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Peer-reviewedCC BYMicroPublication biology

A novel technique for visualizing in vivo dynamic neuronal changes during Drosophila development.

  • Natalie Rico Carvajal1
  • Chris Q Doe2,1
  • Kristen M Lee2,1
  1. 1Institute of Neuroscience, University of Oregon, Eugene, OR, United States.
  2. 2Howard Hughes Medical Institute, Chevy Chase, MD, United States.
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Abstract

A major limitation in neurobiology is the inability to precisely track in vivo neuronal morphology over time. Tandem dimer Eos (TdEOS), a fluorescent photoconvertible protein under UV light, enables visualization and quantification of morphological changes between timepoints in a single animal. We optimized this using the Drosophila Moonwalker Descending Neuron (MDN), which undergoes morphological changes and is genetically accessible. By expressing TdEOS in MDNs, we demonstrated successful in vivo photoconversion, confocal imaging, and quantification of dendrite morphology changes between two timepoints in the same animal. Ultimately, TdEOS provides a targeted, accessible tool for tracing complex neuronal morphology overtime in vivo .

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Cite

BibTeXRIS
BibTeX
@article{carvajal2026novel,
  title = {{A novel technique for visualizing in vivo dynamic neuronal changes during Drosophila development.}},
  author = {Natalie Rico Carvajal and Chris Q Doe and Kristen M Lee},
  journal = {MicroPublication biology},
  year = {2026},
  month = oct,
  doi = {10.17912/micropub.biology.002359},
  url = {https://europepmc.org/article/MED/42997125},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - A novel technique for visualizing in vivo dynamic neuronal changes during Drosophila development.
AU  - Natalie Rico Carvajal
AU  - Chris Q Doe
AU  - Kristen M Lee
PY  - 2026
DA  - 2026/10/21
JO  - MicroPublication biology
DO  - 10.17912/micropub.biology.002359
UR  - https://europepmc.org/article/MED/42997125
AB  - A major limitation in neurobiology is the inability to precisely track in vivo neuronal morphology over time. Tandem dimer Eos (TdEOS), a fluorescent photoconvertible protein under UV light, enables visualization and quantification of morphological changes between timepoints in a single animal. We optimized this using the Drosophila Moonwalker Descending Neuron (MDN), which undergoes morphological changes and is genetically accessible. By expressing TdEOS in MDNs, we demonstrated successful in vivo photoconversion, confocal imaging, and quantification of dendrite morphology changes between two timepoints in the same animal. Ultimately, TdEOS provides a targeted, accessible tool for tracing complex neuronal morphology overtime in vivo .
SN  - 2578-9430
ER  -