Telomere-to-telomere genome assembly of Coprinellus radians
4 affiliations
- 1School of Pharmacy, Anhui University of Chinese Medicine; Hefei 230012 ,
- 2Scientific Research - Healthcare Activity Direction Department, Traditional Medicine Hospital of Ho Chi Minh City , Xuan Hoa Ward 72421 ,
- 3Faculty of Pharmacy, Mahidol University , Bangkok 10400 ,
- 4School of Life Sciences, Anhui University of Chinese Medicine; Hefei 230012 ,
Abstract
Coprinellus radians has been identified as an important germination-associated fungus of Cremastra appendiculata. However, no reference genome was previously available for this species. In this study, we generated a complete telomere-to-telomere (T2T) genome assembly for C. radians by integrating PacBio HiFi, Oxford Nanopore, BGI short-read, and Hi-C sequencing data. Hi-C interaction mapping anchored 99.36% of the assembled sequences onto 13 pseudochromosomes. Following contaminant removal, gap closure using TGS-GapCloser, and telomere extension, the final assembly consists of 13 gap-free, single-contig chromosomes with telomeric repeats (CCCTAA/TTAGGG) at both ends, yielding a total of 26 telomeres. The resulting high-quality genome is 41.09 Mb (41,092,644 bp) in size, with a contig N50 of 3.36 Mb and a BUSCO completeness score of 96.4%. This gap-free, T2T genome provides a valuable resource for investigating the molecular mechanisms of orchid-fungus symbiosis and the ecological adaptation of wood-decaying fungi.
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BibTeX
@article{yu2026telomeretotelomere,
title = {{Telomere-to-telomere genome assembly of Coprinellus radians}},
author = {Manman Yu and Yuxue Cheng and Yuxin Shan and Hong To Quyen Duong and Somnuk Bunsupa and Xiaohui Tong and Rongchun Han},
journal = {G3: Genes, Genomes, Genetics},
year = {2026},
month = oct,
doi = {10.1093/g3journal/jkag267},
url = {https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag267/8898570},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Telomere-to-telomere genome assembly of Coprinellus radians
AU - Manman Yu
AU - Yuxue Cheng
AU - Yuxin Shan
AU - Hong To Quyen Duong
AU - Somnuk Bunsupa
AU - Xiaohui Tong
AU - Rongchun Han
PY - 2026
DA - 2026/10/09
JO - G3: Genes, Genomes, Genetics
DO - 10.1093/g3journal/jkag267
UR - https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag267/8898570
AB - Coprinellus radians has been identified as an important germination-associated fungus of Cremastra appendiculata. However, no reference genome was previously available for this species. In this study, we generated a complete telomere-to-telomere (T2T) genome assembly for C. radians by integrating PacBio HiFi, Oxford Nanopore, BGI short-read, and Hi-C sequencing data. Hi-C interaction mapping anchored 99.36% of the assembled sequences onto 13 pseudochromosomes. Following contaminant removal, gap closure using TGS-GapCloser, and telomere extension, the final assembly consists of 13 gap-free, single-contig chromosomes with telomeric repeats (CCCTAA/TTAGGG) at both ends, yielding a total of 26 telomeres. The resulting high-quality genome is 41.09 Mb (41,092,644 bp) in size, with a contig N50 of 3.36 Mb and a BUSCO completeness score of 96.4%. This gap-free, T2T genome provides a valuable resource for investigating the molecular mechanisms of orchid-fungus symbiosis and the ecological adaptation of wood-decaying fungi.
SN - 2160-1836
ER -