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Peer-reviewedCC BY 4.0Biomolecules

Sequence-Engineered Recombinant Human Collagen as a Biomacromolecular Platform for Guided Bone Regeneration Membranes

  • Jianing Hou1
  • Zhuoyue Chen1
  • Meihua Wang1
  • Zhen Tian2
  • Fulin Chen1
  • Yuan Li2
  • Xue Feng1
  1. 1School of Medicine, Northwest University, Xi’an 710069, China
  2. 2Medical Research and Development Center, Shaanxi Huikang Bio-Tech Co., Ltd., Xi’an 710054, China
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Abstract

Effective repair of bone defects remains a major clinical challenge. Guided bone regeneration (GBR) membranes promote bone regeneration by preventing soft-tissue invasion; however, synthetic membranes often exhibit limited bioactivity, whereas animal-derived collagen membranes face concerns related to source safety, immunogenicity, and batch variability. Here, we developed a sequence-engineered recombinant human collagen (rhCol)-based biomaterial platform and fabricated a degradable bilayer membrane (rhColM) through dehydrothermal treatment free of exogenous chemical crosslinkers. rhColM exhibited a tensile strength of 5.44 MPa, a porosity of 76.26%, and an asymmetric architecture consisting of a dense barrier layer and a porous bone-facing layer. In rat subcutaneous implantation, rhColM showed favorable biocompatibility without obvious inflammatory cell infiltration or connective tissue penetration at 1 and 3 weeks. In a rabbit lateral femoral condyle defect model, rhColM increased bone volume fraction to 42.54%, 90.70% and 99.41% at 4, 8 and 12 weeks, respectively, compared with 36.19%, 76.36% and 90.15% in controls. Histological analysis revealed progressive bone formation along the rhColM interface and substantial defect repair with lamellar bone-like tissue by 8 weeks, while rhColM was no longer visibly detected. These findings highlight sequence-engineered rhCol as a promising and source-defined biomacromolecular platform for developing recombinant collagen-based GBR membranes.

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Cite

BibTeXRIS
BibTeX
@article{hou2026sequenceengineered,
  title = {{Sequence-Engineered Recombinant Human Collagen as a Biomacromolecular Platform for Guided Bone Regeneration Membranes}},
  author = {Jianing Hou and Zhuoyue Chen and Meihua Wang and Zhen Tian and Fulin Chen and Yuan Li and Xue Feng},
  journal = {Biomolecules},
  year = {2026},
  month = oct,
  doi = {10.3390/biom16111561},
  url = {https://www.mdpi.com/2218-273X/16/11/1561},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Sequence-Engineered Recombinant Human Collagen as a Biomacromolecular Platform for Guided Bone Regeneration Membranes
AU  - Jianing Hou
AU  - Zhuoyue Chen
AU  - Meihua Wang
AU  - Zhen Tian
AU  - Fulin Chen
AU  - Yuan Li
AU  - Xue Feng
PY  - 2026
DA  - 2026/10/26
JO  - Biomolecules
DO  - 10.3390/biom16111561
UR  - https://www.mdpi.com/2218-273X/16/11/1561
AB  - Effective repair of bone defects remains a major clinical challenge. Guided bone regeneration (GBR) membranes promote bone regeneration by preventing soft-tissue invasion; however, synthetic membranes often exhibit limited bioactivity, whereas animal-derived collagen membranes face concerns related to source safety, immunogenicity, and batch variability. Here, we developed a sequence-engineered recombinant human collagen (rhCol)-based biomaterial platform and fabricated a degradable bilayer membrane (rhColM) through dehydrothermal treatment free of exogenous chemical crosslinkers. rhColM exhibited a tensile strength of 5.44 MPa, a porosity of 76.26%, and an asymmetric architecture consisting of a dense barrier layer and a porous bone-facing layer. In rat subcutaneous implantation, rhColM showed favorable biocompatibility without obvious inflammatory cell infiltration or connective tissue penetration at 1 and 3 weeks. In a rabbit lateral femoral condyle defect model, rhColM increased bone volume fraction to 42.54%, 90.70% and 99.41% at 4, 8 and 12 weeks, respectively, compared with 36.19%, 76.36% and 90.15% in controls. Histological analysis revealed progressive bone formation along the rhColM interface and substantial defect repair with lamellar bone-like tissue by 8 weeks, while rhColM was no longer visibly detected. These findings highlight sequence-engineered rhCol as a promising and source-defined biomacromolecular platform for developing recombinant collagen-based GBR membranes.
SN  - 2218-273X
ER  -