Affinity improvement of specific scFvs interacting with the Neisseria meningitidis factor H-binding protein: a molecular dynamics simulation approach and experimental study.
5 affiliations
- 1Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran.
- 2Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
- 3Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran. [email protected].
- 4Ludwig Boltzmann Institute of Osteology, Vienna, Austria.
- 5Cellular & Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract
Background
In silico approaches are becoming increasingly important for enhancing the affinity of antibodies and antibody fragments, including single-chain variable fragments (scFvs). In this study, we present an in silico affinity-maturation workflow designed to improve the affinity of an scFv directed against Neisseria meningitidis factor H-binding protein (fHbp) by integrating in silico maturation, molecular dynamics (MD) simulations, and experimental validation. Approximately 300 single-point substitutions were designed at scFv residues predicted to interact with fHbp, and the resulting V3 (R99D) scFv-fHbp complexes were analyzed through all-atom MD simulations. Guided by bioinformatic analyses, the top V3 (R99D) variant was cloned and expressed, and its binding activity was validated by ELISA.Results
MD analyses identified five variants, Y35H, L31R, R99D, R99H, and A100K, that exhibited markedly enhanced complex stability. In all variants, the scFv secondary structure remained intact, whereas fHbp showed shrinkage relative to the scFv in the V3 (R99D)-fHbp complexes during the simulations. Hydrogen-bonding patterns and inter-residue distances further supported the improved affinity of the variants relative to the native form. The R99D variant showed the highest binding affinity, with an experimentally measured value of 67.93 × 10⁹ M⁻¹.Conclusion
Collectively, these findings underscore affinity maturation as a powerful strategy for engineering high-affinity antibody fragments.
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BibTeX
@article{ghazanfari2026affinity,
title = {{Affinity improvement of specific scFvs interacting with the Neisseria meningitidis factor H-binding protein: a molecular dynamics simulation approach and experimental study.}},
author = {Bijan Ghazanfari and Zahra Ebrahimi and Fatemeh Yarian and Arezu Lari and Ali Ghanbariasad and Bahram Kazemi},
journal = {BMC biotechnology},
year = {2026},
month = jul,
doi = {10.1186/s12896-026-01204-3},
url = {https://europepmc.org/article/MED/42902304},
note = {CC BY},
}
RIS
TY - JOUR
TI - Affinity improvement of specific scFvs interacting with the Neisseria meningitidis factor H-binding protein: a molecular dynamics simulation approach and experimental study.
AU - Bijan Ghazanfari
AU - Zahra Ebrahimi
AU - Fatemeh Yarian
AU - Arezu Lari
AU - Ali Ghanbariasad
AU - Bahram Kazemi
PY - 2026
DA - 2026/07/01
JO - BMC biotechnology
DO - 10.1186/s12896-026-01204-3
UR - https://europepmc.org/article/MED/42902304
AB - Background In silico approaches are becoming increasingly important for enhancing the affinity of antibodies and antibody fragments, including single-chain variable fragments (scFvs). In this study, we present an in silico affinity-maturation workflow designed to improve the affinity of an scFv directed against Neisseria meningitidis factor H-binding protein (fHbp) by integrating in silico maturation, molecular dynamics (MD) simulations, and experimental validation. Approximately 300 single-point substitutions were designed at scFv residues predicted to interact with fHbp, and the resulting V3 (R99D) scFv-fHbp complexes were analyzed through all-atom MD simulations. Guided by bioinformatic analyses, the top V3 (R99D) variant was cloned and expressed, and its binding activity was validated by ELISA.Results MD analyses identified five variants, Y35H, L31R, R99D, R99H, and A100K, that exhibited markedly enhanced complex stability. In all variants, the scFv secondary structure remained intact, whereas fHbp showed shrinkage relative to the scFv in the V3 (R99D)-fHbp complexes during the simulations. Hydrogen-bonding patterns and inter-residue distances further supported the improved affinity of the variants relative to the native form. The R99D variant showed the highest binding affinity, with an experimentally measured value of 67.93 × 10⁹ M⁻¹.Conclusion Collectively, these findings underscore affinity maturation as a powerful strategy for engineering high-affinity antibody fragments.
SN - 1472-6750
ER -