GSDMD transcript levels are higher in endothelial than cardiomyocyte compartments across three human heart cohorts
- 1Department of Internal Medicine, Kyushu University Beppu Hospital, Beppu, Japan.
Abstract
Cardiac GSDMD research has largely centred on cardiomyocytes. We quantified donor-level endothelial-minus-cardiomyocyte differences in two single-nucleus cohorts and one GeoMx spatial cohort, retaining assay-specific scales. GSDMD was higher in endothelial compartments in 42/42, 38/38 and 27/32 donors and ranked, post hoc, at the 94.9th, 95.1st and 97.4th percentiles of the corresponding within-cohort signed-contrast distributions. Although positive direction and complete donor concordance were not unique transcriptome-wide, this hypothesis-led target retained high rank across markedly different reference backgrounds and assay modalities. A fixed eight-transcript panel included both endothelial- and cardiomyocyte-shifted members, arguing against a uniform panel-wide directional shift or simple detection failure. GeoMx provided the most discriminating transcriptome background but remained composition-sensitive. GSDMD transcripts are therefore recurrently higher in endothelial than cardiomyocyte compartments across human-heart cohorts, showing that a cardiomyocyte-only interpretation of human-heart GSDMD transcript signals is incomplete.
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BibTeX
@misc{oryoji2026gsdmd,
title = {{GSDMD transcript levels are higher in endothelial than cardiomyocyte compartments across three human heart cohorts}},
author = {D. Oryoji and G. Doi and S. Fujimoto and N. Nishimura and K. Otsuka and A. Kuwahara and M. Ayano and Y. Kimoto and K. Akashi and H. Niiro and H. Mitoma},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.06.749635},
url = {https://www.biorxiv.org/content/10.64898/2026.09.06.749635v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - GSDMD transcript levels are higher in endothelial than cardiomyocyte compartments across three human heart cohorts
AU - D. Oryoji
AU - G. Doi
AU - S. Fujimoto
AU - N. Nishimura
AU - K. Otsuka
AU - A. Kuwahara
AU - M. Ayano
AU - Y. Kimoto
AU - K. Akashi
AU - H. Niiro
AU - H. Mitoma
PY - 2026
DA - 2026/09/06
PB - bioRxiv
DO - 10.64898/2026.09.06.749635
UR - https://www.biorxiv.org/content/10.64898/2026.09.06.749635v1
AB - Cardiac GSDMD research has largely centred on cardiomyocytes. We quantified donor-level endothelial-minus-cardiomyocyte differences in two single-nucleus cohorts and one GeoMx spatial cohort, retaining assay-specific scales. GSDMD was higher in endothelial compartments in 42/42, 38/38 and 27/32 donors and ranked, post hoc, at the 94.9th, 95.1st and 97.4th percentiles of the corresponding within-cohort signed-contrast distributions. Although positive direction and complete donor concordance were not unique transcriptome-wide, this hypothesis-led target retained high rank across markedly different reference backgrounds and assay modalities. A fixed eight-transcript panel included both endothelial- and cardiomyocyte-shifted members, arguing against a uniform panel-wide directional shift or simple detection failure. GeoMx provided the most discriminating transcriptome background but remained composition-sensitive. GSDMD transcripts are therefore recurrently higher in endothelial than cardiomyocyte compartments across human-heart cohorts, showing that a cardiomyocyte-only interpretation of human-heart GSDMD transcript signals is incomplete.
ER -