Correlation-resolved sum-frequency generation microscopy of biological tissue with bright twin beams
- 1Graduate School of Science and Technology, Keio University; Faculty of Science and Technology, Keio University
Abstract
Sum-frequency generation (SFG) driven by bright twin beams generated through high-gain parametric down-conversion comprises a coherent component arising from correlated mode pairs and an incoherent component arising from interactions between uncorrelated modes. Modal correlations can enhance coherent SFG in anisotropic nonlinear media, for example by extending the effective interaction length. Whether such correlations can likewise benefit image formation in heterogeneous biological tissue remains unknown. Here we address this question by performing SFG imaging of biological tissue using bright twin beams as the excitation source. Spectral decomposition of the SFG signal separates its coherent and incoherent components, allowing us to reconstruct co-registered images and examine how twin-beam correlations influence image formation. In mouse skeletal muscle, the coherent-component image revealed sarcomere periodicity with a Bragg-spot-to-background ratio of approximately 310, compared with 7.5 in the incoherent-component image. In fresh human dermis, the coherent-component image showed greater Fourier power than the incoherent-component image in the 0.30-0.50 \mu m^{-1} band and a greater fractional autocovariance decay at short spatial lags. These results demonstrate that twin-beam correlations can enhance contrast and detectability at selected spatial frequencies in heterogeneous tissue. Correlation-resolved SFG microscopy thus offers a route for exploiting bright quantum-light sources in nonlinear optical bioimaging.
Related
- Introducing higher harmonic generation microscopy for renal tumour pathology: a perspective on clinical translation.
- Epitope Generation Gateway: A biological context-dependent approach to cancer vaccine development
- Multivalent Adhesive Probe Atomic Force Microscopy (MAPA) for accessing dispersive adhesion of cells and biosurfaces.
- MUFASA: a continuous-time stochastic framework for realistic fluorescence microscopy simulation
- A unified framework for sensitivity comparison across stimulated Raman, stimulated Raman photothermal, and mid-infrared photothermal microscopy
BibTeX
@misc{chen2026correlationresolved,
title = {{Correlation-resolved sum-frequency generation microscopy of biological tissue with bright twin beams}},
author = {T.-H. Chen and M. Adachi and Z. Hu and M. Masaki and Y. Nikaido and R. Suzuki and M. Nuriya and Y. Eto and H. Kano},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.25.761624},
url = {https://www.biorxiv.org/content/10.64898/2026.10.25.761624v1},
note = {CC BY-NC-ND},
}
RIS
TY - UNPB
TI - Correlation-resolved sum-frequency generation microscopy of biological tissue with bright twin beams
AU - T.-H. Chen
AU - M. Adachi
AU - Z. Hu
AU - M. Masaki
AU - Y. Nikaido
AU - R. Suzuki
AU - M. Nuriya
AU - Y. Eto
AU - H. Kano
PY - 2026
DA - 2026/10/25
PB - bioRxiv
DO - 10.64898/2026.10.25.761624
UR - https://www.biorxiv.org/content/10.64898/2026.10.25.761624v1
AB - Sum-frequency generation (SFG) driven by bright twin beams generated through high-gain parametric down-conversion comprises a coherent component arising from correlated mode pairs and an incoherent component arising from interactions between uncorrelated modes. Modal correlations can enhance coherent SFG in anisotropic nonlinear media, for example by extending the effective interaction length. Whether such correlations can likewise benefit image formation in heterogeneous biological tissue remains unknown. Here we address this question by performing SFG imaging of biological tissue using bright twin beams as the excitation source. Spectral decomposition of the SFG signal separates its coherent and incoherent components, allowing us to reconstruct co-registered images and examine how twin-beam correlations influence image formation. In mouse skeletal muscle, the coherent-component image revealed sarcomere periodicity with a Bragg-spot-to-background ratio of approximately 310, compared with 7.5 in the incoherent-component image. In fresh human dermis, the coherent-component image showed greater Fourier power than the incoherent-component image in the 0.30-0.50 \mu m^{-1} band and a greater fractional autocovariance decay at short spatial lags. These results demonstrate that twin-beam correlations can enhance contrast and detectability at selected spatial frequencies in heterogeneous tissue. Correlation-resolved SFG microscopy thus offers a route for exploiting bright quantum-light sources in nonlinear optical bioimaging.
ER -