Skip to content
DraconiticResearch
Search Sources API
201,683 studies31 sourcesUpdated 31 min ago
Peer-reviewedCC BY 4.0ACS Omega

Structure–Function Relationships of a High-Molecular-Weight Exopolysaccharide from Euhalothece sp. with Bioactive and Emulsifying Properties

  • Kübra Şentürk Azak1
  • Mark Klöpfer2
  • Emel Tamahkar Irmak1
  • Gökçe Taner1
  • Wolfgang Eisenreich2
  • Mete Yilmaz1
  1. 1Bursa Technical University , , ,
  2. 2Technical University of Munich , , Lichtenbergstr. 4 , ,
Open at sourcePDFDOICite

Abstract

Cyanobacterial exopolysaccharides (EPSs) are promising sustainable biopolymers, yet halotolerant strains remain comparatively underexplored and the relationships between EPS composition and functionality are still not well-defined. In this study, an EPS produced by Euhalothece sp. AQUAMEB-3 was isolated and characterized to assess its physicochemical properties, compositional features, and multifunctional performance. The EPS was identified as a high-molecular-weight (Mw ∼ 2617 kDa), strongly anionic heteropolysaccharide with a zeta potential of −45.1 mV, composed mainly of xylose, fucose, amino sugars, and uronic acids. FTIR, 1H NMR, 1H–1H correlation spectroscopy (COSY) NMR, and GC–MS analyses indicated a structurally heterogeneous polymer, while XRD and SEM revealed a predominantly amorphous and porous morphology. The EPS also exhibited pronounced shear-thinning behavior and strong water affinity. Functionally, the EPS showed high emulsifying performance, with E24 values reaching 94.2% at 1.0% (w/v), comparable to xanthan gum under the tested conditions, and maintained emulsion stability over extended storage. It also displayed antibacterial activity against Escherichia coli (MIC: 150 μg mL–1) and Staphylococcus aureus (MIC: 300 μg mL–1), together mL–1 with concentration-dependent antioxidant and iron-chelating activities, reaching 82.6% radical scavenging and 96% metal chelation at the highest tested concentrations. Cytotoxicity assays indicated concentration- and cell-type-dependent cytocompatibility. Taken together, these results define a distinct physicochemical and functional profile for the EPS produced by Euhalothece sp. AQUAMEB-3 and expand the available characterization data for halotolerant cyanobacterial exopolysaccharides. The observed associations between its compositional and physicochemical characteristics and functional properties should be regarded as correlative rather than mechanistically established. These findings support the potential of AQUAMEB-3 EPS for further investigation in emulsion stabilization and other biobased formulation applications.

Related

  1. Relationship between the emulsifying properties of Acacia gums and the retention and diffusion of aroma compounds
    Géraldine Savary, Nicolas Hucher, Estelle Bernadi +2
    Food HydrocolloidsPeer-reviewedPublisher termsFull text26 citedAnalytical chemistryMaterials & polymersHAL
  2. The Emulsifying Properties of Rice-Based Fermented Foods: Contribution of the Insoluble Fraction with Potential Protein Involvement
    Kazuya Kobayashi, Moemi Anada, Wataru Noro +6
    Journal of Oleo SciencePeer-reviewedLicence not statedFull textGeneral chemistryJ-STAGE
  3. Structure–Property Relationships of PAM/CMC-Based Semi-IPN Hydrogels: Influence of APS/ANI Ratio on Swelling, Electrical, Thermal, and Morphological Properties
    Denis W. S. Nascimento, Marcia R. de Moura, Jose A. Malmonge +1
    ACS OmegaPeer-reviewedCC BY 4.0Full textMaterials & polymersCrossrefEurope PMCEurope PMCPMC
  4. Comparative study on the amphiphilicity, emulsifying and foaming properties of saponins extracted from Furcraea foetida
    Tiana Randriamamonjy, Jesús Ontiveros, Mbolatiana Tovo Andrianjafy +6
    Colloids and Surfaces A: Physicochemical and Engineering AspectsPeer-reviewedLicence not stated19 citedOrganic chemistryHAL
  5. Enzyme-assisted development of food-grade Pickering stabilizers from beer bagasse with enhanced emulsifying properties
    César Burgos-Díaz, Yohanna Mosi-Roa, Fernando Leal-Calderón +7
    Food and Bioprocess TechnologyPeer-reviewedCC BY-NC-ND 4.0Full textMaterials & polymersHAL

Cite

BibTeXRIS
BibTeX
@article{azak2026structurefunction,
  title = {{Structure–Function Relationships of a High-Molecular-Weight Exopolysaccharide from Euhalothece sp. with Bioactive and Emulsifying Properties}},
  author = {Kübra Şentürk Azak and Mark Klöpfer and Emel Tamahkar Irmak and Gökçe Taner and Wolfgang Eisenreich and Mete Yilmaz},
  journal = {ACS Omega},
  year = {2026},
  month = oct,
  doi = {10.1021/acsomega.6c04930},
  url = {https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c04930/5458012/Structure-Function-Relationships-of-a-High},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Structure–Function Relationships of a High-Molecular-Weight Exopolysaccharide from Euhalothece sp. with Bioactive and Emulsifying Properties
AU  - Kübra Şentürk Azak
AU  - Mark Klöpfer
AU  - Emel Tamahkar Irmak
AU  - Gökçe Taner
AU  - Wolfgang Eisenreich
AU  - Mete Yilmaz
PY  - 2026
DA  - 2026/10/26
JO  - ACS Omega
DO  - 10.1021/acsomega.6c04930
UR  - https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c04930/5458012/Structure-Function-Relationships-of-a-High
AB  - Cyanobacterial exopolysaccharides (EPSs) are promising sustainable biopolymers, yet halotolerant strains remain comparatively underexplored and the relationships between EPS composition and functionality are still not well-defined. In this study, an EPS produced by Euhalothece sp. AQUAMEB-3 was isolated and characterized to assess its physicochemical properties, compositional features, and multifunctional performance. The EPS was identified as a high-molecular-weight (Mw ∼ 2617 kDa), strongly anionic heteropolysaccharide with a zeta potential of −45.1 mV, composed mainly of xylose, fucose, amino sugars, and uronic acids. FTIR, 1H NMR, 1H–1H correlation spectroscopy (COSY) NMR, and GC–MS analyses indicated a structurally heterogeneous polymer, while XRD and SEM revealed a predominantly amorphous and porous morphology. The EPS also exhibited pronounced shear-thinning behavior and strong water affinity. Functionally, the EPS showed high emulsifying performance, with E24 values reaching 94.2% at 1.0% (w/v), comparable to xanthan gum under the tested conditions, and maintained emulsion stability over extended storage. It also displayed antibacterial activity against Escherichia coli (MIC: 150 μg mL–1) and Staphylococcus aureus (MIC: 300 μg mL–1), together mL–1 with concentration-dependent antioxidant and iron-chelating activities, reaching 82.6% radical scavenging and 96% metal chelation at the highest tested concentrations. Cytotoxicity assays indicated concentration- and cell-type-dependent cytocompatibility. Taken together, these results define a distinct physicochemical and functional profile for the EPS produced by Euhalothece sp. AQUAMEB-3 and expand the available characterization data for halotolerant cyanobacterial exopolysaccharides. The observed associations between its compositional and physicochemical characteristics and functional properties should be regarded as correlative rather than mechanistically established. These findings support the potential of AQUAMEB-3 EPS for further investigation in emulsion stabilization and other biobased formulation applications.
SN  - 2470-1343
ER  -