Niallia Circulans mediated Bio-fabrication of ZnO nanoparticles: Synthesis, characterization, and multifunctional applications
7 affiliations
- 1Department of Life Sciences, Parul Institute of Applied Sciences, Parul University, Vadodara, Gujarat, India
- 2Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India
- 3Department of Biomedical Engineering, North Eastern Hill University, Shillong, Meghalaya, India
- 4Department of Biosciences, School of Liberal Arts and Sciences, Mody University of Sciences and Technology, Lakshmangarh, Sikar (Rajasthan), India
- 5Independent Researcher, Ames, Iowa, United States of America
- 6Gujarat State Biotechnology Mission (GSBTM), Udyog Bhavan, Gandhinagar, Gujarat, India
- 7Gujarat Biotechnology Research Centre (GBRC), M. S. Building, Gandhinagar, Gujarat, India
Abstract
The current study addresses increasing global problems (agricultural sustainability, environmental contamination, and antimicrobial resistance) by means of sustainable and multifunctional biosynthesized Zinc oxide nanoparticles (ZnO NPs). The study utilized a cell-free supernatant of a novel endophytic bacterial strain (Niallia Circulans) to synthesize ZnO NPs. Biosynthesized NPs were evaluated by using various techniques of characterization. Surface plasmon resonance (SPR) was revealed by UV-Visible spectral peak for ZnO NPs at 355 nm. A X-ray diffraction (XRD) analysis confirmed a crystallite size of 90.64 nm, while scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) demonstrated the predominance of hexagonal-structured zinc, carbon, and oxygen elements. The functional groups in Fourier transform infrared spectroscopy (FTIR) confirm the presence of biomolecules. The evaluated multifunctional application of biosynthesized ZnO NPs exhibits an enhanced early seedling growth rate and increased seed germination (100% in 12 h) on cowpea (Vigna unguiculata L.) compared to the control groups. Also shows excellent antibacterial activity against test bacterial strains. Additionally, a high removal efficiency of 97.85% was achieved within 110 min for 10 ppm methylene blue dye solution at pH 9. This study highlights the endophytic bacteria-mediated successful synthesis of ZnO NPs, with potential multifunctional applications in agriculture, antimicrobial treatments, and environmental remediation.
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BibTeX
@article{prasad2026niallia,
title = {{Niallia Circulans mediated Bio-fabrication of ZnO nanoparticles: Synthesis, characterization, and multifunctional applications}},
author = {Kiran Prasad and Bhakti Patel and Gautam Priyadarshi and Dinesh Bhatia and Rakesh Kumar Verma and Esha Rami and Dipak Kumar Sahoo and Snehal Bagatharia and Ashish Patel},
journal = {PLOS One},
year = {2026},
month = oct,
doi = {10.1371/journal.pone.0360387},
url = {https://doi.org/10.1371/journal.pone.0360387},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Niallia Circulans mediated Bio-fabrication of ZnO nanoparticles: Synthesis, characterization, and multifunctional applications
AU - Kiran Prasad
AU - Bhakti Patel
AU - Gautam Priyadarshi
AU - Dinesh Bhatia
AU - Rakesh Kumar Verma
AU - Esha Rami
AU - Dipak Kumar Sahoo
AU - Snehal Bagatharia
AU - Ashish Patel
PY - 2026
DA - 2026/10/12
JO - PLOS One
DO - 10.1371/journal.pone.0360387
UR - https://doi.org/10.1371/journal.pone.0360387
AB - The current study addresses increasing global problems (agricultural sustainability, environmental contamination, and antimicrobial resistance) by means of sustainable and multifunctional biosynthesized Zinc oxide nanoparticles (ZnO NPs). The study utilized a cell-free supernatant of a novel endophytic bacterial strain (Niallia Circulans) to synthesize ZnO NPs. Biosynthesized NPs were evaluated by using various techniques of characterization. Surface plasmon resonance (SPR) was revealed by UV-Visible spectral peak for ZnO NPs at 355 nm. A X-ray diffraction (XRD) analysis confirmed a crystallite size of 90.64 nm, while scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) demonstrated the predominance of hexagonal-structured zinc, carbon, and oxygen elements. The functional groups in Fourier transform infrared spectroscopy (FTIR) confirm the presence of biomolecules. The evaluated multifunctional application of biosynthesized ZnO NPs exhibits an enhanced early seedling growth rate and increased seed germination (100% in 12 h) on cowpea (Vigna unguiculata L.) compared to the control groups. Also shows excellent antibacterial activity against test bacterial strains. Additionally, a high removal efficiency of 97.85% was achieved within 110 min for 10 ppm methylene blue dye solution at pH 9. This study highlights the endophytic bacteria-mediated successful synthesis of ZnO NPs, with potential multifunctional applications in agriculture, antimicrobial treatments, and environmental remediation.
SN - 1932-6203
ER -