Skip to content
DraconiticResearch
Search Sources API
197,768 studies31 sourcesUpdated 53 min ago
Peer-reviewedCC BY-NC-ND 4.0Cancer Genomics & Proteomics

Multi-omics Profiling of Post-translational Modifications in RNA-binding Proteins in Colorectal Cancer

  • YANBIN DONG1,2
  • WENXUAN RUAN3
  • DONGXUE QI1
  • DONGE TANG4
  • YONG DAI5
  • WEI ZHANG6
  • YI LIU1
6 affiliations
  1. 1Department of Pathology, Lianyungang Clinical College of Nanjing Medical University, The First People’s Hospital of Lianyungang, Lianyungang, P.R. China
  2. 2Lianyungang Medical Education and Innovation Research Center, Nanjing Medical University, Lianyungang, P.R. China
  3. 3Zhongshan School of Medicine, Sun Yat-sen University, Shenzhen, P.R. China
  4. 4Clinical Medical Research Center, The First Affiliated Hospital (Shenzhen People’s Hospital), Southern University of Science and Technology, Shenzhen, P.R. China
  5. 5The First Affiliated Hospital, School of Medicine, Anhui University of Science and Technology, Huainan, P.R. China
  6. 6Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, P.R. China
Read full textDOICite

Abstract

Background/Aim: Post-translational modifications (PTMs) play a pivotal role in regulating RNA-binding proteins (RBPs), which are crucial in the progression of colorectal cancer (CRC). This study aims to explore the role of PTMs in modulating RBPs and their implications for CRC pathogenesis.

Materials and Methods: We characterized phosphorylation, ubiquitination, and malonylation of RBPs in CRC using previously generated post-translational multi-omics datasets from paired CRC and adjacent non-tumor tissues in our previous studies. Functional enrichment analysis was conducted to identify pathways associated with RBPs carrying different PTMs in CRC. Protein-protein interaction (PPI) networks were generated using STRING, and hub genes were identified with the Cytoscape plugin CytoHubba. We also investigated the intersection of RBPs with distinct PTMs and evaluated the expression and prognostic relevance of FLNA (filamin A).

Results: Our findings revealed aberrant PTMs of RBPs in CRC, with ubiquitination sites showing the most frequent alterations, followed by phosphorylation, while malonylation exhibited the fewest changes. Functional enrichment analysis indicated that these PTMs contribute to distinct biological processes, including RNA metabolism, protein synthesis, and translation regulation. PPI network analysis showed that RBPs with different PTMs were interconnected in complex networks, identifying several potential RBPs closely related to CRC. Notably, FLNA emerged as a unique RBP modified by all three PTMs. FLNA mRNA and protein abundance were reduced in CRC, and phosphorylation of FLNA at S2319 was also decreased. Higher FLNA expression was associated with worse overall survival. FLNA-associated genes were enriched in pathways related to epithelial-mesenchymal transition (EMT) and KRAS signaling.

Conclusion: Multi-PTM profiling revealed distinct and convergent modification patterns among CRC-associated RBPs. FLNA emerged as a potential multi-PTM candidate associated with CRC-related molecular and clinical features. These findings provide a framework for further investigating the biological relevance of PTM-regulated RBPs and their potential implications for the development of molecularly targeted strategies in CRC.

Related

  1. Profiling protein post-translational modifications in plasma-derived extracellular vesicles as fingerprints for breast cancer subtypes.
    Marco Hadisurya, Hillary Andaluz Aguilar, I-Hsuan Chen +9
    Genome biologyPeer-reviewedCC BY-NC-NDFull textMolecular biology & gene regulationEurope PMCDOAJ
  2. Comprehensive multi-post-translational modifications profiling reveals age-associated remodeling in skeletal muscle
    Jian Li, Xin-Qiang Lan, Cai-Fen Guo +7
    Zoological ResearchPeer-reviewedCC BY-NC 4.0Full textMolecular biology & gene regulationPMC
  3. A Graphormer approach for predicting peptide half-life, secondary structure, solvent accessibility, and post-translational modifications
    Paula Cifuentes, Ramon Adàlia, Lisa A. Vasicek +3
    Scientific ReportsPeer-reviewedCC BY-NC-ND 4.0Full textMolecular biology & gene regulationCrossref
  4. Networked control of bone homeostasis: interfacing post-translational modifications with cellular signaling
    Yunpeng Zou, Jianning Kang, Zheng Li +6
    BMC MedicinePeer-reviewedCC BY-NC-ND 4.0Molecular biology & gene regulationCrossref
  5. Crosstalk between ubiquitination and other post-translational modifications in cardiovascular diseases.
    Yi Yan, Zhiqiang Li, Tingting Xiao
    ISciencePeer-reviewedCC BYFull textMolecular biology & gene regulationEurope PMC

Cite

BibTeXRIS
BibTeX
@article{dong2026multiomics,
  title = {{Multi-omics Profiling of Post-translational Modifications in RNA-binding Proteins in Colorectal Cancer}},
  author = {YANBIN DONG and WENXUAN RUAN and DONGXUE QI and DONGE TANG and YONG DAI and WEI ZHANG and YI LIU},
  journal = {Cancer Genomics \& Proteomics},
  year = {2026},
  month = oct,
  doi = {10.21873/cgp.20620},
  url = {https://doi.org/10.21873/cgp.20620},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - Multi-omics Profiling of Post-translational Modifications in RNA-binding Proteins in Colorectal Cancer
AU  - YANBIN DONG
AU  - WENXUAN RUAN
AU  - DONGXUE QI
AU  - DONGE TANG
AU  - YONG DAI
AU  - WEI ZHANG
AU  - YI LIU
PY  - 2026
DA  - 2026/10/31
JO  - Cancer Genomics & Proteomics
DO  - 10.21873/cgp.20620
UR  - https://doi.org/10.21873/cgp.20620
AB  - Background/Aim: Post-translational modifications (PTMs) play a pivotal role in regulating RNA-binding proteins (RBPs), which are crucial in the progression of colorectal cancer (CRC). This study aims to explore the role of PTMs in modulating RBPs and their implications for CRC pathogenesis.  Materials and Methods: We characterized phosphorylation, ubiquitination, and malonylation of RBPs in CRC using previously generated post-translational multi-omics datasets from paired CRC and adjacent non-tumor tissues in our previous studies. Functional enrichment analysis was conducted to identify pathways associated with RBPs carrying different PTMs in CRC. Protein-protein interaction (PPI) networks were generated using STRING, and hub genes were identified with the Cytoscape plugin CytoHubba. We also investigated the intersection of RBPs with distinct PTMs and evaluated the expression and prognostic relevance of FLNA (filamin A).  Results: Our findings revealed aberrant PTMs of RBPs in CRC, with ubiquitination sites showing the most frequent alterations, followed by phosphorylation, while malonylation exhibited the fewest changes. Functional enrichment analysis indicated that these PTMs contribute to distinct biological processes, including RNA metabolism, protein synthesis, and translation regulation. PPI network analysis showed that RBPs with different PTMs were interconnected in complex networks, identifying several potential RBPs closely related to CRC. Notably, FLNA emerged as a unique RBP modified by all three PTMs. FLNA mRNA and protein abundance were reduced in CRC, and phosphorylation of FLNA at S2319 was also decreased. Higher FLNA expression was associated with worse overall survival. FLNA-associated genes were enriched in pathways related to epithelial-mesenchymal transition (EMT) and KRAS signaling.  Conclusion: Multi-PTM profiling revealed distinct and convergent modification patterns among CRC-associated RBPs. FLNA emerged as a potential multi-PTM candidate associated with CRC-related molecular and clinical features. These findings provide a framework for further investigating the biological relevance of PTM-regulated RBPs and their potential implications for the development of molecularly targeted strategies in CRC.
SN  - 1109-6535
SN  - 1790-6245
ER  -