Investigation of Extracellular Vesicle Corona Formation with Label-Free Optical Waveguide Lightmode Spectroscopy Method.
10 affiliations
- 1Pediatric Center, MTA Center of Excellence, Semmelweis University, 1083 Budapest, Hungary.
- 2HUN-REN-SU Pediatrics and Nephrology Research Group, 1083 Budapest, Hungary.
- 3Institute of Genetics, Cell- and Immunobiology, Semmelweis University, 1089 Budapest, Hungary.
- 4Department of Plant Physiology and Molecular Plant Biology, ELTE Eötvös Loránd University, 1053 Budapest, Hungary.
- 5Biological Nanochemistry Research Group, Institute of Materials and Environmental Chemistry, HUN-REN Research Centre for Natural Sciences, 1117 Budapest, Hungary.
- 6Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, 1111 Budapest, Hungary.
- 7HUN-REN Office for Supported Research Groups, Department of Biophysics and Radiation Biology, Faculty of Medicine, Semmelweis University, 1094 Budapest, Hungary.
- 8Cantabio Pharmaceuticals Kft., 1149 Budapest, Hungary.
- 9HUN-REN SU, Translational Extracellular Vesicles Research Group, 1089 Budapest, Hungary.
- 10Insulinde BT., 1111 Budapest, Hungary.
Abstract
Extracellular vesicles (EVs) are lipid bilayer-delimited particles that are naturally released from all types of cells into the extracellular environment. During their release to the extracellular space, EVs adsorb biomolecules, collectively designated as EV corona. In this study, we developed a novel method for immobilizing EVs and assessing their capacity to bind biomolecules, using human serum albumin (HSA) as a model molecule for corona acquisition and optical waveguide lightmode spectroscopy as the analytical technique. Ten ng/mL poly-L-lysine (15-30 kDa), prepared in 42 mM TRIS buffer at pH 7.4, was used to achieve non-specific immobilization of EVs at a concentration of 4.5 × 10¹⁰ particles/mL, and also to provide surface passivation against proteins. The experiments were performed at 25 °C with a 100 µL/min flow rate. The optimal HSA detection range was between 1 and 100 ng/mL with EC₅₀ = 2.39 ng/mL. A mathematical model was also established to determine the estimated number of HSA molecules that can be bound on the surface of EVs. In saturation experiments it was demonstrated that the magnitude of HSA bound on the surface of EVs corresponded to the theoretical estimate. After its development, the method was further tested using human plasma and a chosen protein (DJ-1) as well as non-protein corona components, specifically double-stranded DNA and a microRNA (miR494).
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BibTeX
@article{bokrossy2026investigation,
title = {{Investigation of Extracellular Vesicle Corona Formation with Label-Free Optical Waveguide Lightmode Spectroscopy Method.}},
author = {Péter Bokrossy and Beáta Szebeni and Tamás Visnovitz and Dorina Lenzinger and Éva Pállinger and Nóra Fekete and András I Försönits and Zoltán Varga and Judith Mihály and Balázs Fórizs and Domonkos Pap and Apor Veres-Székely and Csenge Szász and Jamila Raufi and Edit I Buzás and Attila J Szabó and Ádám Vannay and Nóra Adányi},
journal = {Biosensors},
year = {2026},
month = oct,
doi = {10.3390/bios16100550},
url = {https://europepmc.org/article/MED/42930097},
note = {CC BY},
}
RIS
TY - JOUR
TI - Investigation of Extracellular Vesicle Corona Formation with Label-Free Optical Waveguide Lightmode Spectroscopy Method.
AU - Péter Bokrossy
AU - Beáta Szebeni
AU - Tamás Visnovitz
AU - Dorina Lenzinger
AU - Éva Pállinger
AU - Nóra Fekete
AU - András I Försönits
AU - Zoltán Varga
AU - Judith Mihály
AU - Balázs Fórizs
AU - Domonkos Pap
AU - Apor Veres-Székely
AU - Csenge Szász
AU - Jamila Raufi
AU - Edit I Buzás
AU - Attila J Szabó
AU - Ádám Vannay
AU - Nóra Adányi
PY - 2026
DA - 2026/10/01
JO - Biosensors
DO - 10.3390/bios16100550
UR - https://europepmc.org/article/MED/42930097
AB - Extracellular vesicles (EVs) are lipid bilayer-delimited particles that are naturally released from all types of cells into the extracellular environment. During their release to the extracellular space, EVs adsorb biomolecules, collectively designated as EV corona. In this study, we developed a novel method for immobilizing EVs and assessing their capacity to bind biomolecules, using human serum albumin (HSA) as a model molecule for corona acquisition and optical waveguide lightmode spectroscopy as the analytical technique. Ten ng/mL poly-L-lysine (15-30 kDa), prepared in 42 mM TRIS buffer at pH 7.4, was used to achieve non-specific immobilization of EVs at a concentration of 4.5 × 10¹⁰ particles/mL, and also to provide surface passivation against proteins. The experiments were performed at 25 °C with a 100 µL/min flow rate. The optimal HSA detection range was between 1 and 100 ng/mL with EC₅₀ = 2.39 ng/mL. A mathematical model was also established to determine the estimated number of HSA molecules that can be bound on the surface of EVs. In saturation experiments it was demonstrated that the magnitude of HSA bound on the surface of EVs corresponded to the theoretical estimate. After its development, the method was further tested using human plasma and a chosen protein (DJ-1) as well as non-protein corona components, specifically double-stranded DNA and a microRNA (miR494).
SN - 2079-6374
ER -