A Novel Feruloyl Esterase-Producing Strain ZH-1 Belonging to Bacillus altitudinis from Baijiu Distiller's Grains and Its Enzyme Cocktail with No Detectable β-Xylosidase Activity for Producing Functional Sugars from Arabinoxylan-Type Substrates.
- 1College of Life Science, Luoyang Normal University, Luoyang 471934, China.
- 2College of Forestry, Henan Agricultural University, Zhengzhou 450046, China.
Abstract
Arabinoxylan-rich agricultural residues are promising feedstocks for the production of high-value functional sugars and ferulic acid, yet their efficient enzymatic conversion is hindered by the complex crosslinked structures. This study aimed to isolate a novel feruloyl esterase-producing strain from Baijiu distiller's grains and evaluate its enzyme cocktail for arabinoxylan degradation. A novel strain, Bacillus altitudinis ZH-1, was isolated and systematically characterized. Through one-factor-at-a-time optimization of both the medium and cultivation conditions, the feruloyl esterase activity was substantially increased (15.78-fold) compared with the activity obtained in the unoptimized Luria-Bertani (LB) medium. Genomic analysis revealed 103 unique carbohydrate-active enzyme (CAZyme)-encoding genes, including endoxylanases, α-L-arabinofuranosidases, and three putative feruloyl esterases. Studies on enzymatic properties revealed that the extracellular enzyme cocktail exhibited a hemicellulase-dominated profile and no detectable β-xylosidase activity under the assay conditions, with the three key enzymes showing over 76% of their maximum activity at pH 6.0 and 50 °C. In the degradation of insoluble wheat arabinoxylan, the enzyme cocktail released L-arabinose (69.00 mg/g substrate) and a xylooligosaccharides-equivalent fraction (244.00 mg/g substrate), which together accounted for 99.57% of total reducing sugars, along with ferulic acid (1.23 mg/g substrate). When applied to de-starched wheat bran, the enzyme cocktail achieved reducing sugar yields comparable to the commercial enzyme CTec2, while producing significantly higher levels of L-arabinose and xylooligosaccharides. These findings highlight the ZH-1 enzyme cocktail as an arabinoxylan-targeting biocatalyst with no detectable β-xylosidase activity under the assay conditions, showing promising potential for producing high-value functional sugars and ferulic acid from agricultural residues.
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BibTeX
@article{wang2026novel,
title = {{A Novel Feruloyl Esterase-Producing Strain ZH-1 Belonging to Bacillus altitudinis from Baijiu Distiller's Grains and Its Enzyme Cocktail with No Detectable β-Xylosidase Activity for Producing Functional Sugars from Arabinoxylan-Type Substrates.}},
author = {Ruonan Wang and Yi Yang and Mengyang Huang and Xiaoge Li and Xinying Wu and Zihan Zhu and Jing Yang and Kongyang Wu and Zitai Sang},
journal = {Foods (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/foods15203642},
url = {https://europepmc.org/article/MED/42940279},
note = {CC BY},
}
RIS
TY - JOUR
TI - A Novel Feruloyl Esterase-Producing Strain ZH-1 Belonging to Bacillus altitudinis from Baijiu Distiller's Grains and Its Enzyme Cocktail with No Detectable β-Xylosidase Activity for Producing Functional Sugars from Arabinoxylan-Type Substrates.
AU - Ruonan Wang
AU - Yi Yang
AU - Mengyang Huang
AU - Xiaoge Li
AU - Xinying Wu
AU - Zihan Zhu
AU - Jing Yang
AU - Kongyang Wu
AU - Zitai Sang
PY - 2026
DA - 2026/10/14
JO - Foods (Basel, Switzerland)
DO - 10.3390/foods15203642
UR - https://europepmc.org/article/MED/42940279
AB - Arabinoxylan-rich agricultural residues are promising feedstocks for the production of high-value functional sugars and ferulic acid, yet their efficient enzymatic conversion is hindered by the complex crosslinked structures. This study aimed to isolate a novel feruloyl esterase-producing strain from Baijiu distiller's grains and evaluate its enzyme cocktail for arabinoxylan degradation. A novel strain, Bacillus altitudinis ZH-1, was isolated and systematically characterized. Through one-factor-at-a-time optimization of both the medium and cultivation conditions, the feruloyl esterase activity was substantially increased (15.78-fold) compared with the activity obtained in the unoptimized Luria-Bertani (LB) medium. Genomic analysis revealed 103 unique carbohydrate-active enzyme (CAZyme)-encoding genes, including endoxylanases, α-L-arabinofuranosidases, and three putative feruloyl esterases. Studies on enzymatic properties revealed that the extracellular enzyme cocktail exhibited a hemicellulase-dominated profile and no detectable β-xylosidase activity under the assay conditions, with the three key enzymes showing over 76% of their maximum activity at pH 6.0 and 50 °C. In the degradation of insoluble wheat arabinoxylan, the enzyme cocktail released L-arabinose (69.00 mg/g substrate) and a xylooligosaccharides-equivalent fraction (244.00 mg/g substrate), which together accounted for 99.57% of total reducing sugars, along with ferulic acid (1.23 mg/g substrate). When applied to de-starched wheat bran, the enzyme cocktail achieved reducing sugar yields comparable to the commercial enzyme CTec2, while producing significantly higher levels of L-arabinose and xylooligosaccharides. These findings highlight the ZH-1 enzyme cocktail as an arabinoxylan-targeting biocatalyst with no detectable β-xylosidase activity under the assay conditions, showing promising potential for producing high-value functional sugars and ferulic acid from agricultural residues.
SN - 2304-8158
ER -