Dynamic 2′-O-Me of rRNA regulates ribosome translation in Arabidopsis nucleolin1 mutants and during seed-to-seedling transition
Abstract
Across all kingdoms of life, 2′-O-ribose methylation (2′-O-Me) is the most abundant modification of ribosomal RNAs (rRNAs), which are central to ribosome assembly and function. The 2′-O-Me of specific rRNA sites is catalysed by C/D-box small nucleolar ribonucleoprotein (snoRNP) complexes. However, how and when the landscape of plant rRNA 2′-O-Me modifications is remodelled remains largely unexplored. To study the regulation of rRNA 2′-O-Me and its impact on ribosome translation, we characterised Arabidopsis NUCLEOLIN 1 (NUC1)-depleted plants, which exhibit reduced 2′-O-Me at dozens specific rRNA sites, and investigated the impact of 2′-O-Me in ribosome translation during plant development. We demonstrated that 2′-O-Me is dynamically regulated during seed-to-seedling transition. Our analysis identified differentially methylated rRNA sites, which are specifically located in structurally critical regions of the ribosome. Notably, this hypomethylation is uncoupled from the global expression, assembly, or subcellular localisation of C/D-box snoRNPs. We further observed that polysomes with low 2′-O-Me levels not only accumulate but also exhibit differential mRNA loading. These polysomes characterised by reduced 2'-O-Me at specific rRNA sites, influence multiples aspects of translation, including ribosomes stalling at the initiation, elongation and termination steps. Together, these findings advance our understanding of the dynamic regulation of rRNA 2′-O-methylation and its functional role in ribosome-mediated translation in plants.
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BibTeX
@misc{neumann2026dynamic,
title = {{Dynamic 2′-O-Me of rRNA regulates ribosome translation in Arabidopsis nucleolin1 mutants and during seed-to-seedling transition}},
author = {Sara Alina Neumann and Jacinthe Azevedo Favory and Marie-Christine Carpentier and Remy Merret and Virginie Marchand and Valerie Bourguignon and Pascale Comella and Anne de Bures and Magali Rompais and Laurence Drouard and Christine Carapito and Christine Gaspin and Yuri Motorin and Julio Sáez-Vásquez},
howpublished = {HAL},
year = {2026},
month = oct,
doi = {10.64898/2026.10.05.756201},
url = {https://hal.science/hal-05783377v1},
note = {CC BY-NC 4.0},
}
RIS
TY - UNPB
TI - Dynamic 2′-O-Me of rRNA regulates ribosome translation in Arabidopsis nucleolin1 mutants and during seed-to-seedling transition
AU - Sara Alina Neumann
AU - Jacinthe Azevedo Favory
AU - Marie-Christine Carpentier
AU - Remy Merret
AU - Virginie Marchand
AU - Valerie Bourguignon
AU - Pascale Comella
AU - Anne de Bures
AU - Magali Rompais
AU - Laurence Drouard
AU - Christine Carapito
AU - Christine Gaspin
AU - Yuri Motorin
AU - Julio Sáez-Vásquez
PY - 2026
DA - 2026/10/05
PB - HAL
DO - 10.64898/2026.10.05.756201
UR - https://hal.science/hal-05783377v1
AB - Across all kingdoms of life, 2′-O-ribose methylation (2′-O-Me) is the most abundant modification of ribosomal RNAs (rRNAs), which are central to ribosome assembly and function. The 2′-O-Me of specific rRNA sites is catalysed by C/D-box small nucleolar ribonucleoprotein (snoRNP) complexes. However, how and when the landscape of plant rRNA 2′-O-Me modifications is remodelled remains largely unexplored. To study the regulation of rRNA 2′-O-Me and its impact on ribosome translation, we characterised Arabidopsis NUCLEOLIN 1 (NUC1)-depleted plants, which exhibit reduced 2′-O-Me at dozens specific rRNA sites, and investigated the impact of 2′-O-Me in ribosome translation during plant development. We demonstrated that 2′-O-Me is dynamically regulated during seed-to-seedling transition. Our analysis identified differentially methylated rRNA sites, which are specifically located in structurally critical regions of the ribosome. Notably, this hypomethylation is uncoupled from the global expression, assembly, or subcellular localisation of C/D-box snoRNPs. We further observed that polysomes with low 2′-O-Me levels not only accumulate but also exhibit differential mRNA loading. These polysomes characterised by reduced 2'-O-Me at specific rRNA sites, influence multiples aspects of translation, including ribosomes stalling at the initiation, elongation and termination steps. Together, these findings advance our understanding of the dynamic regulation of rRNA 2′-O-methylation and its functional role in ribosome-mediated translation in plants.
ER -