Individual mouse mitotic chromosomes exhibit cell type-specific differences in biomechanical properties
- 1Imperial College London
Abstract
Cyclic episodes of chromosome compaction and de-condensation are features of eukaryotic cell division that aid mitotic segregation and help prevent aneuploidy. While biophysical data on mitotic chromosome structure has been previously obtained, heterogeneity within samples can confound analyses and precludes direct like-for-like comparisons. To circumvent this, we employed advanced flow cytometry to purify specific metaphase chromosomes with biotinylated telomeres from stably engineered mouse cells. We show that ESC-derived metaphase chromosomes 3 and 19 display distinct properties but share a conserved force-dependent mechanical response. In contrast, chromosome equivalents isolated from NSCs and preB cells show markedly different force-dependent responses, reflecting progressive differentiation stages. Covalent crosslinking of ESC-derived chromosomes alters biomechanical properties to mimic equivalents from more differentiated cells. Collectively, these results highlight the need to isolate specific, homogeneous metaphase chromosome samples to accurately decipher their complex behaviours.
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BibTeX
@misc{brown2026individual,
title = {{Individual mouse mitotic chromosomes exhibit cell type-specific differences in biomechanical properties}},
author = {K. E. Brown and S. Patra and A. Dimond and K. K. Ray and S. Cheriyamkunnel and B. Patel and D. H. Gim and C. Whilding and M. Llobet Ayala and M. E. Kilic and D. S. Rueda and A. G. Fisher},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.07.749823},
url = {https://www.biorxiv.org/content/10.64898/2026.09.07.749823v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Individual mouse mitotic chromosomes exhibit cell type-specific differences in biomechanical properties
AU - K. E. Brown
AU - S. Patra
AU - A. Dimond
AU - K. K. Ray
AU - S. Cheriyamkunnel
AU - B. Patel
AU - D. H. Gim
AU - C. Whilding
AU - M. Llobet Ayala
AU - M. E. Kilic
AU - D. S. Rueda
AU - A. G. Fisher
PY - 2026
DA - 2026/09/07
PB - bioRxiv
DO - 10.64898/2026.09.07.749823
UR - https://www.biorxiv.org/content/10.64898/2026.09.07.749823v1
AB - Cyclic episodes of chromosome compaction and de-condensation are features of eukaryotic cell division that aid mitotic segregation and help prevent aneuploidy. While biophysical data on mitotic chromosome structure has been previously obtained, heterogeneity within samples can confound analyses and precludes direct like-for-like comparisons. To circumvent this, we employed advanced flow cytometry to purify specific metaphase chromosomes with biotinylated telomeres from stably engineered mouse cells. We show that ESC-derived metaphase chromosomes 3 and 19 display distinct properties but share a conserved force-dependent mechanical response. In contrast, chromosome equivalents isolated from NSCs and preB cells show markedly different force-dependent responses, reflecting progressive differentiation stages. Covalent crosslinking of ESC-derived chromosomes alters biomechanical properties to mimic equivalents from more differentiated cells. Collectively, these results highlight the need to isolate specific, homogeneous metaphase chromosome samples to accurately decipher their complex behaviours.
ER -