Electron microscopy reveals water networks inside hydrated cellulose fibers
- 1University of Virginia, Howard Hughes Medical Institute
Abstract
Cellulose is a highly abundant linear glucose polymer that exists in amorphous and fibrillar forms1-3. Primarily produced by vascular plants but also microbes and tunicates, cellulose predominantly performs architectural functions by stabilizing cell walls, tissues, and multicellular communities4-9. Owing to its association with other cell surface materials, physiological higher-order structures of cellulose have been difficult to obtain. Here, we describe a never-dried tunicate cellulose fibril structure by cryogenic electron microscopy, which resolves a fiber of more than 360 cellulose strands. Our data reveals linear and severely twisted fiber segments that are interspersed with solvent channels. Solid-state NMR analyses and molecular dynamics simulations indicate the presence of structural water molecules that form a network connecting neighboring cellulose chains. Confocal and super-resolution MINFLUX fluorescence imaging using cellulose-specific probes as well as biochemical analyses demonstrate shared surface and material properties of plant and tunicate cellulose fibers. Combined, our data suggest that architectural water molecules may be common features of cellulose fibers and likely other polysaccharide assemblies in their hydrated physiological states.
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BibTeX
@misc{ho2026electron,
title = {{Electron microscopy reveals water networks inside hydrated cellulose fibers}},
author = {R. Ho and A. Artherya and Y. Pankratova and C. Lim and L. F. L. Wilson and M. Hong and K. Nakashima and J. Zimmer},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.16.752152},
url = {https://www.biorxiv.org/content/10.64898/2026.09.16.752152v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Electron microscopy reveals water networks inside hydrated cellulose fibers
AU - R. Ho
AU - A. Artherya
AU - Y. Pankratova
AU - C. Lim
AU - L. F. L. Wilson
AU - M. Hong
AU - K. Nakashima
AU - J. Zimmer
PY - 2026
DA - 2026/09/16
PB - bioRxiv
DO - 10.64898/2026.09.16.752152
UR - https://www.biorxiv.org/content/10.64898/2026.09.16.752152v1
AB - Cellulose is a highly abundant linear glucose polymer that exists in amorphous and fibrillar forms1-3. Primarily produced by vascular plants but also microbes and tunicates, cellulose predominantly performs architectural functions by stabilizing cell walls, tissues, and multicellular communities4-9. Owing to its association with other cell surface materials, physiological higher-order structures of cellulose have been difficult to obtain. Here, we describe a never-dried tunicate cellulose fibril structure by cryogenic electron microscopy, which resolves a fiber of more than 360 cellulose strands. Our data reveals linear and severely twisted fiber segments that are interspersed with solvent channels. Solid-state NMR analyses and molecular dynamics simulations indicate the presence of structural water molecules that form a network connecting neighboring cellulose chains. Confocal and super-resolution MINFLUX fluorescence imaging using cellulose-specific probes as well as biochemical analyses demonstrate shared surface and material properties of plant and tunicate cellulose fibers. Combined, our data suggest that architectural water molecules may be common features of cellulose fibers and likely other polysaccharide assemblies in their hydrated physiological states.
ER -