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Peer-reviewedCC BY 4.0Biomolecules

Expression Analysis of microRNAs in Peripheral Blood Mononuclear Cells in Type 2 Diabetes

  • Yanina Timasheva1,2
  • Olga Kochetova1,3
  • Vitaly A. Markelov1,4
  • Ksenia V. Danilko5
  • Tatyana M. Kochetova3
  • Diana Avzaletdinova6
  • Gulnaz F. Korytina1,3
  • Zhanna Balkhiyarova6,7
  • Arie Nouwen8
8 affiliations
  1. 1Institute of Biochemistry and Genetics, Ufa Federal Research Centre, Russian Academy of Sciences, 450054 Ufa, Russia
  2. 2Department of Medical Genetics and Fundamental Medicine, Faculty of General Medicine, Bashkir State Medical University, 450008 Ufa, Russia
  3. 3Department of Biology, Faculty of Stomatology, Bashkir State Medical University, 450008 Ufa, Russia
  4. 4Laboratory of Microfluidic Technology, Institute of Fundamental Medicine, Bashkir State Medical University, 450008 Ufa, Russia
  5. 5Laboratory of Cell Cultures, Institute of Fundamental Medicine, Bashkir State Medical University, 450008 Ufa, Russia
  6. 6Department of Endocrinology, Faculty of General Medicine, Bashkir State Medical University, 450008 Ufa, Russia
  7. 7Section of Statistical Multi-Omics, Department of Clinical & Experimental Medicine, School of Biosciences & Medicine, University of Surrey, Guildford GU2 7XH, UK
  8. 8Department of Psychology, Middlesex University, London NW4 4BT, UK
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Abstract

MicroRNAs (miRNAs) are involved in the regulation of glucose metabolism and inflammatory processes associated with type 2 diabetes (T2D) and have potential as biomarkers and therapeutic targets. We analysed the expression of miR-155-5p, miR-141-3p, miR-15b-5p, miR-18a-5p, and miR-379-5p in peripheral blood mononuclear cells (PBMCs) from 79 patients with T2D and 88 non-diabetic controls. Differential expression analysis revealed significant downregulation of miR-15b-5p, miR-155-5p, and miR-18a-5p, and significant upregulation of miR-379-5p in T2D patients. Among the individual miRNAs, miR-15b-5p showed the highest ability to distinguish between T2D and controls (AUC = 0.82, 95% CI 0.76–0.89, p = 0.001). A model combining the four differentially expressed miRNAs (miR-155-5p, miR-15b-5p, miR-18a-5p, and miR-379-5p) with age, sex, and BMI had an AUC of 0.98, compared with 0.93 for age, sex, and BMI alone (p = 0.004, paired DeLong test); the optimism-corrected AUC was 0.97, although the bootstrap procedure did not account for optimism arising from miRNA selection. Functional enrichment analysis identified MAPK, Hippo, FoxO, insulin, AMPK and TGF-β signalling as consistently enriched across analyses based on experimentally supported and predicted miRNA targets.

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Cite

BibTeXRIS
BibTeX
@article{timasheva2026expression,
  title = {{Expression Analysis of microRNAs in Peripheral Blood Mononuclear Cells in Type 2 Diabetes}},
  author = {Yanina Timasheva and Olga Kochetova and Vitaly A. Markelov and Ksenia V. Danilko and Tatyana M. Kochetova and Diana Avzaletdinova and Gulnaz F. Korytina and Zhanna Balkhiyarova and Arie Nouwen},
  journal = {Biomolecules},
  year = {2026},
  month = oct,
  doi = {10.3390/biom16101503},
  url = {https://www.mdpi.com/2218-273X/16/10/1503},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Expression Analysis of microRNAs in Peripheral Blood Mononuclear Cells in Type 2 Diabetes
AU  - Yanina Timasheva
AU  - Olga Kochetova
AU  - Vitaly A. Markelov
AU  - Ksenia V. Danilko
AU  - Tatyana M. Kochetova
AU  - Diana Avzaletdinova
AU  - Gulnaz F. Korytina
AU  - Zhanna Balkhiyarova
AU  - Arie Nouwen
PY  - 2026
DA  - 2026/10/01
JO  - Biomolecules
DO  - 10.3390/biom16101503
UR  - https://www.mdpi.com/2218-273X/16/10/1503
AB  - MicroRNAs (miRNAs) are involved in the regulation of glucose metabolism and inflammatory processes associated with type 2 diabetes (T2D) and have potential as biomarkers and therapeutic targets. We analysed the expression of miR-155-5p, miR-141-3p, miR-15b-5p, miR-18a-5p, and miR-379-5p in peripheral blood mononuclear cells (PBMCs) from 79 patients with T2D and 88 non-diabetic controls. Differential expression analysis revealed significant downregulation of miR-15b-5p, miR-155-5p, and miR-18a-5p, and significant upregulation of miR-379-5p in T2D patients. Among the individual miRNAs, miR-15b-5p showed the highest ability to distinguish between T2D and controls (AUC = 0.82, 95% CI 0.76–0.89, p = 0.001). A model combining the four differentially expressed miRNAs (miR-155-5p, miR-15b-5p, miR-18a-5p, and miR-379-5p) with age, sex, and BMI had an AUC of 0.98, compared with 0.93 for age, sex, and BMI alone (p = 0.004, paired DeLong test); the optimism-corrected AUC was 0.97, although the bootstrap procedure did not account for optimism arising from miRNA selection. Functional enrichment analysis identified MAPK, Hippo, FoxO, insulin, AMPK and TGF-β signalling as consistently enriched across analyses based on experimentally supported and predicted miRNA targets.
SN  - 2218-273X
ER  -