GelMA‐Based Hydrogel Encapsulating Astragalus Polysaccharides and Umbilical Cord Mesenchymal Stem Cell Exosomes Promotes Full‐Thickness Skin Wound Healing
- 1Guangxi Key Laboratory of Special Biomedicine, School of Medicine, Guangxi University Nanning Guangxi China
- 2Department of Obstetrics Women and Children's Hospital Affiliated to Xiamen University Xiamen Fujian China
- 3Plastic Surgery Department Xiang'an Hospital of Xiamen University Xiamen Fujian China
Abstract
Background: Wound healing involves excessive oxidative stress and cellular dysfunction, while current single therapies remain inadequate. Astragalus polysaccharides (APS) and exosomes (Exos) possess antioxidative and regenerative properties, and gelatin methacryloyl (GelMA) hydrogel provides a biocompatible platform for localized delivery. Here, we developed an APS–Exos@GelMA hydrogel to promote wound repair.
Methods: The biological effects of APS and Exos were assessed through in vitro cellular assays, while the physicochemical properties and biocompatibility of APS–Exos@GelMA were characterized. Its therapeutic efficacy was further evaluated in a full‐thickness skin defect model through macroscopic observation and histological analysis.
Results: APS–Exos treatment showed enhanced effects compared with APS or Exos alone, improving fibroblast (130.90% ± 2.98%, p < 0.05) and keratinocyte (124.50% ± 3.18%, p < 0.01) viability, promoting fibroblast migration (74.15% ± 7.72%), and reducing oxidative damage (72.11% ± 2.13%). APS–Exos@GelMA maintained favorable hydrogel properties and biocompatibility while enabling localized incorporation and application of APS and Exos. In vivo, APS–Exos@GelMA accelerated wound closure, promoted epidermal reconstruction and extracellular matrix deposition, and exhibited good biosafety.
Conclusion: The combination of APS and Exos within an appropriate concentration range demonstrated improved therapeutic potential compared with individual treatments. The APS–Exos@GelMA hydrogel served as a biocompatible localized delivery platform to facilitate tissue repair, highlighting its potential for wound‐healing applications.
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BibTeX
@article{tian2026gelmabased,
title = {{GelMA‐Based Hydrogel Encapsulating Astragalus Polysaccharides and Umbilical Cord Mesenchymal Stem Cell Exosomes Promotes Full‐Thickness Skin Wound Healing}},
author = {Jingwen Tian and Xiangjing Zhao and Jingxian Xie and Zheng Huang and Pengbo Song and Suzhuang Hong and Cailing Tong and Zhongquan Qi},
journal = {Chronic Diseases and Translational Medicine},
year = {2026},
month = oct,
doi = {10.1002/cdt3.70072},
url = {https://doi.org/10.1002/cdt3.70072},
note = {CC BY-NC-ND 4.0},
}
RIS
TY - JOUR
TI - GelMA‐Based Hydrogel Encapsulating Astragalus Polysaccharides and Umbilical Cord Mesenchymal Stem Cell Exosomes Promotes Full‐Thickness Skin Wound Healing
AU - Jingwen Tian
AU - Xiangjing Zhao
AU - Jingxian Xie
AU - Zheng Huang
AU - Pengbo Song
AU - Suzhuang Hong
AU - Cailing Tong
AU - Zhongquan Qi
PY - 2026
DA - 2026/10/26
JO - Chronic Diseases and Translational Medicine
DO - 10.1002/cdt3.70072
UR - https://doi.org/10.1002/cdt3.70072
AB - Background: Wound healing involves excessive oxidative stress and cellular dysfunction, while current single therapies remain inadequate. Astragalus polysaccharides (APS) and exosomes (Exos) possess antioxidative and regenerative properties, and gelatin methacryloyl (GelMA) hydrogel provides a biocompatible platform for localized delivery. Here, we developed an APS–Exos@GelMA hydrogel to promote wound repair. Methods: The biological effects of APS and Exos were assessed through in vitro cellular assays, while the physicochemical properties and biocompatibility of APS–Exos@GelMA were characterized. Its therapeutic efficacy was further evaluated in a full‐thickness skin defect model through macroscopic observation and histological analysis. Results: APS–Exos treatment showed enhanced effects compared with APS or Exos alone, improving fibroblast (130.90% ± 2.98%, p < 0.05) and keratinocyte (124.50% ± 3.18%, p < 0.01) viability, promoting fibroblast migration (74.15% ± 7.72%), and reducing oxidative damage (72.11% ± 2.13%). APS–Exos@GelMA maintained favorable hydrogel properties and biocompatibility while enabling localized incorporation and application of APS and Exos. In vivo, APS–Exos@GelMA accelerated wound closure, promoted epidermal reconstruction and extracellular matrix deposition, and exhibited good biosafety. Conclusion: The combination of APS and Exos within an appropriate concentration range demonstrated improved therapeutic potential compared with individual treatments. The APS–Exos@GelMA hydrogel served as a biocompatible localized delivery platform to facilitate tissue repair, highlighting its potential for wound‐healing applications.
SN - 2095-882X
SN - 2589-0514
ER -