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Peer-reviewedCC BY-NC-NDNature Communications

N-glycopedia: libraries for native N-glycan structural analysis

  • Christopher Ashwood1,2,3
  • Richard D. Cummings4
4 affiliations
  1. 1Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
  2. 2Glycomics Core, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
  3. 3Protea Glycosciences Pty Ltd., Wollongong, NSW, Australia
  4. 4Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. [email protected]
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Abstract

Studies on glycans of glycoproteins are hampered by the lack of standards that reflect the wide diversity in structure typically observed. To this end we have exploited a large library of N -glycan standards comprised of a unique collection of 226 N -glycans including oligomannose, hybrid, and complex-type. We generated a method employing porous graphitized carbon (PGC) and liquid chromatography mass spectrometry (PGC-LC-MS), which can provide a high degree of resolution of underivatized N -glycan structures. Chromatogram libraries arising from these studies include retention time data, diagnostic fragments, and validated structural assignments, providing a robust platform for both targeted and discovery-based glycomics. We refer to this as an N -glycopedia, the first type of resource in which researchers can compare this collective data to N -glycans under study and overcome the limitations of only having compositional data and predicted structures. The technology is easily expandable to include additional N -glycans as new standards become available.

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Cite

BibTeXRIS
BibTeX
@article{ashwood2026nglycopedia,
  title = {{N-glycopedia: libraries for native N-glycan structural analysis}},
  author = {Christopher Ashwood and Richard D. Cummings},
  journal = {Nature Communications},
  year = {2026},
  month = may,
  doi = {10.1038/s41467-026-73091-3},
  url = {https://doi.org/10.1038/s41467-026-73091-3},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - N-glycopedia: libraries for native N-glycan structural analysis
AU  - Christopher Ashwood
AU  - Richard D. Cummings
PY  - 2026
DA  - 2026/05/20
JO  - Nature Communications
DO  - 10.1038/s41467-026-73091-3
UR  - https://doi.org/10.1038/s41467-026-73091-3
AB  - Studies on glycans of glycoproteins are hampered by the lack of standards that reflect the wide diversity in structure typically observed. To this end we have exploited a large library of N -glycan standards comprised of a unique collection of 226 N -glycans including oligomannose, hybrid, and complex-type. We generated a method employing porous graphitized carbon (PGC) and liquid chromatography mass spectrometry (PGC-LC-MS), which can provide a high degree of resolution of underivatized N -glycan structures. Chromatogram libraries arising from these studies include retention time data, diagnostic fragments, and validated structural assignments, providing a robust platform for both targeted and discovery-based glycomics. We refer to this as an N -glycopedia, the first type of resource in which researchers can compare this collective data to N -glycans under study and overcome the limitations of only having compositional data and predicted structures. The technology is easily expandable to include additional N -glycans as new standards become available.
SN  - 2041-1723
ER  -