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Peer-reviewedCC BYFrontiers in microbiology

Application and evaluation of nanopore targeted sequencing in the detection of bacterial and fungal pathogens in leukemia bloodstream infections.

  • Qian Yao1,2
  • Ling Tang3
  • Daozi Jiang3
  • Ming Wang1,2
  1. 1Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.
  2. 2Hubei Jiangxia Laboratory, Wuhan, China.
  3. 3Department of Hematology, Renmin Hospital of Wuhan University, Wuhan, China.
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Abstract

Objective

To evaluate the diagnostic performance and clinical impact of microbial nanopore-targeted sequencing (NTS) technology on pathogenic microorganisms in leukemia patients.Methods

In this retrospective observational study, we recruited 206 patients to study the effectiveness of NTS in the identification of infectious pathogens. The detection performance of NTS was compared with that of blood culture (BC).Results

The positive rate of NTS was significantly higher than that of BC [62.62% (129/206 cases) vs. 3.88% (8/206 cases)]. Compared with BC, the positive and negative percentage agreement of NTS were 87.50% (95%CI: 47.35-99.68%), (7 of 8 cases) and 38.38% (95%CI: 31.52-45.60%), (76 of 198 cases), respectively. The sensitivity of NTS for diagnosing bloodstream infections was 65.32% (95%CI: 56.21-73.69%), (81/124), which was significantly higher than BC [6.45% (95%CI: 2.84-12.13%), (8/124)], P < 0.001. The specificity of NTS was 41.46% (95%CI: 30.17-53.52%%), (34/82), and that of BC was [100%(95%CI: 95.56-100.00%) (82/82)]. The medication of 32 participants (24.81% [32/129]) was modified by NTS results. Among 206 participants, 29 cases who adjusted their medication based on NTS results showed improved clinical outcomes.Conclusion

NTS increases pathogen-detection yield but has low specificity and can be used as an adjunctive diagnostic method for leukemia BSI.

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Cite

BibTeXRIS
BibTeX
@article{yao2026application,
  title = {{Application and evaluation of nanopore targeted sequencing in the detection of bacterial and fungal pathogens in leukemia bloodstream infections.}},
  author = {Qian Yao and Ling Tang and Daozi Jiang and Ming Wang},
  journal = {Frontiers in microbiology},
  year = {2026},
  month = sep,
  doi = {10.3389/fmicb.2026.1866353},
  url = {https://europepmc.org/article/MED/42903871},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Application and evaluation of nanopore targeted sequencing in the detection of bacterial and fungal pathogens in leukemia bloodstream infections.
AU  - Qian Yao
AU  - Ling Tang
AU  - Daozi Jiang
AU  - Ming Wang
PY  - 2026
DA  - 2026/09/24
JO  - Frontiers in microbiology
DO  - 10.3389/fmicb.2026.1866353
UR  - https://europepmc.org/article/MED/42903871
AB  - Objective  To evaluate the diagnostic performance and clinical impact of microbial nanopore-targeted sequencing (NTS) technology on pathogenic microorganisms in leukemia patients.Methods  In this retrospective observational study, we recruited 206 patients to study the effectiveness of NTS in the identification of infectious pathogens. The detection performance of NTS was compared with that of blood culture (BC).Results  The positive rate of NTS was significantly higher than that of BC [62.62% (129/206 cases) vs. 3.88% (8/206 cases)]. Compared with BC, the positive and negative percentage agreement of NTS were 87.50% (95%CI: 47.35-99.68%), (7 of 8 cases) and 38.38% (95%CI: 31.52-45.60%), (76 of 198 cases), respectively. The sensitivity of NTS for diagnosing bloodstream infections was 65.32% (95%CI: 56.21-73.69%), (81/124), which was significantly higher than BC [6.45% (95%CI: 2.84-12.13%), (8/124)], P < 0.001. The specificity of NTS was 41.46% (95%CI: 30.17-53.52%%), (34/82), and that of BC was [100%(95%CI: 95.56-100.00%) (82/82)]. The medication of 32 participants (24.81% [32/129]) was modified by NTS results. Among 206 participants, 29 cases who adjusted their medication based on NTS results showed improved clinical outcomes.Conclusion  NTS increases pathogen-detection yield but has low specificity and can be used as an adjunctive diagnostic method for leukemia BSI.
SN  - 1664-302X
ER  -