Detection of Mucorales antigen in bronchoalveolar lavage samples using a newly developed lateral-flow device
Abstract
A murine IgG2b monoclonal antibody, named TG11, binding to an extracellular polysaccharide antigen secreted by all Mucorales fungi has been recently developed and integrated into a lateral-flow device (TG11-LFD). The aim of this study was to establish the clinical performance of TG11-LFD on bronchoalveolar lavage (BAL) fluids for the diagnosis of mucormycosis. Thirteen BAL samples from 13 patients with mucormycosis, all of which tested positive for Mucorales qPCR ( Mucor/Rhizopus [ n = 5], Lichtheimia [ n = 2], Rhizomucor [ n = 5], and Cunninghamella [ n = 1]), were used to assess the TG11-LFD. We also selected 49 BAL samples from 25 patients with other invasive fungal infections (IFI) (aspergillosis, Pneumocystis infection, candidiasis, and possible IFI) and from 20 patients without IFI for use as negative controls. The intensities of the test and control lines were recorded using a Cube reader. The diagnostic performance was assessed by analyzing the receiver operating characteristics (ROC) curve with the Jamovi software package (version 2.6.13). The area under the curve of the ROC curve was 0.739. Using a threshold value positivity ≤531 artificial units, the TG11-LFD test has a sensitivity and specificity of 76.92% and 75.51%, respectively, a positive predictive value of 45.45%, and a negative predictive value of 92.5%. In this study, we evaluated the performance of TG11-LFD on clinical samples for the first time and demonstrated its significant potential for enhancing the rapid detection of mucormycosis. Combining antigen detection with qPCR, as successfully applied in the diagnosis of aspergillosis, is likely to yield the most reliable diagnostic approach..
Related
- A developed recombinant antigen and monoclonal antibody based solid phase competitive ELISA for detection of foot and mouth disease virus serotype O specific antibodies
- Systematic Evaluation of Nasal Immune Cell Sampling and Antigen-specific T cell Detection using Cryopreserved Nasal Swabs
- Molecular detection of Rickettsia helvetica in lesion and blood samples after tick bites in Serbia: two cases with One Health implications
- Development of a centrifugation-based method for the qualitative molecular detection of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in surface water samples.
- Reproducible detection of antigen-specific T cells and Tregs via standardized and automated activation-induced marker assay workflows
BibTeX
@article{rousselot2026detection,
title = {{Detection of Mucorales antigen in bronchoalveolar lavage samples using a newly developed lateral-flow device}},
author = {Julie Rousselot and Laurence Millon and Emeline Scherer and Nathalie Bourgeois and Sebastien Imbert and Damien Dupont and Anne Debourgogne and Danièle Maubon and Anne Pauline Bellanger and Christopher Thornton},
journal = {Journal of Clinical Microbiology},
year = {2026},
month = sep,
doi = {10.1128/jcm.00226-25},
url = {https://hal.science/hal-05757350v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Detection of Mucorales antigen in bronchoalveolar lavage samples using a newly developed lateral-flow device
AU - Julie Rousselot
AU - Laurence Millon
AU - Emeline Scherer
AU - Nathalie Bourgeois
AU - Sebastien Imbert
AU - Damien Dupont
AU - Anne Debourgogne
AU - Danièle Maubon
AU - Anne Pauline Bellanger
AU - Christopher Thornton
PY - 2026
DA - 2026/09/21
JO - Journal of Clinical Microbiology
DO - 10.1128/jcm.00226-25
UR - https://hal.science/hal-05757350v1
AB - A murine IgG2b monoclonal antibody, named TG11, binding to an extracellular polysaccharide antigen secreted by all Mucorales fungi has been recently developed and integrated into a lateral-flow device (TG11-LFD). The aim of this study was to establish the clinical performance of TG11-LFD on bronchoalveolar lavage (BAL) fluids for the diagnosis of mucormycosis. Thirteen BAL samples from 13 patients with mucormycosis, all of which tested positive for Mucorales qPCR ( Mucor/Rhizopus [ n = 5], Lichtheimia [ n = 2], Rhizomucor [ n = 5], and Cunninghamella [ n = 1]), were used to assess the TG11-LFD. We also selected 49 BAL samples from 25 patients with other invasive fungal infections (IFI) (aspergillosis, Pneumocystis infection, candidiasis, and possible IFI) and from 20 patients without IFI for use as negative controls. The intensities of the test and control lines were recorded using a Cube reader. The diagnostic performance was assessed by analyzing the receiver operating characteristics (ROC) curve with the Jamovi software package (version 2.6.13). The area under the curve of the ROC curve was 0.739. Using a threshold value positivity ≤531 artificial units, the TG11-LFD test has a sensitivity and specificity of 76.92% and 75.51%, respectively, a positive predictive value of 45.45%, and a negative predictive value of 92.5%. In this study, we evaluated the performance of TG11-LFD on clinical samples for the first time and demonstrated its significant potential for enhancing the rapid detection of mucormycosis. Combining antigen detection with qPCR, as successfully applied in the diagnosis of aspergillosis, is likely to yield the most reliable diagnostic approach..
SN - 0095-1137
ER -