A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
Abstract
The effectiveness of nanoparticles (NP) in nanomedicine depends on their ability to extravasate from vasculature towards the target tissue. This is determined by their permeability across the endothelial barrier. Unfortunately, a quantitative study of the diffusion permeability coefficients (Pd) of NPs is difficult within vivomodels. Here, we utilize a relevant model of vascular-tissue interface with tunable endothelial permeabilityin vitrobased on microfluidics. Human umbilical vein endothelial cells (HUVECs) grown in microfluidic devices were treated with Angiopoietin 1 and cyclic adenosine monophosphate (cAMP) to vary the Pdof the HUVECs monolayer towards fluorescent polystyrene NPs (pNPs) of different sizes, which was determined from image analysis of their fluorescence intensity when diffusing across the monolayer. Using 70 kDa dextran as a probe, untreated HUVECs yielded a Pdthat approximated tumor vasculature while HUVECs treated with 25 μg/mL cAMP had Pdthat approximated healthy vasculaturein vivo. As the size of pNPs increased, its Pddecreased in tumor vasculature, but remained largely unchanged in healthy vasculature, demonstrating a trend similar to tumor selectivity for smaller NPs. This microfluidic model of vascular-tissue interface can be used in any laboratory to perform quantitative assessment of the tumor selectivity of nanomedicine-based systems.
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BibTeX
@article{ho2026facile,
title = {{A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications}},
author = {Yan Teck Ho and Giulia Adriani and Sebastian Beyer and N. Phan-Thien and Roger D. Kamm and James Chen Yong Kah},
journal = {Scientific Reports},
year = {2026},
month = oct,
doi = {10.1038/s41598-017-00750-3},
url = {https://hal.science/hal-05776453v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - A Facile Method to Probe the Vascular Permeability of Nanoparticles in Nanomedicine Applications
AU - Yan Teck Ho
AU - Giulia Adriani
AU - Sebastian Beyer
AU - N. Phan-Thien
AU - Roger D. Kamm
AU - James Chen Yong Kah
PY - 2026
DA - 2026/10/03
JO - Scientific Reports
DO - 10.1038/s41598-017-00750-3
UR - https://hal.science/hal-05776453v1
AB - The effectiveness of nanoparticles (NP) in nanomedicine depends on their ability to extravasate from vasculature towards the target tissue. This is determined by their permeability across the endothelial barrier. Unfortunately, a quantitative study of the diffusion permeability coefficients (Pd) of NPs is difficult within vivomodels. Here, we utilize a relevant model of vascular-tissue interface with tunable endothelial permeabilityin vitrobased on microfluidics. Human umbilical vein endothelial cells (HUVECs) grown in microfluidic devices were treated with Angiopoietin 1 and cyclic adenosine monophosphate (cAMP) to vary the Pdof the HUVECs monolayer towards fluorescent polystyrene NPs (pNPs) of different sizes, which was determined from image analysis of their fluorescence intensity when diffusing across the monolayer. Using 70 kDa dextran as a probe, untreated HUVECs yielded a Pdthat approximated tumor vasculature while HUVECs treated with 25 μg/mL cAMP had Pdthat approximated healthy vasculaturein vivo. As the size of pNPs increased, its Pddecreased in tumor vasculature, but remained largely unchanged in healthy vasculature, demonstrating a trend similar to tumor selectivity for smaller NPs. This microfluidic model of vascular-tissue interface can be used in any laboratory to perform quantitative assessment of the tumor selectivity of nanomedicine-based systems.
SN - 2045-2322
ER -