Gene functions determine stochastic or adaptive futile transcription in cancers through deregulated start site deployments.
- 1Department of Biological Sciences and Engineering, HoMeCell Lab, Indian Institute of Technology Gandhinagar, Gandhinagar, India.
Abstract
Cancers display near-universal hallmarks, including energy addiction, aerobic glycolysis, and biosynthesis. In addition to glucose (and glutamine) addiction, cancers display futile transcription. Discretely measurable futile transcription occurs between transcription start sites (TSSs) and start codons of protein-coding genes. Multiple TSSs for each gene offer various combinations of futile transcription with no effect on the encoded proteins. The relationship between proximal and distal TSS deployment, futile transcription, and energy addiction of cancers remains unclear. By analyzing CAGE and PRO-cap data, we show that TSS deployment dysregulation in cancers increases the energy cost coefficients of cancer transcriptomes. We define the bases of the altered energy cost coefficients of cancer transcriptomes by comparing TSS deployment frequencies, associated entropies, and futile transcription distances across heterogeneous pools of cancers and non-cancers. We show that TSS deployment entropies differ between cancers and non-cancers. It leads to an overall higher distal TSS deployment in cancers but selectively favors an energy-economical proximal TSS deployment for cancer hallmark genes involved in cell proliferation and biosynthesis. We show that the frequency of TSS deployment is linked differently to futile transcription distance and gene function in cancers and non-cancers. This work lays out a theoretical framework describing the stochasticity of TSS deployment in the context of cancerogenesis and the energetics of transcription. It also suggests that the current human TSS landscape has evolved to minimize futile transcription, an outcome favored by stochastic TSS deployment. Under normal conditions of energy metabolism, it forms a threshold that incipient cancer cells breach.
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BibTeX
@article{satish2026gene,
title = {{Gene functions determine stochastic or adaptive futile transcription in cancers through deregulated start site deployments.}},
author = {Aditi Lakshmi Satish and Praveen Kumar and Ishani Morbia and Umashankar Singh},
journal = {Frontiers in bioinformatics},
year = {2026},
month = oct,
doi = {10.3389/fbinf.2026.1883461},
url = {https://europepmc.org/article/MED/42952924},
note = {CC BY},
}
RIS
TY - JOUR
TI - Gene functions determine stochastic or adaptive futile transcription in cancers through deregulated start site deployments.
AU - Aditi Lakshmi Satish
AU - Praveen Kumar
AU - Ishani Morbia
AU - Umashankar Singh
PY - 2026
DA - 2026/10/14
JO - Frontiers in bioinformatics
DO - 10.3389/fbinf.2026.1883461
UR - https://europepmc.org/article/MED/42952924
AB - Cancers display near-universal hallmarks, including energy addiction, aerobic glycolysis, and biosynthesis. In addition to glucose (and glutamine) addiction, cancers display futile transcription. Discretely measurable futile transcription occurs between transcription start sites (TSSs) and start codons of protein-coding genes. Multiple TSSs for each gene offer various combinations of futile transcription with no effect on the encoded proteins. The relationship between proximal and distal TSS deployment, futile transcription, and energy addiction of cancers remains unclear. By analyzing CAGE and PRO-cap data, we show that TSS deployment dysregulation in cancers increases the energy cost coefficients of cancer transcriptomes. We define the bases of the altered energy cost coefficients of cancer transcriptomes by comparing TSS deployment frequencies, associated entropies, and futile transcription distances across heterogeneous pools of cancers and non-cancers. We show that TSS deployment entropies differ between cancers and non-cancers. It leads to an overall higher distal TSS deployment in cancers but selectively favors an energy-economical proximal TSS deployment for cancer hallmark genes involved in cell proliferation and biosynthesis. We show that the frequency of TSS deployment is linked differently to futile transcription distance and gene function in cancers and non-cancers. This work lays out a theoretical framework describing the stochasticity of TSS deployment in the context of cancerogenesis and the energetics of transcription. It also suggests that the current human TSS landscape has evolved to minimize futile transcription, an outcome favored by stochastic TSS deployment. Under normal conditions of energy metabolism, it forms a threshold that incipient cancer cells breach.
SN - 2673-7647
ER -