X-ray µ-CT contrast agents employing highly-concentrated low-viscosity layered double hydroxide nanoparticles for high-resolution imaging of vasculature.
6 affiliations
- 1Department of Materials Science, Graduate School of Engineering, Osaka Metropolitan University 1-1 Gakuen-cho, Naka-ku, Sakai Osaka 599-8531 Japan [email protected].
- 2Center for Oral, Clinical, and Translational Sciences, Faculty of Dentistry, Oral and Craniofacial Sciences, King's College London London UK [email protected].
- 3Maternal and Fetal Health Research Centre, School of Medical Sciences, University of Manchester Manchester UK.
- 4Rosalind Franklin Institute, Harwell Science and Innovation Campus, Fermi Ave Didcot OX11 0QS UK.
- 5SAGA Light Source, 8-7 Yayoigaoka Tosu Saga 841-0005 Japan.
- 6Graduate School of Advanced Science and Engineering, Hiroshima University, Higashi-Hiroshima Hiroshima 739-8527 Japan.
Abstract
Contrast agents capable of being injected and cured in situ within the vasculature are required for angiography based on X-ray microcomputed tomography (µ-CT) for pathophysiology of biopsies or postmortem. In this study, we developed a contrast agent based on highly-concentrated methanolic dispersion of NiAl-type layered double hydroxide nanoparticles (NiAl-LDH NPs) for X-ray µ-CT imaging of vasculature. We optimized the synthesis conditions of the constituent NiAl-LDH NPs, with particular attention to drying conditions and dispersion media selection. The formulated contrast agent achieved a particle concentration >30 wt%, a curing time of ∼30 min, and low viscosity (<100 mPa s). These properties enabled successful perfusion into small vessels and capillaries giving excellent X-ray attenuation following setting without tissue maceration. Consequently, this enabled reliable vessel identification and segmentation using simple intensity-based approaches giving a robust approach to accelerated tissue screening.
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BibTeX
@article{ando2026xray,
title = {{X-ray µ-CT contrast agents employing highly-concentrated low-viscosity layered double hydroxide nanoparticles for high-resolution imaging of vasculature.}},
author = {Yosuke Ando and Sota Shimizu and Gowsihan Poologasundarampillai and Paul Brownbill and Angelos Evangelinos and Michele Darrow and Avery Pennington and Achintha Kondarage and Akio Yoneyama and Naoki Tarutani and Atsushi Nakahira and Yasuaki Tokudome},
journal = {Materials advances},
year = {2026},
month = oct,
doi = {10.1039/d6ma00511j},
url = {https://europepmc.org/article/MED/42877626},
note = {CC BY},
}
RIS
TY - JOUR
TI - X-ray µ-CT contrast agents employing highly-concentrated low-viscosity layered double hydroxide nanoparticles for high-resolution imaging of vasculature.
AU - Yosuke Ando
AU - Sota Shimizu
AU - Gowsihan Poologasundarampillai
AU - Paul Brownbill
AU - Angelos Evangelinos
AU - Michele Darrow
AU - Avery Pennington
AU - Achintha Kondarage
AU - Akio Yoneyama
AU - Naoki Tarutani
AU - Atsushi Nakahira
AU - Yasuaki Tokudome
PY - 2026
DA - 2026/10/12
JO - Materials advances
DO - 10.1039/d6ma00511j
UR - https://europepmc.org/article/MED/42877626
AB - Contrast agents capable of being injected and cured in situ within the vasculature are required for angiography based on X-ray microcomputed tomography (µ-CT) for pathophysiology of biopsies or postmortem. In this study, we developed a contrast agent based on highly-concentrated methanolic dispersion of NiAl-type layered double hydroxide nanoparticles (NiAl-LDH NPs) for X-ray µ-CT imaging of vasculature. We optimized the synthesis conditions of the constituent NiAl-LDH NPs, with particular attention to drying conditions and dispersion media selection. The formulated contrast agent achieved a particle concentration >30 wt%, a curing time of ∼30 min, and low viscosity (<100 mPa s). These properties enabled successful perfusion into small vessels and capillaries giving excellent X-ray attenuation following setting without tissue maceration. Consequently, this enabled reliable vessel identification and segmentation using simple intensity-based approaches giving a robust approach to accelerated tissue screening.
SN - 2633-5409
ER -