Label-free volumetric dynamic atlas of fresh mouse organs using dynamic full-field optical coherence tomography
- 1State Key Laboratory of Lowdimensional Quantum Physics and Department of Physics, Tsinghua University, 100084, Beijing, China
Abstract
Dynamic full-field optical coherence tomography (D-FFOCT) enables label-free visualization of tissue microarchitecture and endogenous intracellular dynamics. Here, we establish a volumetric atlas of ten fresh mouse organs using a mobile D-FFOCT imaging platform. The system enables large-area imaging of approximately 1 cm2 within 15 min with 1,024 frames per field of view, achieving 0.75-{micro}m lateral resolution and imaging depths of up to 200 {micro}m. Large-area mosaics, three-dimensional reconstructions, and comparisons with conventional histology reveal diverse organ-specific tissue architectures. Beyond morphological characterization, quantitative analysis of dynamic intensity and mean fluctuation frequency across nine organs reveals distinct dynamic signatures and tissue heterogeneity. Longitudinal imaging of brain, liver, and intestinal tissues further demonstrates organ-dependent temporal changes in endogenous dynamics following excision. Finally, we publicly release raw D-FFOCT imaging datasets from all ten organs, providing an open resource for quantitative tissue characterization, algorithm development, and reproducible research. Together, this study provides a comprehensive, openly accessible optical atlas of fresh mouse organs, establishing a foundation for systematic tissue characterization and advancing the broader application of D-FFOCT in biomedical research.
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BibTeX
@misc{zhang2026labelfree,
title = {{Label-free volumetric dynamic atlas of fresh mouse organs using dynamic full-field optical coherence tomography}},
author = {Q. Zhang and M. Liu and H. Cui and S. Lin and C. Wang and J. Xie and L. Zhang and H. Ma and C. Ma and P. Xue and B. He},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.28.762177},
url = {https://www.biorxiv.org/content/10.64898/2026.10.28.762177v1},
note = {CC BY-NC},
}
RIS
TY - UNPB
TI - Label-free volumetric dynamic atlas of fresh mouse organs using dynamic full-field optical coherence tomography
AU - Q. Zhang
AU - M. Liu
AU - H. Cui
AU - S. Lin
AU - C. Wang
AU - J. Xie
AU - L. Zhang
AU - H. Ma
AU - C. Ma
AU - P. Xue
AU - B. He
PY - 2026
DA - 2026/10/28
PB - bioRxiv
DO - 10.64898/2026.10.28.762177
UR - https://www.biorxiv.org/content/10.64898/2026.10.28.762177v1
AB - Dynamic full-field optical coherence tomography (D-FFOCT) enables label-free visualization of tissue microarchitecture and endogenous intracellular dynamics. Here, we establish a volumetric atlas of ten fresh mouse organs using a mobile D-FFOCT imaging platform. The system enables large-area imaging of approximately 1 cm2 within 15 min with 1,024 frames per field of view, achieving 0.75-{micro}m lateral resolution and imaging depths of up to 200 {micro}m. Large-area mosaics, three-dimensional reconstructions, and comparisons with conventional histology reveal diverse organ-specific tissue architectures. Beyond morphological characterization, quantitative analysis of dynamic intensity and mean fluctuation frequency across nine organs reveals distinct dynamic signatures and tissue heterogeneity. Longitudinal imaging of brain, liver, and intestinal tissues further demonstrates organ-dependent temporal changes in endogenous dynamics following excision. Finally, we publicly release raw D-FFOCT imaging datasets from all ten organs, providing an open resource for quantitative tissue characterization, algorithm development, and reproducible research. Together, this study provides a comprehensive, openly accessible optical atlas of fresh mouse organs, establishing a foundation for systematic tissue characterization and advancing the broader application of D-FFOCT in biomedical research.
ER -