Integrated Chemical Profiling and Toxicological Assessment of Total Polyphenols from Cydonia oblonga Mill. Reveals Potential Safety Concerns Under High-Dose Exposure.
6 affiliations
- 1Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China.
- 2Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China.
- 3Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China.
- 4Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China.
- 5School of Brain Science and Brain Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China.
- 6Xinjiang Institute of Materia Medica, Urumqi 830011, China.
Abstract
The increasing consumption of polyphenol-enriched functional foods and dietary supplements highlights the importance of systematic safety evaluation of these bioactive ingredients under high-dose exposure conditions. This study comprehensively evaluated the toxicological responses associated with total polyphenols from Cydonia oblonga Mill. (TPCOM) and explored their potential biological mechanisms. Ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry identified 89 selected phenolic and related constituents. Acute and 28-day repeated-dose oral toxicity studies were conducted with reference to OECD Guideline 423 and in accordance with OECD Guideline 407, respectively. High-dose TPCOM exposure resulted in dose-associated biochemical and histopathological alterations, with the liver and kidneys identified as major target organs. Integrated systems toxicology approaches, including network toxicology, molecular docking, molecular dynamics simulations, and untargeted plasma metabolomics, revealed potential molecular targets, metabolic disturbances, and candidate biomarkers associated with TPCOM exposure. These findings suggest that excessive intake of TPCOM may be associated with metabolic imbalance, stress-related signaling alterations, and organ injury responses. Further experimental validation is required to confirm the specific molecular pathways involved. This study provides a systematic framework for evaluating the safety of polyphenol-rich functional food ingredients under high-dose exposure conditions.
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BibTeX
@article{aikebaier2026integrated,
title = {{Integrated Chemical Profiling and Toxicological Assessment of Total Polyphenols from Cydonia oblonga Mill. Reveals Potential Safety Concerns Under High-Dose Exposure.}},
author = {Alifeiye Aikebaier and Alhar Baishan and Kayisaier Abudurousuli and Dilihuma Dilimulati and Yipaerguli Paerhati and Kuerbannisha Maitikasimu and Yilixiati Wusiman and Nazhakaiti Yusufujiang and Xiaoxiao Qiu and Wenting Zhou},
journal = {Foods (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/foods15203737},
url = {https://europepmc.org/article/MED/42940378},
note = {CC BY},
}
RIS
TY - JOUR
TI - Integrated Chemical Profiling and Toxicological Assessment of Total Polyphenols from Cydonia oblonga Mill. Reveals Potential Safety Concerns Under High-Dose Exposure.
AU - Alifeiye Aikebaier
AU - Alhar Baishan
AU - Kayisaier Abudurousuli
AU - Dilihuma Dilimulati
AU - Yipaerguli Paerhati
AU - Kuerbannisha Maitikasimu
AU - Yilixiati Wusiman
AU - Nazhakaiti Yusufujiang
AU - Xiaoxiao Qiu
AU - Wenting Zhou
PY - 2026
DA - 2026/10/20
JO - Foods (Basel, Switzerland)
DO - 10.3390/foods15203737
UR - https://europepmc.org/article/MED/42940378
AB - The increasing consumption of polyphenol-enriched functional foods and dietary supplements highlights the importance of systematic safety evaluation of these bioactive ingredients under high-dose exposure conditions. This study comprehensively evaluated the toxicological responses associated with total polyphenols from Cydonia oblonga Mill. (TPCOM) and explored their potential biological mechanisms. Ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry identified 89 selected phenolic and related constituents. Acute and 28-day repeated-dose oral toxicity studies were conducted with reference to OECD Guideline 423 and in accordance with OECD Guideline 407, respectively. High-dose TPCOM exposure resulted in dose-associated biochemical and histopathological alterations, with the liver and kidneys identified as major target organs. Integrated systems toxicology approaches, including network toxicology, molecular docking, molecular dynamics simulations, and untargeted plasma metabolomics, revealed potential molecular targets, metabolic disturbances, and candidate biomarkers associated with TPCOM exposure. These findings suggest that excessive intake of TPCOM may be associated with metabolic imbalance, stress-related signaling alterations, and organ injury responses. Further experimental validation is required to confirm the specific molecular pathways involved. This study provides a systematic framework for evaluating the safety of polyphenol-rich functional food ingredients under high-dose exposure conditions.
SN - 2304-8158
ER -