Gradient Ethanol Precipitation of Sanghuangporus vaninii Polysaccharides: Physicochemical Properties and Inhibition of Tumor Cell Proliferation.
- 1Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, China.
- 2Yanbian Special Industry Development Center, Yanji 133002, China.
- 3Department of Pharmacy, Jilin Medical University, Jilin 132013, China.
Abstract
Three polysaccharide fractions (SH-40, SH-60, and SH-80) were sequentially separated from Sanghuangporus vaninii fruiting bodies through graded ethanol precipitation to investigate the influence of molecular weight on their structure, physicochemical properties, and inhibition of tumor cell proliferation. The results revealed that the molecular weights of these three fractions were approximately 5.90 × 10⁵, 4.82 × 10⁵, and 4.13 × 10⁵ Da, respectively; they shared similar functional group profiles, monosaccharide compositions and glycosidic bonds. As the molecular weight decreased, the water holding capacity (WHC) declined, while the water solubility index (WSI) and oil holding capacity (OHC) increased. In tumor cell proliferation inhibition assays, all three fractions inhibited the proliferation of MCF-7, HepG2, A549, and SGC-7901 cells in a concentration-dependent manner and maintained a high cell survival rate (>70%) in normal HUVECs; among them, SH-40 showed the lowest half-maximal inhibitory concentration (IC₅₀₎ value (1.09-3.89 mg/mL) (p < 0.05). SH-40 displayed the highest biological activity due to its higher molecular weight and characteristic monosaccharide ratio (p < 0.05), suggesting that molecular weight was one of the important factors influencing its function and activity. These findings provided a basis for the hierarchical preparation and differentiated application of polysaccharides isolated from Sanghuangporus vaninii.
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BibTeX
@article{guo2026gradient,
title = {{Gradient Ethanol Precipitation of Sanghuangporus vaninii Polysaccharides: Physicochemical Properties and Inhibition of Tumor Cell Proliferation.}},
author = {Xilei Guo and Jiayu Gu and Jiaxin Chu and Yuanqi Duan and Debin Wang and Hao Wu and Wei Zhou and Zhengyu Hu},
journal = {Foods (Basel, Switzerland)},
year = {2026},
month = sep,
doi = {10.3390/foods15193474},
url = {https://europepmc.org/article/MED/42883581},
note = {CC BY},
}
RIS
TY - JOUR
TI - Gradient Ethanol Precipitation of Sanghuangporus vaninii Polysaccharides: Physicochemical Properties and Inhibition of Tumor Cell Proliferation.
AU - Xilei Guo
AU - Jiayu Gu
AU - Jiaxin Chu
AU - Yuanqi Duan
AU - Debin Wang
AU - Hao Wu
AU - Wei Zhou
AU - Zhengyu Hu
PY - 2026
DA - 2026/09/28
JO - Foods (Basel, Switzerland)
DO - 10.3390/foods15193474
UR - https://europepmc.org/article/MED/42883581
AB - Three polysaccharide fractions (SH-40, SH-60, and SH-80) were sequentially separated from Sanghuangporus vaninii fruiting bodies through graded ethanol precipitation to investigate the influence of molecular weight on their structure, physicochemical properties, and inhibition of tumor cell proliferation. The results revealed that the molecular weights of these three fractions were approximately 5.90 × 10⁵, 4.82 × 10⁵, and 4.13 × 10⁵ Da, respectively; they shared similar functional group profiles, monosaccharide compositions and glycosidic bonds. As the molecular weight decreased, the water holding capacity (WHC) declined, while the water solubility index (WSI) and oil holding capacity (OHC) increased. In tumor cell proliferation inhibition assays, all three fractions inhibited the proliferation of MCF-7, HepG2, A549, and SGC-7901 cells in a concentration-dependent manner and maintained a high cell survival rate (>70%) in normal HUVECs; among them, SH-40 showed the lowest half-maximal inhibitory concentration (IC₅₀₎ value (1.09-3.89 mg/mL) (p < 0.05). SH-40 displayed the highest biological activity due to its higher molecular weight and characteristic monosaccharide ratio (p < 0.05), suggesting that molecular weight was one of the important factors influencing its function and activity. These findings provided a basis for the hierarchical preparation and differentiated application of polysaccharides isolated from Sanghuangporus vaninii.
SN - 2304-8158
ER -