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Peer-reviewedCC BY-NC 3.0RSC Advances

Europium nanoparticle-based lateral flow assay for rapid detection of herpes simplex virus 2-specific antibodies in serum: a preliminary evaluation

  • Balaji Srinivasan1
  • Julia L. Finkelstein1
  • David Erickson1,2
  • Saurabh Mehta1
  1. 1Cornell Joan Klein Jacobs Center for Precision Nutrition and Health Ithaca NY USA
  2. 2Sibley School of Mechanical and Aerospace Engineering, Cornell University Ithaca NY USA
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Abstract

Herpes simplex virus type 2 (HSV-2) is a widely prevalent human pathogen responsible for the majority of genital herpes infections. Type-specific serological screening is a valuable approach for identifying asymptomatic individuals who may unknowingly transmit HSV to sexual partners and neonates, and can inform prevention, treatment, and vaccination strategies aimed at reducing transmission. Here, we describe the development and preliminary evaluation of a Europium nanoparticle-based lateral flow assay (EuNP-LFA) for rapid detection of HSV-2-specific immunoglobulin G (IgG) antibodies in human serum within 10 minutes. Preliminary performance evaluation using a commercially available anti-herpes mixed-titer performance panel demonstrated 90% sensitivity and 90.9% specificity relative to the FDA-cleared HerpeSelect™ HSV-2 ELISA reference classification. Receiver operating characteristic analysis yielded an area under the curve of 0.918 (95% CI: 0.688–0.983), indicating good discriminatory performance in this preliminary dataset. These findings support the feasibility of the EuNP-LFA as a proof-of-concept platform for rapid HSV-2 serological testing and support further evaluation in larger, clinically representative specimen populations to establish its diagnostic performance and potential utility for point-of-care applications.

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Cite

BibTeXRIS
BibTeX
@article{srinivasan2026europium,
  title = {{Europium nanoparticle-based lateral flow assay for rapid detection of herpes simplex virus 2-specific antibodies in serum: a preliminary evaluation}},
  author = {Balaji Srinivasan and Julia L. Finkelstein and David Erickson and Saurabh Mehta},
  journal = {RSC Advances},
  year = {2026},
  month = oct,
  doi = {10.1039/d6ra04688f},
  url = {https://doi.org/10.1039/d6ra04688f},
  note = {CC BY-NC 3.0},
}
RIS
TY  - JOUR
TI  - Europium nanoparticle-based lateral flow assay for rapid detection of herpes simplex virus 2-specific antibodies in serum: a preliminary evaluation
AU  - Balaji Srinivasan
AU  - Julia L. Finkelstein
AU  - David Erickson
AU  - Saurabh Mehta
PY  - 2026
DA  - 2026/10/09
JO  - RSC Advances
DO  - 10.1039/d6ra04688f
UR  - https://doi.org/10.1039/d6ra04688f
AB  - Herpes simplex virus type 2 (HSV-2) is a widely prevalent human pathogen responsible for the majority of genital herpes infections. Type-specific serological screening is a valuable approach for identifying asymptomatic individuals who may unknowingly transmit HSV to sexual partners and neonates, and can inform prevention, treatment, and vaccination strategies aimed at reducing transmission. Here, we describe the development and preliminary evaluation of a Europium nanoparticle-based lateral flow assay (EuNP-LFA) for rapid detection of HSV-2-specific immunoglobulin G (IgG) antibodies in human serum within 10 minutes. Preliminary performance evaluation using a commercially available anti-herpes mixed-titer performance panel demonstrated 90% sensitivity and 90.9% specificity relative to the FDA-cleared HerpeSelect™ HSV-2 ELISA reference classification. Receiver operating characteristic analysis yielded an area under the curve of 0.918 (95% CI: 0.688–0.983), indicating good discriminatory performance in this preliminary dataset. These findings support the feasibility of the EuNP-LFA as a proof-of-concept platform for rapid HSV-2 serological testing and support further evaluation in larger, clinically representative specimen populations to establish its diagnostic performance and potential utility for point-of-care applications.
SN  - 2046-2069
ER  -