Skip to content
DraconiticResearch
Search Sources API
203,234 studies31 sourcesUpdated 11 min ago
PreprintCC BYbioRxiv

Coupling Raman Spectroscopy and Acoustic Microscopy to Characterize Glioblastoma Resistance

  • T. Pesen1
  • A. Inanc
  • D. Inci
  • A. Cingoz
  • B. Akgun
  1. 1Bogazici University
Read full textPDFDOICite

Abstract

Therapy resistance in glioblastoma is usually described in genetic or metabolic terms. Here we ask whether it also has a measurable biophysical form. Combining Raman spectroscopy with scanning acoustic microscopy on TRAIL-sensitive and TRAIL-resistant glioblastoma cells, we find that resistance carries both a biochemical and a mechanical signature: the resistant cells differ at protein, lipid and nucleic-acid bands, contain 4.1-fold more neutral lipid, and hold a high acoustic impedance that does not change when the drug is applied, while sensitive cells stiffen as they die. Because acoustic impedance is an absolute quantity and neither measurement requires a label, the approach offers a route to recognising resistant populations from their physical state alone.

Related

  1. Raman and AFM-IR Spectroscopy in the Characterization of Giant Plasma Membrane Vesicles Isolated from Microglia and Glioblastoma Cells.
    Jagoda Orleańska-Pietras, Katarzyna Pogoda
    Langmuir : the ACS journal of surfaces and colloidsPeer-reviewedCC BYFull textBiophysicsEurope PMC
  2. Genetically Incorporable, Tryptophan-Based Cyan Fluorescent Amino Acid for Protein Spectroscopy and Microscopy.
    Zheyu Song, Leiting Wei, Yuyao Yang +4
    JACS AuPeer-reviewedCC BY-NC-NDFull textBiophysicsEurope PMCPMC
  3. siRNA/mRNA payload of lipid nanoparticles quantified using fluorescence microscopy and correlation spectroscopy
    B. R. Kirchmair, N. Stoyanov, D. Maksutova +6
    bioRxivPreprintCC BYFull textBiophysicsbioRxiv
  4. Translated Circular RNAs in Glioblastoma: Emerging Regulators of Tumor Progression, Molecular Heterogeneity and Therapeutic Resistance
    Robert N. Nishimura, Joseph F. Gera
    International Journal of Molecular SciencesPeer-reviewedCC BY 4.0Full textMolecular biology & gene regulationCrossrefEurope PMC
  5. Vibrational spectroscopy and artificial intelligence for dynamic phenotypic characterization of antimicrobial resistance
    Claudia P. Barrera Patiño, Jennifer M. Soares, Kate C. Blanco +1
    Frontiers in Cellular and Infection MicrobiologyPeer-reviewedCC BY 4.0Full textBiophysicsPMC

Cite

BibTeXRIS
BibTeX
@misc{pesen2026coupling,
  title = {{Coupling Raman Spectroscopy and Acoustic Microscopy to Characterize Glioblastoma Resistance}},
  author = {T. Pesen and A. Inanc and D. Inci and A. Cingoz and B. Akgun},
  howpublished = {bioRxiv},
  year = {2026},
  month = oct,
  doi = {10.64898/2026.10.14.759182},
  url = {https://www.biorxiv.org/content/10.64898/2026.10.14.759182v1},
  note = {CC BY},
}
RIS
TY  - UNPB
TI  - Coupling Raman Spectroscopy and Acoustic Microscopy to Characterize Glioblastoma Resistance
AU  - T. Pesen
AU  - A. Inanc
AU  - D. Inci
AU  - A. Cingoz
AU  - B. Akgun
PY  - 2026
DA  - 2026/10/14
PB  - bioRxiv
DO  - 10.64898/2026.10.14.759182
UR  - https://www.biorxiv.org/content/10.64898/2026.10.14.759182v1
AB  - Therapy resistance in glioblastoma is usually described in genetic or metabolic terms. Here we ask whether it also has a measurable biophysical form. Combining Raman spectroscopy with scanning acoustic microscopy on TRAIL-sensitive and TRAIL-resistant glioblastoma cells, we find that resistance carries both a biochemical and a mechanical signature: the resistant cells differ at protein, lipid and nucleic-acid bands, contain 4.1-fold more neutral lipid, and hold a high acoustic impedance that does not change when the drug is applied, while sensitive cells stiffen as they die. Because acoustic impedance is an absolute quantity and neither measurement requires a label, the approach offers a route to recognising resistant populations from their physical state alone.
ER  -