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Peer-reviewedCC BYPLOS One

Nomenclature, classification, and in-silico structural analysis of MSP1a variants of Anaplasma marginale

  • Amam Zonaed Siddiki1
  • Nazifa Humaira2
  • Nanziba Sharmin Hossain2
  • Yusra Bint Akbar2
  • Puja Palit2
  • Pann Ei Phwe2
  • Rahima Akter Eva2
  • Hala Alshaar2
  • Marjia Islam Tia2
  • Kethain Marma2
  • Barsha Akter2
  • Samira Sultana2
1 more authors
  • Sabreena Alam1,3
  1. 1Genomics Research Group, Department of Pathology and Parasitology, Chittagong Veterinary and Animal Sciences University (CVASU), Chittagong, Bangladesh.
  2. 2Department of Bioinformatics and Biotechnology, Asian University of Women (AUW), Chittagong, Bangladesh.
  3. 3Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC), Chittagong, Bangladesh.
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Abstract

Bovine anaplasmosis, caused by the tick-borne rickettsial pathogen Anaplasma marginale, is a significant threat to livestock health and productivity. The major surface protein 1a (MSP1a) is a widely used molecular marker for characterizing strain diversity, due to its variable N-terminal tandem repeats. In this study, we analyzed and updated the nomenclature classification of 157 A. marginale MSP1a sequences submitted to NCBI between 2013 and 2023, following the research by Cabezas-Cruz in 2013. We identified and classified 189 unique tandem repeats, reported their tick-transmissibility, predicted their secondary structures and B-cell epitopes, and analyzed amino acid variability. Following this, we conducted a comparative analysis with previously published datasets, which revealed new tandem repeat types, new positions of serine within tandem repeats, and high variation among B-cell epitope variants. We have also classified the unique R1 tandem repeats of the new MSP1a sequences into different ecoregion clusters based on the principal component analysis of NDVI values. This updated classification system enhances the molecular understanding of A. marginale diversity and may contribute to future developments in strain typing, epidemiological investigations, and vaccine research.

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Cite

BibTeXRIS
BibTeX
@article{siddiki2026nomenclature,
  title = {{Nomenclature, classification, and in-silico structural analysis of MSP1a variants of Anaplasma marginale}},
  author = {Amam Zonaed Siddiki and Nazifa Humaira and Nanziba Sharmin Hossain and Yusra Bint Akbar and Puja Palit and Pann Ei Phwe and Rahima Akter Eva and Hala Alshaar and Marjia Islam Tia and Kethain Marma and Barsha Akter and Samira Sultana and Sabreena Alam},
  journal = {PLOS One},
  year = {2026},
  month = oct,
  doi = {10.1371/journal.pone.0360505},
  url = {https://doi.org/10.1371/journal.pone.0360505},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Nomenclature, classification, and in-silico structural analysis of MSP1a variants of Anaplasma marginale
AU  - Amam Zonaed Siddiki
AU  - Nazifa Humaira
AU  - Nanziba Sharmin Hossain
AU  - Yusra Bint Akbar
AU  - Puja Palit
AU  - Pann Ei Phwe
AU  - Rahima Akter Eva
AU  - Hala Alshaar
AU  - Marjia Islam Tia
AU  - Kethain Marma
AU  - Barsha Akter
AU  - Samira Sultana
AU  - Sabreena Alam
PY  - 2026
DA  - 2026/10/21
JO  - PLOS One
DO  - 10.1371/journal.pone.0360505
UR  - https://doi.org/10.1371/journal.pone.0360505
AB  - Bovine anaplasmosis, caused by the tick-borne rickettsial pathogen Anaplasma marginale, is a significant threat to livestock health and productivity. The major surface protein 1a (MSP1a) is a widely used molecular marker for characterizing strain diversity, due to its variable N-terminal tandem repeats. In this study, we analyzed and updated the nomenclature classification of 157 A. marginale MSP1a sequences submitted to NCBI between 2013 and 2023, following the research by Cabezas-Cruz in 2013. We identified and classified 189 unique tandem repeats, reported their tick-transmissibility, predicted their secondary structures and B-cell epitopes, and analyzed amino acid variability. Following this, we conducted a comparative analysis with previously published datasets, which revealed new tandem repeat types, new positions of serine within tandem repeats, and high variation among B-cell epitope variants. We have also classified the unique R1 tandem repeats of the new MSP1a sequences into different ecoregion clusters based on the principal component analysis of NDVI values. This updated classification system enhances the molecular understanding of A. marginale diversity and may contribute to future developments in strain typing, epidemiological investigations, and vaccine research.
SN  - 1932-6203
ER  -