Phenotypic Characteristics of B-Cell Subsets in Children with Allergic Diseases.
- 1Faculty of Medicine, University of Warsaw, 02-089 Warsaw, Poland.
- 2Department of Pediatrics, Nephrology and Allergology, Military Institute of Medicine-National Research Institute, 01-141 Warsaw, Poland.
- 3Laboratory of Molecular Oncology and Innovative Therapies, Military Institute of Medicine National Research Institute, 04-141 Warsaw, Poland.
Abstract
B-cell subpopulations are increasingly recognized as important modulators of immune responses, yet their role in pediatric allergic diseases remains incompletely understood. This study aimed to characterize the phenotype and frequency of peripheral B-cell subsets in children with allergic diseases and to assess their relationship with clinical and laboratory parameters. The study included 59 children with asthma, atopic dermatitis, or allergic rhinitis and 19 healthy controls. Multicolor flow cytometry was used to analyze B-cell subpopulations, including CD19⁺CD24hiCD38hi, CD19⁺CD25⁺, and IgD⁻CD27⁺ cells. The children with allergic diseases exhibited significantly reduced percentages of CD19⁺CD25⁺ B cells and switched-memory B cells compared with the controls. Negative correlations were observed between selected B-cell subsets and eosinophil counts, suggesting an association with type 2 inflammation. In asthma, higher proportions of transitional B cells were associated with better disease control, whereas no significant relationship was found between B-cell subsets and atopic dermatitis severity. In allergic rhinitis, the frequency of CD19⁺CD25⁺FoxP3⁺ B cells differed according to disease control, with lower frequencies observed in children with uncontrolled compared with partially controlled disease. These findings indicate that changes in B-cell populations may be involved in the pathogenesis of allergic diseases in children and their clinical expression.
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BibTeX
@article{lipinskaopalka2026phenotypic,
title = {{Phenotypic Characteristics of B-Cell Subsets in Children with Allergic Diseases.}},
author = {Agnieszka Lipińska-Opałka and Agata Tomaszewska and Monika Leśnik and Robert Zdanowski and Michalina Leszczyńska-Pilich and Bolesław Kalicki},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198600},
url = {https://europepmc.org/article/MED/42883848},
note = {CC BY},
}
RIS
TY - JOUR
TI - Phenotypic Characteristics of B-Cell Subsets in Children with Allergic Diseases.
AU - Agnieszka Lipińska-Opałka
AU - Agata Tomaszewska
AU - Monika Leśnik
AU - Robert Zdanowski
AU - Michalina Leszczyńska-Pilich
AU - Bolesław Kalicki
PY - 2026
DA - 2026/09/25
JO - International journal of molecular sciences
DO - 10.3390/ijms27198600
UR - https://europepmc.org/article/MED/42883848
AB - B-cell subpopulations are increasingly recognized as important modulators of immune responses, yet their role in pediatric allergic diseases remains incompletely understood. This study aimed to characterize the phenotype and frequency of peripheral B-cell subsets in children with allergic diseases and to assess their relationship with clinical and laboratory parameters. The study included 59 children with asthma, atopic dermatitis, or allergic rhinitis and 19 healthy controls. Multicolor flow cytometry was used to analyze B-cell subpopulations, including CD19⁺CD24hiCD38hi, CD19⁺CD25⁺, and IgD⁻CD27⁺ cells. The children with allergic diseases exhibited significantly reduced percentages of CD19⁺CD25⁺ B cells and switched-memory B cells compared with the controls. Negative correlations were observed between selected B-cell subsets and eosinophil counts, suggesting an association with type 2 inflammation. In asthma, higher proportions of transitional B cells were associated with better disease control, whereas no significant relationship was found between B-cell subsets and atopic dermatitis severity. In allergic rhinitis, the frequency of CD19⁺CD25⁺FoxP3⁺ B cells differed according to disease control, with lower frequencies observed in children with uncontrolled compared with partially controlled disease. These findings indicate that changes in B-cell populations may be involved in the pathogenesis of allergic diseases in children and their clinical expression.
SN - 1422-0067
ER -