Silane-Based Methyl- and Carboxyl-Functionalized Mica Substrates for High-Speed Atomic Force Microscopy.
9 affiliations
- 1Division of Mathematical and Physical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.
- 2Division of Material Chemistry, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.
- 3Division of Nano Life Science, Graduate School of Frontier Science Initiative, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.
- 4WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.
- 5Faculty of Frontier Engineering, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa920-1192, Japan.
- 6Department of Bacteriology, Niigata University School of Medicine, 1-757 Asahimachi-dori, Chuo-ku, Niigata951-8510, Japan.
- 7Department of Bacteriology, Osaka Metropolitan University Graduate School of Medicine, 1-4-3 Asahi-Machi, Abeno-ku, Osaka545-8586, Japan.
- 8Division of Research Aids, Hokkaido University Institute for Vaccine Research & Development, Kita 20, Nishi 10, Kita-Ku, Sapporo, Hokkaido001-0020, Japan.
- 9Laboratory of Tuberculosis, Institute of Tropical Disease, Universitas Airlangga, Kampus C JI. Mulyorejo, Surabaya60113, Indonesia.
Abstract
High-speed atomic force microscopy (HS-AFM) enables visualization of biomolecular dynamics in solution with high spatiotemporal resolution but requires substrates that support stable adsorption without perturbing their structure or dynamics. Mica is widely used as an HS-AFM substrate because it provides an atomically flat surface. Several methods have been proposed to functionalize mica surfaces, but they are not suitable for all biomolecules. Here, we expand the HS-AFM substrate toolbox by introducing methyl- and carboxyl (COOH)-functionalized mica surfaces based on a common silanization strategy. In particular, a COOH-functionalized mica substrate was prepared via a mild thiol-maleimide coupling reaction, enabling efficient surface modification under aqueous conditions. Using proteins with different charges and shapes, we evaluated adsorption and diffusion behaviors on these substrates by HS-AFM. Distinct differences in molecular mobility and binding were observed depending on surface properties. These results provide practical guidelines for substrate selection and broaden the applicability of HS-AFM to diverse biomolecular systems.
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BibTeX
@article{nakamoto2026silanebased,
title = {{Silane-Based Methyl- and Carboxyl-Functionalized Mica Substrates for High-Speed Atomic Force Microscopy.}},
author = {Kaho Nakamoto and Yuna Goto and Sohta Yamamoto and Hikaru Ichida and Kenichi Umeda and Keisuke Miyazawa and Takeshi Fukuma and Akihito Nishiyama and Sohkichi Matsumoto and Hajime Maeda and Noriyuki Kodera},
journal = {Langmuir : the ACS journal of surfaces and colloids},
year = {2026},
month = sep,
doi = {10.1021/acs.langmuir.6c02731},
url = {https://europepmc.org/article/MED/42917971},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Silane-Based Methyl- and Carboxyl-Functionalized Mica Substrates for High-Speed Atomic Force Microscopy.
AU - Kaho Nakamoto
AU - Yuna Goto
AU - Sohta Yamamoto
AU - Hikaru Ichida
AU - Kenichi Umeda
AU - Keisuke Miyazawa
AU - Takeshi Fukuma
AU - Akihito Nishiyama
AU - Sohkichi Matsumoto
AU - Hajime Maeda
AU - Noriyuki Kodera
PY - 2026
DA - 2026/09/01
JO - Langmuir : the ACS journal of surfaces and colloids
DO - 10.1021/acs.langmuir.6c02731
UR - https://europepmc.org/article/MED/42917971
AB - High-speed atomic force microscopy (HS-AFM) enables visualization of biomolecular dynamics in solution with high spatiotemporal resolution but requires substrates that support stable adsorption without perturbing their structure or dynamics. Mica is widely used as an HS-AFM substrate because it provides an atomically flat surface. Several methods have been proposed to functionalize mica surfaces, but they are not suitable for all biomolecules. Here, we expand the HS-AFM substrate toolbox by introducing methyl- and carboxyl (COOH)-functionalized mica surfaces based on a common silanization strategy. In particular, a COOH-functionalized mica substrate was prepared via a mild thiol-maleimide coupling reaction, enabling efficient surface modification under aqueous conditions. Using proteins with different charges and shapes, we evaluated adsorption and diffusion behaviors on these substrates by HS-AFM. Distinct differences in molecular mobility and binding were observed depending on surface properties. These results provide practical guidelines for substrate selection and broaden the applicability of HS-AFM to diverse biomolecular systems.
SN - 0743-7463
SN - 1520-5827
ER -