Skip to content
DraconiticResearch
Search Sources API
203,203 studies31 sourcesUpdated 54 min ago
Peer-reviewedCC BY-NC-ND 4.0STAR Protocols

Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells

  • Ghazaleh Dadashizadeh1
  • Fadi Khalaf2
  • Stephanie Wojtowicz-Piotrowski3
  • Marc G Jeschke4
4 affiliations
  1. 1Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Center for Burn Research, Hamilton Health Sciences, Hamilton, ON, Canada; Centre for Metabolism, Obesity, and Diabetes Research, McMaster University, Hamilton, ON L8S 4L8, Canada. Electronic address: [email protected].
  2. 2Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Center for Burn Research, Hamilton Health Sciences, Hamilton, ON, Canada; Centre for Metabolism, Obesity, and Diabetes Research, McMaster University, Hamilton, ON L8S 4L8, Canada.
  3. 3Center for Burn Research, Hamilton Health Sciences, Hamilton, ON, Canada; Centre for Metabolism, Obesity, and Diabetes Research, McMaster University, Hamilton, ON L8S 4L8, Canada; Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada.
  4. 4Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Center for Burn Research, Hamilton Health Sciences, Hamilton, ON, Canada; Centre for Metabolism, Obesity, and Diabetes Research, McMaster University, Hamilton, ON L8S 4L8, Canada; Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada. Electronic address: [email protected].
Read full textPDFDOICite

Abstract

We present a protocol for isolating and culturing murine adipose progenitor cells (APCs) from adipose tissue. We describe steps for tissue dissection, unit-normalized enzymatic digestion, stromal vascular fraction (SVF) isolation, APC enrichment, and cell culture. We then detail quality control procedures that maximize cell yield, viability, and purity. This protocol combines enzymatic SVF isolation with magnetic-activated cell sorting enrichment to obtain a highly enriched APC population. It emphasizes reproducibility through unit-normalized digestion, controlled processing conditions, and standardized quality control metrics.

Related

  1. Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue
    Jennifer Ngo, Ilan Benador, Alexandra Brownstein +7
    STAR ProtocolsPeer-reviewedCC BYFull text24 citedPhysiologyHAL
  2. Rapid diagnosis, genome sequencing and culture isolation of a monkeypox virus of clade Ib from a Ugandan during a professional stay in France
    Stheme de Jubecourt Aymery, Florian Cardona, Gwilherm Penant +4
    IJID RegionsPeer-reviewedCC BY 4.0Full textMicrobiologyCardiovascular medicineHAL
  3. Extraplatelet Molecule Enrichment Enhances the Ability of Platelet-Rich Plasma Releasate to Buffer IL-1β-Induced Inflammation and Oxidative Stress in a Primary Human Chondrocyte–Synoviocyte Co-Culture In Vitro System of Osteoarthritis
    Daniel Marijuán-Pinel, Jon Mercader-Ruiz, Xabier Sansinanea +6
    International Journal of Molecular SciencesPeer-reviewedCC BY 4.0Full textGeneral medicineCrossrefEurope PMC
  4. Intraglandular treatment with adipose-derived mesenchymal stem cells in patients with xerostomia due to Sjögren’s disease—a study protocol for aphase 1, triple-blinded, randomized, clinical trial
    Joachim Hansen, Kathrine Kronberg Jakobsen, Amanda-Louise Fenger Carlander +8
    TrialsPeer-reviewedCC BY-NC-ND 4.0PhysiologyCrossref
  5. Protocol for a phase I/II, open-label, single-arm trial of autologous chemically induced liver progenitor (CLiP) cell transplantation in patients with decompensated cirrhosis
    Takashi Hamada, Yasuhiro Maruya, Kazuhiro Nagai +9
    Regenerative TherapyPeer-reviewedCC BY-NC-NDFull textCell biologyGeneral medicineDOAJEurope PMCEurope PMCPMC

Cite

BibTeXRIS
BibTeX
@article{dadashizadeh2026protocol,
  title = {{Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells}},
  author = {Ghazaleh Dadashizadeh and Fadi Khalaf and Stephanie Wojtowicz-Piotrowski and Marc G Jeschke},
  journal = {STAR Protocols},
  year = {2026},
  month = oct,
  doi = {10.1016/j.xpro.2026.104901},
  url = {https://doi.org/10.1016/j.xpro.2026.104901},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells
AU  - Ghazaleh Dadashizadeh
AU  - Fadi Khalaf
AU  - Stephanie Wojtowicz-Piotrowski
AU  - Marc G Jeschke
PY  - 2026
DA  - 2026/10/16
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2026.104901
UR  - https://doi.org/10.1016/j.xpro.2026.104901
AB  - We present a protocol for isolating and culturing murine adipose progenitor cells (APCs) from adipose tissue. We describe steps for tissue dissection, unit-normalized enzymatic digestion, stromal vascular fraction (SVF) isolation, APC enrichment, and cell culture. We then detail quality control procedures that maximize cell yield, viability, and purity. This protocol combines enzymatic SVF isolation with magnetic-activated cell sorting enrichment to obtain a highly enriched APC population. It emphasizes reproducibility through unit-normalized digestion, controlled processing conditions, and standardized quality control metrics.
SN  - 2666-1667
ER  -