Screening of endophytic bacteria of Leucojum aestivum ‘Gravety giant’ as a potential source of alkaloids and as antagonist to some plant fungal pathogens
Abstract
Leucojum aestivum is a medicinal plant belonging to the Amaryllidaceae family well known as a producer of alkaloids such as galanthamine and lycorine. However, the endophytic microbes that colonize different plant tissues without causing any damage have not been reported in this plant. Here, we explored the different endophytic bacterial communities isolated from different surface disinfected tissues of L. aestivum ‘Gravety giant’ and screened bacterial isolates producing alkaloids and their potential use as biocontrol agent against wheat pathogens. For that purpose, endophytic bacteria were isolated from bulbs, roots and shoots of L. aestivum. After taxonomical characterization, these microorganisms were screened for their ability to produce alkaloids using high-performance thin-layer chromatography (HPTLC) and untargeted liquid chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) strategies. We isolated 138 bacteria belonging to four phyla and 42 genera, mainly from roots and shoots. The most abundant genera were Rahnella in shoot, Patulibacter in bulb and Bacillus in roots. Among the different bacterial isolates, the methanolic extracts of Luteibacter rhizovicinus (LaBFB3301) and Commamonas denitrificans (LaBFS2103) slightly delayed the growth of F. graminearum colonies in in vitro dual tests against F. graminearum and M. nivale strains with 15.5% and 19.9% inhibition rates, respectively. These isolates are able to produce an indolic alkaloid tryptophol (C10H11NO, [M + H]+ 162.0913). These endophytic bacteria might be investigated to characterize the plant protection effect and the plant growth promotion effect.
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BibTeX
@article{yuka2026screening,
title = {{Screening of endophytic bacteria of Leucojum aestivum ‘Gravety giant’ as a potential source of alkaloids and as antagonist to some plant fungal pathogens}},
author = {Munakata Yuka and Rosella Spina and Sophie Slezack-Deschaumes and Julie Genestier and Alain Hehn and Dominique Laurain-Mattar},
journal = {Microorganisms},
year = {2026},
month = sep,
doi = {10.3390/microorganisms10102089},
url = {https://hal.univ-lorraine.fr/hal-03889320v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Screening of endophytic bacteria of Leucojum aestivum ‘Gravety giant’ as a potential source of alkaloids and as antagonist to some plant fungal pathogens
AU - Munakata Yuka
AU - Rosella Spina
AU - Sophie Slezack-Deschaumes
AU - Julie Genestier
AU - Alain Hehn
AU - Dominique Laurain-Mattar
PY - 2026
DA - 2026/09/08
JO - Microorganisms
DO - 10.3390/microorganisms10102089
UR - https://hal.univ-lorraine.fr/hal-03889320v1
AB - Leucojum aestivum is a medicinal plant belonging to the Amaryllidaceae family well known as a producer of alkaloids such as galanthamine and lycorine. However, the endophytic microbes that colonize different plant tissues without causing any damage have not been reported in this plant. Here, we explored the different endophytic bacterial communities isolated from different surface disinfected tissues of L. aestivum ‘Gravety giant’ and screened bacterial isolates producing alkaloids and their potential use as biocontrol agent against wheat pathogens. For that purpose, endophytic bacteria were isolated from bulbs, roots and shoots of L. aestivum. After taxonomical characterization, these microorganisms were screened for their ability to produce alkaloids using high-performance thin-layer chromatography (HPTLC) and untargeted liquid chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) strategies. We isolated 138 bacteria belonging to four phyla and 42 genera, mainly from roots and shoots. The most abundant genera were Rahnella in shoot, Patulibacter in bulb and Bacillus in roots. Among the different bacterial isolates, the methanolic extracts of Luteibacter rhizovicinus (LaBFB3301) and Commamonas denitrificans (LaBFS2103) slightly delayed the growth of F. graminearum colonies in in vitro dual tests against F. graminearum and M. nivale strains with 15.5% and 19.9% inhibition rates, respectively. These isolates are able to produce an indolic alkaloid tryptophol (C10H11NO, [M + H]+ 162.0913). These endophytic bacteria might be investigated to characterize the plant protection effect and the plant growth promotion effect.
SN - 2076-2607
ER -