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Peer-reviewedCC BYSTAR Protocols

Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue

  • Jennifer Ngo
  • Ilan Benador
  • Alexandra Brownstein
  • Laurent Vergnes
  • Michaela Veliova
  • Michael Shum
  • Rebeca Acín-Pérez
  • Karen Reue
  • Orian Shirihai
  • Marc Liesa
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Abstract

Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry. For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).

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Cite

BibTeXRIS
BibTeX
@article{ngo2026isolation,
  title = {{Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue}},
  author = {Jennifer Ngo and Ilan Benador and Alexandra Brownstein and Laurent Vergnes and Michaela Veliova and Michael Shum and Rebeca Acín-Pérez and Karen Reue and Orian Shirihai and Marc Liesa},
  journal = {STAR Protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2020.100243},
  url = {https://hal.science/hal-05795862v1},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue
AU  - Jennifer Ngo
AU  - Ilan Benador
AU  - Alexandra Brownstein
AU  - Laurent Vergnes
AU  - Michaela Veliova
AU  - Michael Shum
AU  - Rebeca Acín-Pérez
AU  - Karen Reue
AU  - Orian Shirihai
AU  - Marc Liesa
PY  - 2026
DA  - 2026/10/12
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2020.100243
UR  - https://hal.science/hal-05795862v1
AB  - Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry. For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).
SN  - 2666-1667
ER  -