Skip to content
DraconiticResearch
Search Sources API
203,948 studies31 sourcesUpdated just now
Peer-reviewedCC BY 4.0International Journal of Molecular Sciences

Characterization of Umbilical Cord Mesenchymal Stromal Cells Overexpressing Klotho and In Vitro Anti-Aging Efficacy of Their Derived Extracellular Vesicles

  • Anyuan Zhang1
  • Shan Wang1
  • Jing Zhang1
  • Bianlei Yang1
  • Xiaorong Su2
  • Wenying Deng1
  • Hongxiang Wang3
  • Zhichao Chen2
  • Qiubai Li1
  1. 1Department of Rheumatology and Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  2. 2Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
  3. 3Department of Hematology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China
Open at sourceDOICite

Abstract

Age-related degeneration remains a premier biomedical challenge, frequently underpinned by cellular senescence and stem cell dysfunction. Accumulating evidence suggests that umbilical cord mesenchymal stromal cell (UC-MSC)-derived extracellular vesicles (UC-EVs) and the longevity protein Klotho hold great promise as anti-aging biotherapeutics. Here, we strategically engineered UC-MSCs to overexpress Klotho and generated functional Klotho-enriched UC-EVs (KL-EVs). Our data demonstrated that genetic modification endowed UC-MSCs with prominent osteogenic differentiation potency and angiogenesis efficacy. More importantly, KL-EVs profoundly rescued multifaceted senescent phenotypes in both adult bone marrow mesenchymal stem cells (BMSCs) and human renal tubular epithelial cells (HK-2) in vitro. In particular, KL-EVs rescued the age-related differentiation bias of adult BMSCs by robustly prompting their osteogenic differentiation capacity. Taken together, we developed a novel type of UC-MSCs and isolated their EVs enriched with Klotho protein. This strategy integrated and enhanced the anti-aging effects of both entities in vitro, offering a new perspective for counteracting cellular senescence and alleviating age-associated degenerative alterations.

Related

  1. Human umbilical cord mesenchymal stromal cells and their small extracellular vesicles modulate host responses to titanium-coated polypropylene mesh in the vaginal wall of rats
    Fangfang Ai, Chunbin Wang, Gang Wang +3
    Frontiers in Bioengineering and BiotechnologyPeer-reviewedCC BY 4.0Full textSurgery & anaesthesiaPMC
  2. Human umbilical cord mesenchymal stem cells alleviate brain injury and promote neuroregeneration via BDNF/TrkB/CREB/PI3K-Akt pathway in preterm rats with intraventricular hemorrhage
    Stem cells translational medicinePeer-reviewedCC BYFull textGeneral medicineEurope PMC
  3. Human Umbilical Cord Mesenchymal Stem Cell‐Derived Exosomes Preserve Cartilage Homeostasis and Restore Joint Function in Experimental Osteoarthritis
    Sung‐Yen Lin, Yi‐Shan Lin, Shu‐Chun Chuang +9
    The Kaohsiung Journal of Medical SciencesPeer-reviewedCC BY 4.0Full textGeneral medicinePMC
  4. GelMA‐Based Hydrogel Encapsulating Astragalus Polysaccharides and Umbilical Cord Mesenchymal Stem Cell Exosomes Promotes Full‐Thickness Skin Wound Healing
    Jingwen Tian, Xiangjing Zhao, Jingxian Xie +5
    Chronic Diseases and Translational MedicinePeer-reviewedCC BY-NC-ND 4.0Full textGeneral medicinePMCEurope PMC
  5. Three repeated intrathecal injections of allogenic umbilical cord-derived mesenchymal stromal cells in autism spectrum disorder: a Phase I clinical trial.
    Elaheh Khodadoust, Hadi Zarafshan, Masoumeh Nouri +14
    Stem cell research & therapyPeer-reviewedCC BY-NC-NDFull textHuman geneticsEurope PMCPMC

Cite

BibTeXRIS
BibTeX
@article{zhang2026characterization,
  title = {{Characterization of Umbilical Cord Mesenchymal Stromal Cells Overexpressing Klotho and In Vitro Anti-Aging Efficacy of Their Derived Extracellular Vesicles}},
  author = {Anyuan Zhang and Shan Wang and Jing Zhang and Bianlei Yang and Xiaorong Su and Wenying Deng and Hongxiang Wang and Zhichao Chen and Qiubai Li},
  journal = {International Journal of Molecular Sciences},
  year = {2026},
  month = sep,
  doi = {10.3390/ijms27188326},
  url = {https://www.mdpi.com/1422-0067/27/18/8326},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Characterization of Umbilical Cord Mesenchymal Stromal Cells Overexpressing Klotho and In Vitro Anti-Aging Efficacy of Their Derived Extracellular Vesicles
AU  - Anyuan Zhang
AU  - Shan Wang
AU  - Jing Zhang
AU  - Bianlei Yang
AU  - Xiaorong Su
AU  - Wenying Deng
AU  - Hongxiang Wang
AU  - Zhichao Chen
AU  - Qiubai Li
PY  - 2026
DA  - 2026/09/19
JO  - International Journal of Molecular Sciences
DO  - 10.3390/ijms27188326
UR  - https://www.mdpi.com/1422-0067/27/18/8326
AB  - Age-related degeneration remains a premier biomedical challenge, frequently underpinned by cellular senescence and stem cell dysfunction. Accumulating evidence suggests that umbilical cord mesenchymal stromal cell (UC-MSC)-derived extracellular vesicles (UC-EVs) and the longevity protein Klotho hold great promise as anti-aging biotherapeutics. Here, we strategically engineered UC-MSCs to overexpress Klotho and generated functional Klotho-enriched UC-EVs (KL-EVs). Our data demonstrated that genetic modification endowed UC-MSCs with prominent osteogenic differentiation potency and angiogenesis efficacy. More importantly, KL-EVs profoundly rescued multifaceted senescent phenotypes in both adult bone marrow mesenchymal stem cells (BMSCs) and human renal tubular epithelial cells (HK-2) in vitro. In particular, KL-EVs rescued the age-related differentiation bias of adult BMSCs by robustly prompting their osteogenic differentiation capacity. Taken together, we developed a novel type of UC-MSCs and isolated their EVs enriched with Klotho protein. This strategy integrated and enhanced the anti-aging effects of both entities in vitro, offering a new perspective for counteracting cellular senescence and alleviating age-associated degenerative alterations.
SN  - 1422-0067
ER  -