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Peer-reviewedCC BYJournal of veterinary science

In silico characterization of ferritin 2 conserved motifs and B-cell epitopes in Hyalomma anatolicum.

  • Xueqing Zhao1,2
  • Zeyun Cui1,2
  • Na Zhou1,2
  • Yuqian Deng1,2
  • Licui Wen1,2
  • Wenying Dang1,2
  • Caishan Li3
  • Bayinchahan Gailike1,4
4 affiliations
  1. 1College of Animal Medicine, Xinjiang Agricultural University, Urumqi 830052, China.
  2. 2Xinjiang Key Laboratory of Herbivore New Drug Research and Creation, Urumqi 830052, China.
  3. 3College of Animal Science and Technology, Xinjiang Agricultural Vocational and Technical University, Changji 831100, China. [email protected].
  4. 4Xinjiang Key Laboratory of Herbivore New Drug Research and Creation, Urumqi 830052, China. [email protected].
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Abstract

Importance

Ferritin 2 (Fer2) is a well-established candidate antigen for anti-tick vaccines, but few studies have investigated the potential of Hyalomma anatolicum Fer2 as an antigenic peptide.Objective

To characterize the H. anatolicum fer 2 coding sequence, conserved motifs, predicted structure, and linear B-cell epitopes.Methods

The complete coding sequence region of the H. anatolicum Fer2 gene was amplified by polymerase chain reaction. The biological properties and effective antigenic peptide of H. anatolicum Fer2 protein were analyzed.Results

The Fer2 sequence of H. anatolicum collected showed the highest similarity to that of H. anatolicum from Turpan, Xinjiang (accession No. ALJ92580.1), and the phylogenetic analysis accurately classified Fer2 among the different tick species. The biological property results showed that H. anatolicum Fer2 is a non-transmembrane protein with a signal peptide and a typical Fer protein structure. Motif analysis showed that different tick 5 motifs were in random combinations, but motifs 1, 2, and 4 were contained in all tick Fer2 proteins. Correspondence existed between motifs and antigens, with motif 1 containing the highest-scoring antigenic epitope.Conclusions and relevance

H. anatolicum fer 2 motif 1 contains a conserved, high-scoring predicted linear B-cell epitope and warrants experimental immunogenicity and protection studies before consideration as an anti-tick vaccine peptide.

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Cite

BibTeXRIS
BibTeX
@article{zhao2026silico,
  title = {{In silico characterization of ferritin 2 conserved motifs and B-cell epitopes in Hyalomma anatolicum.}},
  author = {Xueqing Zhao and Zeyun Cui and Na Zhou and Yuqian Deng and Licui Wen and Wenying Dang and Caishan Li and Bayinchahan Gailike},
  journal = {Journal of veterinary science},
  year = {2026},
  month = sep,
  doi = {10.4142/jvs.25152},
  url = {https://europepmc.org/article/MED/42847793},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - In silico characterization of ferritin 2 conserved motifs and B-cell epitopes in Hyalomma anatolicum.
AU  - Xueqing Zhao
AU  - Zeyun Cui
AU  - Na Zhou
AU  - Yuqian Deng
AU  - Licui Wen
AU  - Wenying Dang
AU  - Caishan Li
AU  - Bayinchahan Gailike
PY  - 2026
DA  - 2026/09/01
JO  - Journal of veterinary science
DO  - 10.4142/jvs.25152
UR  - https://europepmc.org/article/MED/42847793
AB  - Importance  Ferritin 2 (Fer2) is a well-established candidate antigen for anti-tick vaccines, but few studies have investigated the potential of Hyalomma anatolicum Fer2 as an antigenic peptide.Objective  To characterize the H. anatolicum fer 2 coding sequence, conserved motifs, predicted structure, and linear B-cell epitopes.Methods  The complete coding sequence region of the H. anatolicum Fer2 gene was amplified by polymerase chain reaction. The biological properties and effective antigenic peptide of H. anatolicum Fer2 protein were analyzed.Results  The Fer2 sequence of H. anatolicum collected showed the highest similarity to that of H. anatolicum from Turpan, Xinjiang (accession No. ALJ92580.1), and the phylogenetic analysis accurately classified Fer2 among the different tick species. The biological property results showed that H. anatolicum Fer2 is a non-transmembrane protein with a signal peptide and a typical Fer protein structure. Motif analysis showed that different tick 5 motifs were in random combinations, but motifs 1, 2, and 4 were contained in all tick Fer2 proteins. Correspondence existed between motifs and antigens, with motif 1 containing the highest-scoring antigenic epitope.Conclusions and relevance  H. anatolicum fer 2 motif 1 contains a conserved, high-scoring predicted linear B-cell epitope and warrants experimental immunogenicity and protection studies before consideration as an anti-tick vaccine peptide.
SN  - 1229-845X
SN  - 1976-555X
ER  -