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Peer-reviewedCC BY 4.0Biomolecules

Establishment of Immunochromatographic Test Strips for Porcine Epidemic Diarrhea Virus (PEDV) Antibody Detection Based on Monoclonal Antibodies Against S2 Protein

  • Meng Zhong1,2
  • Rongchao Liu1,2
  • Ruiying Wang1,2
  • Ye Liu1,2
  • Zichen Gao1,2
  • Haiyan Zhang1,2
  • Jianing Hu1,2
  • Qingtao Liu3
  • Bin Zhou1,2
  • Xiuli Feng1,2
  1. 1Key Laboratory of Animal Microbiology of China’s Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
  2. 2MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
  3. 3Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
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Abstract

Porcine epidemic diarrhea (PED) is an acute infectious disease caused by the porcine epidemic diarrhea virus (PEDV). Clinical manifestations include severe diarrhea, vomiting, dehydration and even death in piglets, causing significant economic losses to the global pig farming industry. Reliable on-site diagnostic tools for early PEDV detection, particularly for emerging variants, remain scarce. The spike (S) protein plays a critical role during the viral entry process, and its S2 subunit exhibits high conservation among different PEDV strains. In this study, a recombinant pColdI-S2 protein was expressed and purified. Following mouse immunization, cell fusion, and screening, two monoclonal antibodies (mAbs) targeting distinct conserved epitopes of the S2 protein were generated using hybridoma technology. The recombinant S2 protein was then conjugated with Prussian blue nanoparticles (PBNPs) to serve as a detector reagent. Staphylococcal protein A (SPA) and the anti-S2 mAb were immobilized on the test (T) line and control (C) line of the strip, respectively. Based on these components, an immunochromatographic strip was preliminarily established for detecting antibodies specific to the S2 protein of PEDV. Our findings demonstrate the potential of this assay as a reliable tool for serological surveillance of PEDV infection in swine populations, facilitate rapid clinical diagnosis and contribute to PED control strategies.

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Cite

BibTeXRIS
BibTeX
@article{zhong2026establishment,
  title = {{Establishment of Immunochromatographic Test Strips for Porcine Epidemic Diarrhea Virus (PEDV) Antibody Detection Based on Monoclonal Antibodies Against S2 Protein}},
  author = {Meng Zhong and Rongchao Liu and Ruiying Wang and Ye Liu and Zichen Gao and Haiyan Zhang and Jianing Hu and Qingtao Liu and Bin Zhou and Xiuli Feng},
  journal = {Biomolecules},
  year = {2026},
  month = nov,
  doi = {10.3390/biom16111616},
  url = {https://www.mdpi.com/2218-273X/16/11/1616},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Establishment of Immunochromatographic Test Strips for Porcine Epidemic Diarrhea Virus (PEDV) Antibody Detection Based on Monoclonal Antibodies Against S2 Protein
AU  - Meng Zhong
AU  - Rongchao Liu
AU  - Ruiying Wang
AU  - Ye Liu
AU  - Zichen Gao
AU  - Haiyan Zhang
AU  - Jianing Hu
AU  - Qingtao Liu
AU  - Bin Zhou
AU  - Xiuli Feng
PY  - 2026
DA  - 2026/11/06
JO  - Biomolecules
DO  - 10.3390/biom16111616
UR  - https://www.mdpi.com/2218-273X/16/11/1616
AB  - Porcine epidemic diarrhea (PED) is an acute infectious disease caused by the porcine epidemic diarrhea virus (PEDV). Clinical manifestations include severe diarrhea, vomiting, dehydration and even death in piglets, causing significant economic losses to the global pig farming industry. Reliable on-site diagnostic tools for early PEDV detection, particularly for emerging variants, remain scarce. The spike (S) protein plays a critical role during the viral entry process, and its S2 subunit exhibits high conservation among different PEDV strains. In this study, a recombinant pColdI-S2 protein was expressed and purified. Following mouse immunization, cell fusion, and screening, two monoclonal antibodies (mAbs) targeting distinct conserved epitopes of the S2 protein were generated using hybridoma technology. The recombinant S2 protein was then conjugated with Prussian blue nanoparticles (PBNPs) to serve as a detector reagent. Staphylococcal protein A (SPA) and the anti-S2 mAb were immobilized on the test (T) line and control (C) line of the strip, respectively. Based on these components, an immunochromatographic strip was preliminarily established for detecting antibodies specific to the S2 protein of PEDV. Our findings demonstrate the potential of this assay as a reliable tool for serological surveillance of PEDV infection in swine populations, facilitate rapid clinical diagnosis and contribute to PED control strategies.
SN  - 2218-273X
ER  -