges in men. Although testosterone replacement therapy is available, it is often associated with risks such as cardiovascular complications and endocrine imbalance, highlighting the need for safer alternatives. Nutraceutical approaches offer a promising strategy for supporting endogenous testosterone production. In this study, Testolift, a novel testosterone-supporting nutraceutical formulation, was developed under the Natural Ingredients for Mental and Performance (NIMP) Platform using InSitu360 molecular complexation technology. The formulation integrates fenugreek saponins, black ginger (Kaempferia parviflora) polymethoxyflavones, and zinc methionine to achieve synergistic enhancement of testosterone. The formulation was characterized using FT-IR, SEM, TEM, DSC, EDS, and UPLC, confirming the successful integration of bioactives and favorable physicochemical properties. Quantitative analysis verified the presence of 20% saponins, 0.93% polymethoxyflavones, and 1.4% elemental zinc. Morphological studies revealed uniformly dispersed spherical nano- and microspheres with sub-200 nm particles, supporting structural stability and efficient incorporation of bioactives within the matrix. Dynamic light scattering analysis confirmed a mean particle size of 193 nm with a distribution range of 120–260 nm, while the zeta potential of −32.24 mV indicated good colloidal stability. A 180 days stability study demonstrated >95% retention of methoxyflavones, saponins, and zinc across refrigerated (4 ± 2 °C), ambient (25 ± 2 °C), and accelerated (45 ± 2 °C) conditions, confirming excellent compositional and physicochemical stability. In a 42 days in vivo experimental study in male rats, Testolift significantly increased serum testosterone by 22.7% compared to 4.1% in controls (p < 0.05) without adverse effects. The InSitu360 technology enhanced the physicochemical and compositional stability of the formulation, supporting its robustness and suggesting potential for improved bioavailability, which warrants confirmation in future clinical studies. These results position Testolift as a promising natural testosterone-support formulation with potential applications in male health and muscle endurance.
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BibTeX
@article{amalraj2026formulation,
title = {{Formulation, characterization, and efficacy evaluation of testolift: a novel testosterone-enhancing nutraceutical featuring synergistic black ginger methoxyflavones, fenugreek saponins, and zinc methionine via InSitu360 technology}},
author = {Augustine Amalraj and Kaniyath Ramachandran Reshna and Karthik Varma and Ann Mariya Jogy and Preetha Balakrishnan and Sreerag Gopi},
journal = {RSC Advances},
year = {2026},
month = jul,
doi = {10.1039/d6ra02748b},
url = {https://doi.org/10.1039/d6ra02748b},
note = {CC BY 3.0},
}
RIS
TY - JOUR
TI - Formulation, characterization, and efficacy evaluation of testolift: a novel testosterone-enhancing nutraceutical featuring synergistic black ginger methoxyflavones, fenugreek saponins, and zinc methionine via InSitu360 technology
AU - Augustine Amalraj
AU - Kaniyath Ramachandran Reshna
AU - Karthik Varma
AU - Ann Mariya Jogy
AU - Preetha Balakrishnan
AU - Sreerag Gopi
PY - 2026
DA - 2026/07/02
JO - RSC Advances
DO - 10.1039/d6ra02748b
UR - https://doi.org/10.1039/d6ra02748b
AB - Declining testosterone levels are associated with reduced muscle mass, fatigue, and reproductive challenges in men. Although testosterone replacement therapy is available, it is often associated with risks such as cardiovascular complications and endocrine imbalance, highlighting the need for safer alternatives. Nutraceutical approaches offer a promising strategy for supporting endogenous testosterone production. In this study, Testolift, a novel testosterone-supporting nutraceutical formulation, was developed under the Natural Ingredients for Mental and Performance (NIMP) Platform using InSitu360 molecular complexation technology. The formulation integrates fenugreek saponins, black ginger (Kaempferia parviflora) polymethoxyflavones, and zinc methionine to achieve synergistic enhancement of testosterone. The formulation was characterized using FT-IR, SEM, TEM, DSC, EDS, and UPLC, confirming the successful integration of bioactives and favorable physicochemical properties. Quantitative analysis verified the presence of 20% saponins, 0.93% polymethoxyflavones, and 1.4% elemental zinc. Morphological studies revealed uniformly dispersed spherical nano- and microspheres with sub-200 nm particles, supporting structural stability and efficient incorporation of bioactives within the matrix. Dynamic light scattering analysis confirmed a mean particle size of 193 nm with a distribution range of 120–260 nm, while the zeta potential of −32.24 mV indicated good colloidal stability. A 180 days stability study demonstrated >95% retention of methoxyflavones, saponins, and zinc across refrigerated (4 ± 2 °C), ambient (25 ± 2 °C), and accelerated (45 ± 2 °C) conditions, confirming excellent compositional and physicochemical stability. In a 42 days in vivo experimental study in male rats, Testolift significantly increased serum testosterone by 22.7% compared to 4.1% in controls (p < 0.05) without adverse effects. The InSitu360 technology enhanced the physicochemical and compositional stability of the formulation, supporting its robustness and suggesting potential for improved bioavailability, which warrants confirmation in future clinical studies. These results position Testolift as a promising natural testosterone-support formulation with potential applications in male health and muscle endurance.
SN - 2046-2069
ER -